Parim Brahma Naidu1, Ponnusamy Ponmurugan2, Mustapha Sabana Begum3, Karthick Mohan4, Balaji Meriga1, Ramavat RavindarNaik5, Ganapathy Saravanan6. 1. Department of Biochemistry, Animal physiology & Biochemistry Laboratory, Sri Venkateswara University, Tirupati, 517502, India. 2. Department of Biotechnology, K.S. Rangasamy College of Technology, Thokkavadi, Tiruchengode, Tamil Nadu, India. 3. Department of Biochemistry, Muthayammal College of Arts and Science, Rasipuram, Tamil Nadu, India. 4. Department of Biochemistry, St. Josephs College, Tiruchirappalli, Tamil Nadu, India. 5. National Center for Laboratory Animal Sciences, National Institute of Nutrition (ICMR) New Delhi, India. 6. Department of Biochemistry, Centre for Biological Science, K.S. Rangasamy College of Arts and Science, Thokkavadi, Tiruchengode, Tamil Nadu, India.
Abstract
BACKGROUND: Diabetes is often connected with significant morbidity, mortality and also has a pivotal role in the development of cardiovascular diseases. Diet intervention, particularly naturaceutical antioxidants have anti-diabetic potential and avert oxidative damage linked with diabetic pathogenesis. The present study investigated the effects of diosgenin, a saponin from fenugreek, on the changes in lipid profile in plasma, liver, heart and brain in high-fat diet-streptozotocin (HFD-STZ)-induced diabetic rats. Diosgenin was administered to HFD-STZ induced diabetic rats by orally at 60 mg kg(-1) body weight for 30 days to assess its effects on body weight gain, glucose, insulin, insulin resistance and cholesterol, triglycerides, free fatty acids and phospholipids in plasma, liver, heart and brain. RESULTS: The levels of body weight, glucose, insulin, insulin resistance, cholesterol, triglycerides, free fatty acids, phospholipids, VLDL-C and LDL-C were increased significantly (P < 0.05) whereas HDL-C level decreased in the HFD/STZ diabetic rats. Administration of diosgenin to HFD-STZ diabetic rats caused a decrease in body weight gain, blood glucose, insulin, insulin resistance and also it modulated lipid profile in plasma and tissues. CONCLUSION: The traditional plant fenugreek and its constituents mediate its anti-diabetic potential through mitigating hyperglycaemic status, altering insulin resistance by alleviating metabolic dysregulation of lipid profile in both plasma and tissues.
BACKGROUND:Diabetes is often connected with significant morbidity, mortality and also has a pivotal role in the development of cardiovascular diseases. Diet intervention, particularly naturaceutical antioxidants have anti-diabetic potential and avert oxidative damage linked with diabetic pathogenesis. The present study investigated the effects of diosgenin, a saponin from fenugreek, on the changes in lipid profile in plasma, liver, heart and brain in high-fat diet-streptozotocin (HFD-STZ)-induced diabeticrats. Diosgenin was administered to HFD-STZ induced diabeticrats by orally at 60 mg kg(-1) body weight for 30 days to assess its effects on body weight gain, glucose, insulin, insulin resistance and cholesterol, triglycerides, free fatty acids and phospholipids in plasma, liver, heart and brain. RESULTS: The levels of body weight, glucose, insulin, insulin resistance, cholesterol, triglycerides, free fatty acids, phospholipids, VLDL-C and LDL-C were increased significantly (P < 0.05) whereas HDL-C level decreased in the HFD/STZdiabeticrats. Administration of diosgenin to HFD-STZdiabeticrats caused a decrease in body weight gain, blood glucose, insulin, insulin resistance and also it modulated lipid profile in plasma and tissues. CONCLUSION: The traditional plant fenugreek and its constituents mediate its anti-diabetic potential through mitigating hyperglycaemic status, altering insulin resistance by alleviating metabolic dysregulation of lipid profile in both plasma and tissues.
Authors: P Rameshreddy; V V Sathibabu Uddandrao; Parim Brahmanaidu; S Vadivukkarasi; Ramavat Ravindarnaik; Pothani Suresh; K Swapna; A Kalaivani; Parimi Parvathi; P Tamilmani; Ganapathy Saravanan Journal: Mol Cell Biochem Date: 2017-10-09 Impact factor: 3.396