Kenji A K Tanaka1, Shigekazu Kurihara2, Tetsuro Shibakusa3, Yasumasa Chiba4, Takashi Mikami5. 1. Institute for Innovation, Ajinomoto Co., Inc., Japan. Electronic address: kenji_tanaka@ajinomoto.com. 2. Institute for Innovation, Ajinomoto Co., Inc., Japan. Electronic address: shigekazu_kurihara@ajinomoto.com. 3. Institute for Innovation, Ajinomoto Co., Inc., Japan. Electronic address: tetsuro_shibakusa@ajinomoto.com. 4. Nutrition Care Department, Ajinomoto Co., Inc., Japan. Electronic address: yasumasa_chiba@ajinomoto.com. 5. Research Institute for Bioscience Products & Fine Chemicals, Ajinomoto Co., Inc., Japan. Electronic address: takashi_mikami@ajinomoto.com.
Abstract
BACKGROUND & AIMS: The control of inflammation is important for suppressing severe sepsis. Oral administration of cystine and theanine have been shown to suppress inflammatory responses due to invasion. Furthermore, the uptake of cystine into monocytes is promoted by exposure to lipopolysaccharide (LPS). In the present study, the effects of cystine were examined in the context of inflammatory responses. METHODS: Cystine was orally administered to mice, and the levels of interleukin (IL)-6 in the blood and spleen and the survival rates were calculated after the administration of LPS. The effects of cystine as well as neutralising anti-IL-10 antibodies on the LPS-induced production of IL-6 and IL-10 were examined in a monocyte cell line. RESULTS: The oral administration of cystine reduced IL-6 levels in the blood and spleen after LPS stimulation and improved survival rates. The addition of cystine to monocytes suppressed LPS-induced IL-6 production but enhanced IL-10 production. A neutralising anti-IL-10 antibody eliminated the inhibitory effects of cystine on the LPS-induced production of IL-6. CONCLUSIONS: The oral administration of cystine suppressed IL-6 production following LPS stimulation and improved survival rates in mice with LPS-induced sepsis. The enhanced production of IL-10 by monocytes may be involved in this anti-inflammatory response.
BACKGROUND & AIMS: The control of inflammation is important for suppressing severe sepsis. Oral administration of cystine and theanine have been shown to suppress inflammatory responses due to invasion. Furthermore, the uptake of cystine into monocytes is promoted by exposure to lipopolysaccharide (LPS). In the present study, the effects of cystine were examined in the context of inflammatory responses. METHODS:Cystine was orally administered to mice, and the levels of interleukin (IL)-6 in the blood and spleen and the survival rates were calculated after the administration of LPS. The effects of cystine as well as neutralising anti-IL-10 antibodies on the LPS-induced production of IL-6 and IL-10 were examined in a monocyte cell line. RESULTS: The oral administration of cystine reduced IL-6 levels in the blood and spleen after LPS stimulation and improved survival rates. The addition of cystine to monocytes suppressed LPS-induced IL-6 production but enhanced IL-10 production. A neutralising anti-IL-10 antibody eliminated the inhibitory effects of cystine on the LPS-induced production of IL-6. CONCLUSIONS: The oral administration of cystine suppressed IL-6 production following LPS stimulation and improved survival rates in mice with LPS-induced sepsis. The enhanced production of IL-10 by monocytes may be involved in this anti-inflammatory response.
Authors: Yenifer Gamarra; Felipe C Santiago; Jorge Molina-López; José Castaño; Lourdes Herrera-Quintana; Álvaro Domínguez; Elena Planells Journal: Crit Care Date: 2019-05-07 Impact factor: 9.097