Literature DB >> 2552772

Cycloheximide inhibits the induction of collagenase mRNA in chondrocytes exposed to synovial factors or recombinant interleukin-1.

C W Lin1, H I Georgescu, S L Phillips, C H Evans.   

Abstract

Synoviocytes secrete "chondrocyte activating factors" (CAF) which, like recombinant interleukin-1 (IL-1), induce the synthesis of collagenase by cultures of articular chondrocytes. Enzyme synthesis is preceded by the appearance of collagenase mRNA some 3-5 hours after exposure of the chondrocytes to CAF or IL-1. Cycloheximide (CX) inhibits the appearance of this message in a dose-dependent manner. At a concentration of 5 micrograms/ml inhibition by CX is completely reversible, with superinduction being observed in certain experiments. Identification of the newly synthesised proteins which are required for collagenase mRNA induction would greatly advance our understanding of collagenase gene expression.

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Year:  1989        PMID： 2552772     DOI： 10.1007/BF01972848

Source DB:  PubMed          Journal:  Agents Actions        ISSN： 0065-4299


  8 in total

1.  Induction of collagenase mRNA in lapine articular chondrocytes by synovial factors and interleukin-1.

Authors:  C W Lin; S L Phillips; C E Brinckerhoff; H I Georgescu; G Bandara; C H Evans
Journal:  Arch Biochem Biophys       Date:  1988-07       Impact factor: 4.013

2.  Platelet-derived growth factor-modulated translatable mRNAs.

Authors:  S L Hendrickson; C D Scher
Journal:  Mol Cell Biol       Date:  1983-08       Impact factor: 4.272

3.  Chondrocyte activation in response to factor(s) produced by a continuous line of lapine synovial fibroblasts.

Authors:  S Watanabe; H I Georgescu; D Mendelow; C H Evans
Journal:  Exp Cell Res       Date:  1986-11       Impact factor: 3.905

4.  Characterisation of chondrocyte activation in response to cytokines synthesised by a synovial cell line.

Authors:  K Sung; D Mendelow; H I Georgescu; C H Evans
Journal:  Biochim Biophys Acta       Date:  1988-09-16

5.  Interleukin-1 stimulates the secretion of proteoglycan- and collagen-degrading proteases by rabbit articular chondrocytes.

Authors:  R D Pasternak; S J Hubbs; R G Caccese; R L Marks; J M Conaty; G DiPasquale
Journal:  Clin Immunol Immunopathol       Date:  1986-12

6.  Isolation of a collagenase cDNA clone and measurement of changing collagenase mRNA levels during induction in rabbit synovial fibroblasts.

Authors:  R H Gross; L A Sheldon; C F Fletcher; C E Brinckerhoff
Journal:  Proc Natl Acad Sci U S A       Date:  1984-04       Impact factor: 11.205

7.  Pig catabolin is a form of interleukin 1. Cartilage and bone resorb, fibroblasts make prostaglandin and collagenase, and thymocyte proliferation is augmented in response to one protein.

Authors:  J Saklatvala; L M Pilsworth; S J Sarsfield; J Gavrilovic; J K Heath
Journal:  Biochem J       Date:  1984-12-01       Impact factor: 3.857

8.  Partial purification of a factor from human synovium that possesses interleukin 1, chondrocyte stimulating and catabolin-like activities.

Authors:  P R Elford; J E Meats; R M Sharrard; R G Russell
Journal:  FEBS Lett       Date:  1985-01-07       Impact factor: 4.124
  8 in total
  1 in total

1.  Cloning of the gene for interstitial collagenase-3 (matrix metalloproteinase-13) from rabbit synovial fibroblasts: differential expression with collagenase-1 (matrix metalloproteinase-1).

Authors:  M P Vincenti; C I Coon; J A Mengshol; S Yocum; P Mitchell; C E Brinckerhoff
Journal:  Biochem J       Date:  1998-04-01       Impact factor: 3.857
  1 in total

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