Streptococcus equi subsp. zooepidemicus infections associated with guinea pigs.

Streptococcus equi subsp. zooepidemicus is a known zoonotic pathogen. In this public health investigation conducted in Virginia, USA, in 2013, we identified a probable family cluster of S. zooepidemicus cases linked epidemiologically and genetically to infected guinea pigs. S. zooepidemicus infections should be considered in patients who have severe clinical illness and report guinea pig exposure.

Streptococcus equi subsp. zooepidemicus is a facultative pathogen affecting animals and humans. Infections have occurred in horses, pigs, ruminants, guinea pigs, monkeys, cats, and dogs (,). Zoonotic transmission of S. zooepidemicus is rare and is usually associated with drinking unpasteurized milk or through contact with horses by persons who usually have underlying health conditions (–). Few if any human case-patients with S. zooepidemicus infection have documented guinea pig exposure even though S. zooepidemicus infections have been described in guinea pigs since 1907 (). This case report describes 1 probable and 1 confirmed human case of severe S. zooepidemicus infection and the laboratory methods used to link human and guinea pig isolates.

Case Reports

An adult man (patient 1) from northern Virginia, USA arrived at a hospital in late February 2013 with influenza-like symptoms, worsening bilateral thigh pain and stiffness, nausea, shivering, fatigue, diarrhea, sweating, and headache. Past medical history included exercise-induced asthma, nephrolithiasis, and slightly elevated liver function test results for “a couple of years,” as stated in the medical record. Initial physical examination of the patient revealed mild scleral icterus, rhabdomyolysis, and rash on his thighs. The patient’s elevated liver function test results were attributed to the rhabdomyolysis. Shortly after being admitted, the patient experienced acute renal failure, sepsis, pneumonia, and bilateral lower extremity edema, the latter of which was thought to be compartment syndrome. Blood cultures performed at the hospital showed group C streptococcal infection. Because of the patient’s worsening condition, he was transferred to a tertiary care center. At the tertiary care center, the patient was treated for septic shock secondary to rhabdomyolysis, placed on a ventilator several times to treat respiratory failure, and underwent bilateral thigh fasciotomy and debridement several times to treat necrotizing fasciitis. Wound cultures identified S. equi as the causative agent; a subspecies was not specified, although zooepidemicus was likely because it is the only zoonotic subspecies of S. equi. After treatment at the tertiary facility for several months, the patient was discharged to a rehabilitation hospital for another month.

An elderly man from central Virginia (patient 2) who was related to patient 1 was admitted to a hospital, 1 week after patient 1 was hospitalized, with nausea, vomiting, chills, difficulty breathing, weakness, abdominal and chest pain, and icterus. The medical history of patient 2 included smoking, oral cancer, myocardial infarction, hypertension, hyperlipidemia, and coronary artery disease. By the second day in the hospital, patient 2 experienced acute hypoxia and respiratory failure; pneumonia in the right lower lobe was diagnosed. He also had hypotension secondary to septic shock and multiple organ failure. Group C Streptococcus spp. were identified in blood cultures 2 days after hospitalization. Patient 2 was discharged 18 days after hospitalization and was receiving continuous oxygen.

The local health department for the area in which patient 1 resided was contacted by the tertiary care center where he was treated because of the probability that the S. equi infection was caused by guinea pig exposure. Questioning of a female relative of patient 1 (relative 1) indicated that patient 1 recently purchased 4 guinea pigs and that 1 had died shortly after purchase. During the interview with relative 1, it was learned that patient 2 was hospitalized. After another female relative (relative 2) was interviewed, it was learned that patient 2 had cleaned the guinea pigs’ enclosure 2 days before his illness. The Virginia Department of Health (VDH) requested isolates from both patients to be forwarded to the Division of Consolidated Laboratory Services (DCLS), the state public health laboratory. Only an isolate from patient 2 was available and forwarded. VDH representatives also discussed the likelihood of disease in patients 1 and 2 being caused by the guinea pigs with the relatives. Relative 2, who was caring for the guinea pigs at the time, relinquished the 3 remaining guinea pigs which were sent to a Virginia Department of Agriculture and Consumer Services laboratory for euthanasia and microbiological testing.

Using API 20 Strep strips (bioMérieux, Inc., Durham, NC, USA), laboratory personnel identified S. zooepidemicus in isolates from 5 specimens (1 lymph node, 2 conjunctival swabs, and 2 nasal passage swabs) collected from 2 of the 3 guinea pigs. No apparent lesions were noted in the guinea pigs upon euthanasia and sample collection. Three isolates from the guinea pigs, 1 from each site, were forwarded to DCLS.

