| Literature DB >> 25523173 |
Abhishek Ojha1, K Sowjanya Sree, Bindiya Sachdev, M A Rashmi, K C Ravi, P J Suresh, Komarlingam S Mohan, Raj K Bhatnagar.
Abstract
High survivorship of pink bollworrm, Pectinophora gossypiella in bolls of Bollgard® cotton hybrids and resistance to Cry1Ac protein, expressed in Bollgard cotton were reported in field-populations collected from the state of Gujarat (western India) in 2010. We have found Cry1Ac-resistance in pink bollworm populations sourced from Bollgard and non-Bt cotton fields in the adjoining states of Maharashtra and Madhya Pradesh in Central India. Further, we observed reduced binding of labeled Cry1Ac protein to receptors localized on the brush-border membrane of pink bollworm larval strains with high tolerance to Cry1Ac. These strains were sourced from Bollgard and conventional cotton fields. A pooled Cry1Ac-resistant strain, further selected on Cry1Ac diet also showed significantly reduced binding to Cry1Ac protein. The reduced binding of Cry1Ac to receptors could be an underlying mechanism for the observed resistance in pink bollworm populations feeding on Bollgard hybrids.Entities:
Keywords: BBMV, brush border membrane vesicle; Bollgard®; Bt, Bacillus thuringiensis; Cry1Ac-resistance; IRM, insect resistance management; MRC, Monsanto Research Center; NKJ, Nandurbar-Khargaon-Jalgaon; PBS, phosphate buffered saline; PBW, pink bollworm; brush border membrane vesicle, Bt cotton; pink bollworm; receptor binding
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Year: 2014 PMID： 25523173 PMCID： PMC5033187 DOI： 10.4161/21645698.2014.947800
Source DB: PubMed Journal: GM Crops Food ISSN： 2164-5698 Impact factor: 3.074