Hsu-Chung Liu1, Min-Chi Lu2, Yi-Chun Lin3, Tzu-Chin Wu4, Jeng-Yuan Hsu5, Ming-Shiou Jan6, Chuan-Mu Chen7. 1. Division of Chest Medicine, Department of Internal Medicine, Chung Shan Medical University Hospital and Cheng Ching Hospital, Taichung, Taiwan; School of Medicine, Chung Shan Medical University, Taichung, Taiwan; Department of Life Sciences, Agricultural Biotechnology Center, National Chung Hsing University, Taichung, Taiwan. 2. Institute of Microbiology and Immunology, Chung Shan Medical University, Taichung, Taiwan. 3. Department of Life Sciences, Agricultural Biotechnology Center, National Chung Hsing University, Taichung, Taiwan. 4. Division of Chest Medicine, Department of Internal Medicine, Chung Shan Medical University Hospital and Cheng Ching Hospital, Taichung, Taiwan; School of Medicine, Chung Shan Medical University, Taichung, Taiwan. 5. Division of Chest Medicine, Department of Internal Medicine, Taichung Veterans General Hospital, Taichung, Taiwan; Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan. 6. Institute of Microbiology and Immunology, Chung Shan Medical University, Taichung, Taiwan. Electronic address: mingshiou.jan@gmail.com. 7. Department of Life Sciences, Agricultural Biotechnology Center, National Chung Hsing University, Taichung, Taiwan. Electronic address: chchen1@dragon.nchu.edu.tw.
Abstract
BACKGROUND/ PURPOSE: The collection of exhaled breath condensate (EBC) is a noninvasive method that can be used to monitor the inflammatory status of patients with chronic airway diseases. We aimed to study differences in cytokine expression between patients with exacerbations of chronic obstructive pulmonary disease (COPD) and patients with asthma attacks. METHODS: Using a custom-made device and methods based on American Thoracic Society (ATS)/European Respiratory Society (ERS) recommendations, EBC samples were collected from nine COPD patients, 12 asthma patients and 10 healthy individuals. Cytokine concentrations in serum and EBC were measured via commercial ELISA kits. RESULTS: Of four cytokines measured in EBC [interleukin-8 (IL-8), IL-17, IL-4 and tumor necrosis factor-α (TNF-α)], only IL-8 was significantly higher in COPD than in asthma patients (5.27 ± 0.18 vs. 4.36 ± 0.34 pg/mL, p = 0.001). Moreover, COPD patients had higher serum IL-8 than asthma patients (10.57 ± 0.55 vs. 5.15 ± 0.24 pg/mL, p < 0.001). No significant correlation between serum and EBC cytokine concentrations was observed in each subgroup of patients. CONCLUSION: Compared with patients with asthma attacks, patients with exacerbated COPD had increased IL-8 expression in both serum and EBC. These results suggest that IL-8 may be more important in airway and systemic inflammation in COPD exacerbations than in asthma attacks.
BACKGROUND/ PURPOSE: The collection of exhaled breath condensate (EBC) is a noninvasive method that can be used to monitor the inflammatory status of patients with chronic airway diseases. We aimed to study differences in cytokine expression between patients with exacerbations of chronic obstructive pulmonary disease (COPD) and patients with asthma attacks. METHODS: Using a custom-made device and methods based on American Thoracic Society (ATS)/European Respiratory Society (ERS) recommendations, EBC samples were collected from nine COPDpatients, 12 asthmapatients and 10 healthy individuals. Cytokine concentrations in serum and EBC were measured via commercial ELISA kits. RESULTS: Of four cytokines measured in EBC [interleukin-8 (IL-8), IL-17, IL-4 and tumor necrosis factor-α (TNF-α)], only IL-8 was significantly higher in COPD than in asthmapatients (5.27 ± 0.18 vs. 4.36 ± 0.34 pg/mL, p = 0.001). Moreover, COPDpatients had higher serum IL-8 than asthmapatients (10.57 ± 0.55 vs. 5.15 ± 0.24 pg/mL, p < 0.001). No significant correlation between serum and EBC cytokine concentrations was observed in each subgroup of patients. CONCLUSION: Compared with patients with asthma attacks, patients with exacerbated COPD had increased IL-8 expression in both serum and EBC. These results suggest that IL-8 may be more important in airway and systemic inflammation in COPD exacerbations than in asthma attacks.
Authors: Bouchra Assarag; Bruno Dujardin; Alexandre Delamou; Fatima-Zahra Meski; Vincent De Brouwere Journal: PLoS One Date: 2015-01-22 Impact factor: 3.240
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