Determine the equilibrium mechanical properties of articular cartilage from the short-term indentation response.

Indentation testing is widely used to evaluate the mechanical properties of articular cartilage. However, most curve-fitting solutions for indentation analysis require the deformation data of cartilage at the equilibrium state, which often takes the tissue hours to reach. The lengthy testing time reduces the efficiency of indentation, increases the chance of tissue deterioration, and prevents in vivo applications. To overcome these limitations, a novel technique based on principal component analysis (PCA) was developed in this study, which can predict the full indentation creep curve based on the first few minutes' deformation history and the principal components. The accuracy of this technique was confirmed using the indentation data from 40 temporomandibular joint condylar cartilage samples and 17 bovine knee joint samples. The mechanical properties determined by biphasic curve-fitting using predicted and experimental data are in good agreement, with the difference between the two less than 5%. For TMJ and knee cartilages, it is found that any number of full tests beyond eight will not lead to any increase larger than 1% in the accuracy, indicating a low sample number required for prediction. In addition, the principal components of indentation creep curves are consistent for the same type of cartilage tested with identical protocols, but significantly different between two distinct cartilages. Therefore PCA may also represent a new method to compare the mechanical behaviors of different cartilages, as it avoids the assumptions associated with mechanical constitutive models and relies purely on the experimental data.