At DCLS, patient 2’s isolate was also identified as S. zooepidemicus by API 20 Strep strips. DCLS staff performed pulsed-field gel electrophoresis (PFGE) testing on all 4 isolates. Briefly, plugs were prepared by incubating washed cells in Tris-EDTA buffer solution (pH 8.0) with 10 μL lysozyme (10 mg/mL) before mixing with 1.5% molecular grade agarose and 0.17 mg/mL proteinase K (Roche, Mannheim, Germany) (). Cells were lysed in extracellular buffer with 1 mg/mL lysozyme and 0.3 mg/mL proteinase K, for 1.5 h at 37°C (). After a MilliQ water wash and 3 Tris-EDTA buffer washes, DNA was digested with 100 U SmaI and 100 U ApaI both from (New England Biolabs, Inc., Ipswitch, MA, USA) and separated on a 1% agarose gel at 6 V/min, 14°C for 17.5–18 h in 0.5× TBE buffer by using a CHEF Mapper XRS electrophoresis system (Bio-Rad, Hercules, CA, USA). The running parameters were as follows: for SmaI: initial pulse, 5.3 sec; final pulse, 34.9 sec; ApaI: initial pulse, 2.0 sec; final pulse, 25 sec. The gels were visualized by using ImageLab (BioRad) software with a ChemiDoc XR+ (BioRad). An Enterococcus faecalis control strain was used for image normalization during DNA fingerprint pattern analysis with BIONUMERICS 5.1 software (Applied Maths, Austin, TX, USA). The banding patterns were compared by using Dice coefficients with a 1.0%–1.5% band tolerance. PFGE results indicated that all 4 isolates were indistinguishable by comparison with the SmaI and ApaI enzymes (Figure).

Figure

Pulsed-field gel electrophoresis (PFGE) patterns for 4 Streptococcus equi subsp. zooepidemicus isolates from 1 person and 3 guinea pigs submitted to the Division of Consolidated Laboratory Services, Virginia, USA. Patterns indicate that all 4 isolates were indistinguishable by the SmaI and ApaI enzymes. Specimen origin and type are indicated.

Multilocus sequence typing was performed as described (http://pubmlst.org/szooepidemicus/) based on the allelic profile of 7 housekeeping gene fragments. The isolates from patient 2 and the 2 guinea pigs shared the same 6-locus profile of 370- to 459-bp housekeeping gene sequences. The 6-locus profile, arcC27, nrdE19, spi45, tdK1, tpi34, yqiL46, was found in each of the 4 isolates rather than a normally obtained 7-locus profile because the proS locus did not amplify. This 6-locus profile was unique: its closest match in the S. zooepidemicus database was ST194 (arcC27, nrdE3, proS1, spi45, tdk1, tpi34, yqiL46); both had 5 of the 6 gene-fragment sequences (Table). ST194 is recorded in the S. zooepidemicus database from 2 human blood isolates recovered during 2001. We subsequently found that ST194 is also shared by ATCC S. zooepidemicus strain 35246, which was isolated from a diseased pig in China ().

Table

Closest species matches to multilocus sequence typing alleles of study Streptococcus equi subsp. zooepidemicus isolates

Allele*	Length, bp	Closest GenBank overall species match (% identity/overlap)†	Next best GenBank matches (% identity/overlap)†
arcC27	437	S. equi subsp. zooepidemicus strains CY, ATCC35246 (100%/437 bp); H70 (99%/437 bp); MHCS10565 (97%/437 bp)	S. equi subsp. equi strain 4047 (97%/437 bp)
nrdE19	448	S. equi subsp. zooepidemicus strains CY, ATCC35246, MGCS10565 (99%/448 bp); H70 (97%/448 bp)	S. equi subsp. equi strain 4047 (94%/448 bp); S. thermophilus ASCC1275,JIM8232,ND03, 5 others (80%/447 bp)
spi45	459	S. equi subsp. zooepidemicus strains CY, ATCC35246,4047 (100%/459 bp); MGCS10565 (99%/459 bp); H70 (94%/459 bp)	S. dysgalactiae subsp. equisimilis strains ATCC12394, 167, AC2713, RE378, GGS124 (72%/447 bp)
tdk1	370	S. equi subsp. zooepidemicus strains CY, 636199–1A, 65843, ATCC35246, H70 (100%/370 bp); 15 others (99%/370 bp)	S. equi subsp. equi strain 4047 (99%/370bp); S. equi subsp. zooepidemicus 10 strains (96%–98%/369 bp)
tpi34	424	S. equi subsp. zooepidemicus strains CY, 14580–1A, 44464, ATCC35246 (100%/424bp); 22 others (99%/424 bp); 27 others (95%–97%/424 bp)	S. equi subsp. equi strain 4047 (97%/424 bp); S. pyogenes 20 strains (82%/422 bp)
yqiL46	396	S. equi subsp. zooepidemicus strains CY, ATCC35246 (100%/396 bp); >30 strains (99%/396 bp); >20 strains (95%–98%/396 bp)	S. equi subsp. equi strain 4047 (97%/424 bp); S. pyogenes MGAS6180 (70%/422 bp)

*All 4 isolates shared the identical arcC27, nrdE19, spi45, tdk1, tpi34, yqiL46 alleles; sequences provided at http://pubmlst.org/szooepidemicus.
†Searches performed at http://blast.ncbi.nlm.nih.gov/Blast.cgi

In addition, the Centers for Disease Control and Prevention laboratory staff sequenced the so-called M-like protein gene (szp) of these isolates, using previously described sequencing and amplification primers (). The 1,128-bp structural gene sequence (GenBank accession no. KF722996) was found to be identical for the 4 isolates and closely matched the 1,140-bp M-like gene (szp) from the ATCC 35246 strain. The only difference was deletion of 1 of 10 consecutive 4-codon repeats (PKPE, codons 277–280) ().

Conclusions

S. zooepidemicus infection should be considered in patients who have purulent wounds or systemic symptoms of infection who have had known contact with guinea pigs or their environment. Likewise, patients whose specimen cultures reveal S. equi or further test results show S. zooepidemicus should be questioned about guinea pig exposure as well as exposure to other animals associated with this pathogen: horses, pigs, ruminants, monkeys, cats, and dogs.