S Thaler1, C Haritoglou2, F Schuettauf1, T Choragiewicz3, C A May4, F Gekeler1, M D Fischer1, H Langhals5, A Schatz1. 1. Eye Clinic and Institute for Ophthalmic Research, Centre for Ophthalmology, University of Tübingen, Tübingen, Germany. 2. Department of Ophthalmology, Ludwig-Maximilians-University, Munich, Germany. 3. 1] Eye Clinic and Institute for Ophthalmic Research, Centre for Ophthalmology, University of Tübingen, Tübingen, Germany [2] 1st Eye Hospital, Medical University of Lublin, Lublin, Poland. 4. Department of Anatomy, Medical Faculty 'Carl Gustav Carus', Technical University of Dresden, Dresden, Germany. 5. Department of Chemistry, Ludwig-Maximilians-University, Munich, Germany.
Abstract
PURPOSE: To investigate the biocompatibility of the new cyanine dye: 3,3'-Di-(4-sulfobutyl)-1,1,1',1'-tetramethyl-di-1H-benz[e]indocarbocyanine (DSS) as a vital dye for intraocular application in an in vivo rat model and to evaluate the effects of this dye on retinal structure and function. METHODS: DSS at a concentration of 0.5% was applied via intravitreal injections to adult Brown Norway rats with BSS serving as a control. Retinal toxicity was assessed 7 days later by means of retinal ganglion cell (RGC) counts, light microscopy, optical coherence tomography (OCT), and electroretinography (ERG). RESULTS: No significant decrease in RGC numbers was observed. No structural changes of the central retina were observed either in vivo (OCT) or under light microscopy. ERGs detected a temporary reduction of retinal function 7 days after injection; this was no longer evident 14 days after injection. CONCLUSIONS: DSS showed good biocompatibility in a well-established experimental in vivo setting and may be usable for intraocular surgery as an alternative to other cyanine dyes. In contrast to indocyanine green, it additionally offers fluorescence in the visual spectrum. Further studies with other animal models are needed before translation into clinical application.
PURPOSE: To investigate the biocompatibility of the new cyanine dye: 3,3'-Di-(4-sulfobutyl)-1,1,1',1'-tetramethyl-di-1H-benz[e]indocarbocyanine (DSS) as a vital dye for intraocular application in an in vivo rat model and to evaluate the effects of this dye on retinal structure and function. METHODS: DSS at a concentration of 0.5% was applied via intravitreal injections to adult Brown Norway rats with BSS serving as a control. Retinal toxicity was assessed 7 days later by means of retinal ganglion cell (RGC) counts, light microscopy, optical coherence tomography (OCT), and electroretinography (ERG). RESULTS: No significant decrease in RGC numbers was observed. No structural changes of the central retina were observed either in vivo (OCT) or under light microscopy. ERGs detected a temporary reduction of retinal function 7 days after injection; this was no longer evident 14 days after injection. CONCLUSIONS: DSS showed good biocompatibility in a well-established experimental in vivo setting and may be usable for intraocular surgery as an alternative to other cyanine dyes. In contrast to indocyanine green, it additionally offers fluorescence in the visual spectrum. Further studies with other animal models are needed before translation into clinical application.
Authors: Eric J Knott; Kristopher G Sheets; Yongdong Zhou; William C Gordon; Nicolas G Bazan Journal: Exp Eye Res Date: 2010-10-28 Impact factor: 3.467
Authors: Sebastian Thaler; Bogomil Voykov; Gabriel Willmann; Michal Fiedorowicz; Robert Rejdak; Florian Gekeler; C Albrecht May; Andreas Schatz; Frank Schuettauf Journal: Graefes Arch Clin Exp Ophthalmol Date: 2012-03-31 Impact factor: 3.117
Authors: Sebastian Thaler; Tomasz J Choragiewicz; Robert Rejdak; Michal Fiedorowicz; Waldemar A Turski; Maria Tulidowicz-Bielak; Eberhart Zrenner; Frank Schuettauf; Tomasz Zarnowski Journal: Graefes Arch Clin Exp Ophthalmol Date: 2010-06-08 Impact factor: 3.117