| Literature DB >> 25524880 |
Tri Budi Hartomo1, Thi Van Huyen Pham2, Nobuyuki Yamamoto2, Satoshi Hirase2, Daiichiro Hasegawa3, Yoshiyuki Kosaka3, Masafumi Matsuo4, Akira Hayakawa2, Yasuhiro Takeshima2, Kazumoto Iijima2, Hisahide Nishio1, Noriyuki Nishimura1.
Abstract
Despite the introduction of 13-cis-retinoic acid (13-cis-RA) into the current chemotherapy, more than half of high-risk neuroblastoma patients have experienced tumor relapses driven by chemoresistant cancer stem cells (CSCs) that can be isolated by their ability to grow as spheres. Although aldehyde dehydrogenase (ALDH) has been used to characterize CSCs in certain cancers, ALDH remains elusive in neuroblastoma. In the present study, we determined ALDH activity and expression of its 19 isoforms in spheres and parental cells of neuroblastoma. ALDH activity and several ALDH isoforms were consistently induced in spheres of different neuroblastoma cells. While ALDH1A2, ALDH1L1 and ALDH3B2 expression was consistently induced in spheres and associated with the sphere and colony formation, only ALDH1A2 expression was significantly correlated with the poor prognosis of neuroblastoma patients. ALDH1A2 expression was further associated with the growth and undifferentiation of neuroblastoma xenografts and the resistance of neuroblastoma cells to 13-cis-RA. These results suggest that ALDH1A2 is involved in the regulation of CSC properties in neuroblastoma.Entities:
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Year: 2014 PMID： 25524880 DOI： 10.3892/ijo.2014.2801
Source DB: PubMed Journal: Int J Oncol ISSN： 1019-6439 Impact factor: 5.650