Guangliang Ding1, Tao Yan1, Jieli Chen1, Michael Chopp1, Lian Li1, Qingjiang Li1, Chengcheng Cui1, Ruizhuo Ning1, Quan Jiang2. 1. From the Department of Neurology, Henry Ford Hospital, Detroit, MI (G.D., T.Y., J.C., M.C., L.L., Q.L., C.C., R.N., Q.J.); and Department of Physics, Oakland University, Rochester, MI (M.C.). 2. From the Department of Neurology, Henry Ford Hospital, Detroit, MI (G.D., T.Y., J.C., M.C., L.L., Q.L., C.C., R.N., Q.J.); and Department of Physics, Oakland University, Rochester, MI (M.C.). qjiang1@hfhs.org.
Abstract
BACKGROUND AND PURPOSE: Diabetes mellitus is a disease with vascular components. Consequently, the blood-brain barrier disruption after stroke may differ between diabetic and nondiabetic animals. However, few studies have documented the longitudinal blood-brain barrier disruption afte stroke in diabetic animals. In this study, using MRI, we noninvasively evaluated the blood-brain barrier damage after middle cerebral artery occlusion in diabetic and nondiabetic rats. METHODS: Type 2 diabetes mellitus (T2DM) was induced in adult male Wistar rats by administration of a high-fat diet in combination with a single intraperitoneal injection (35 mg/kg) of streptozotocin. T2DM rats (n=9) and nondiabetic wild-type (WT) rats (n=9) were subjected to middle cerebral artery occlusion for 2 hours using the filament model. MRI was performed 1 day and then weekly for 5 weeks after middle cerebral artery occlusion for all rats. RESULTS: The ischemic lesion volumes after stroke as measured using T2 maps were not significantly different between the T2DM and WT rats. Compared with the WT rats, the volumes of blood-brain barrier disruption evaluated using contrast-enhanced T1-weighted imaging with gadolinium-diethylenetriamine penta-acetic acid and the cerebral hemorrhagic volumes measured with susceptibility-weighted imaging were significantly (P<0.05) larger in the T2DM rats from 1 to 5 weeks after stroke; values of diffusion fractional anisotropy were significantly lower in T2DM rats (P<0.03) than in WT rats after stroke. These MRI measurements were consistent with histological data. CONCLUSIONS: Using MRI, T2-weighted imaging did not detect significant differences of the ischemic lesion volumes between T2DM and WT rats. In contrast to the WT rats, however, contrast-enhanced T1-weighted imaging and susceptibility-weighted imaging identified much more severe ischemic vascular damage, whereas fractional anisotropy demonstrated lower axonal density in the T2DM rats after stroke.
BACKGROUND AND PURPOSE:Diabetes mellitus is a disease with vascular components. Consequently, the blood-brain barrier disruption after stroke may differ between diabetic and nondiabetic animals. However, few studies have documented the longitudinal blood-brain barrier disruption afte stroke in diabetic animals. In this study, using MRI, we noninvasively evaluated the blood-brain barrier damage after middle cerebral artery occlusion in diabetic and nondiabetic rats. METHODS:Type 2 diabetes mellitus (T2DM) was induced in adult male Wistar rats by administration of a high-fat diet in combination with a single intraperitoneal injection (35 mg/kg) of streptozotocin. T2DM rats (n=9) and nondiabetic wild-type (WT) rats (n=9) were subjected to middle cerebral artery occlusion for 2 hours using the filament model. MRI was performed 1 day and then weekly for 5 weeks after middle cerebral artery occlusion for all rats. RESULTS: The ischemic lesion volumes after stroke as measured using T2 maps were not significantly different between the T2DM and WT rats. Compared with the WT rats, the volumes of blood-brain barrier disruption evaluated using contrast-enhanced T1-weighted imaging with gadolinium-diethylenetriamine penta-acetic acid and the cerebral hemorrhagic volumes measured with susceptibility-weighted imaging were significantly (P<0.05) larger in the T2DM rats from 1 to 5 weeks after stroke; values of diffusion fractional anisotropy were significantly lower in T2DM rats (P<0.03) than in WT rats after stroke. These MRI measurements were consistent with histological data. CONCLUSIONS: Using MRI, T2-weighted imaging did not detect significant differences of the ischemic lesion volumes between T2DM and WT rats. In contrast to the WT rats, however, contrast-enhanced T1-weighted imaging and susceptibility-weighted imaging identified much more severe ischemic vascular damage, whereas fractional anisotropy demonstrated lower axonal density in the T2DM rats after stroke.
Authors: Quan Jiang; Changsheng Qu; Michael Chopp; Guang Liang Ding; Siamak P Nejad- Davarani; Joseph A Helpern; Jens H Jensen; Zheng Gang Zhang; Lian Li; Mei Lu; David Kaplan; Jiani Hu; Yimin Shen; Zhifeng Kou; Qingjiang Li; Shiyang Wang; Asim Mahmood Journal: NMR Biomed Date: 2011-03-23 Impact factor: 4.044
Authors: Guangliang Ding; Quan Jiang; Lian Li; Li Zhang; Zheng Gang Zhang; Karyn A Ledbetter; Lakshman Gollapalli; Swayamprava Panda; Qingjiang Li; James R Ewing; Michael Chopp Journal: Stroke Date: 2008-03-20 Impact factor: 7.914
Authors: Adviye Ergul; Mostafa M Elgebaly; Mary-Louise Middlemore; Weiguo Li; Hazem Elewa; Jeffrey A Switzer; Christiana Hall; Anna Kozak; Susan C Fagan Journal: BMC Neurol Date: 2007-10-15 Impact factor: 2.474
Authors: Weiguo Li; Rebecca Ward; John Paul Valenzuela; Guangkuo Dong; Susan C Fagan; Adviye Ergul Journal: Transl Stroke Res Date: 2017-03-01 Impact factor: 6.829
Authors: Guangliang Ding; Jieli Chen; Michael Chopp; Lian Li; Tao Yan; Qingjiang Li; Chengcheng Cui; Siamak P N Davarani; Quan Jiang Journal: PLoS One Date: 2016-02-22 Impact factor: 3.240
Authors: Ju Qiao; Christopher M Lawson; Kilian F G Rentrup; Praveen Kulkarni; Craig F Ferris Journal: J Transl Med Date: 2020-06-24 Impact factor: 5.531