Allison Hubel1,2, Ralf Spindler1,2, Julie M Curtsinger3, Bruce Lindgren4, Sara Wiederoder1,2, David H McKenna5. 1. Biopreservation Core Resource, University of Minnesota, Minneapolis, Minnesota. 2. Mechanical Engineering Department, University of Minnesota, Minneapolis, Minnesota. 3. Masonic Cancer Center Translational Therapy Laboratory, University of Minnesota, Minneapolis, Minnesota. 4. Biostatistics and Bioinformatics Core of the Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota. 5. Molecular & Cellular Therapeutics, University of Minnesota, Minneapolis, Minnesota.
Abstract
BACKGROUND: The continued growth in the uses of umbilical cord blood (UCB) will require the development of meaningful postthaw quality assays. This study examines both conventional and new measures for assessing UCB quality after long-term storage. STUDY DESIGN AND METHODS: The first arm of the study involved thawing UCB in storage for short (approx. 1 year) and long periods of time (>11 years). Conventional postthaw measures (colony-forming units [CFU], total nucleated cell counts, CD34+45+) were quantified in addition to apoptosis. The second arm of the study involved taking units stored in liquid nitrogen and imposing a storage lesion by storing the units in -80°C for various periods of time. After storage lesion, the units were thawed and assessed. RESULTS: In the first arm of the study, there was little difference in the postthaw measures between UCB stored for short and long periods of time. There was a slight increase in the percentage of CD34+45+ cells with time in storage and a reduction in the number of cells expressing apoptosis markers. When moved from liquid nitrogen to -80°C storage, the nucleated cell count varied little but there was a distinct decrease in frequency of CFUs and increase in percentage of cells expressing both early and late markers of apoptosis. CONCLUSION: Nucleated cell counts do not reflect damage to hematopoietic progenitors during long-term storage. Expression of caspases and other markers of apoptosis provide an early biomarker of damage during storage, which is consistent with other measures such as CFU and percentage of CD34+45+ cells.
BACKGROUND: The continued growth in the uses of umbilical cord blood (UCB) will require the development of meaningful postthaw quality assays. This study examines both conventional and new measures for assessing UCB quality after long-term storage. STUDY DESIGN AND METHODS: The first arm of the study involved thawing UCB in storage for short (approx. 1 year) and long periods of time (>11 years). Conventional postthaw measures (colony-forming units [CFU], total nucleated cell counts, CD34+45+) were quantified in addition to apoptosis. The second arm of the study involved taking units stored in liquid nitrogen and imposing a storage lesion by storing the units in -80°C for various periods of time. After storage lesion, the units were thawed and assessed. RESULTS: In the first arm of the study, there was little difference in the postthaw measures between UCB stored for short and long periods of time. There was a slight increase in the percentage of CD34+45+ cells with time in storage and a reduction in the number of cells expressing apoptosis markers. When moved from liquid nitrogen to -80°C storage, the nucleated cell count varied little but there was a distinct decrease in frequency of CFUs and increase in percentage of cells expressing both early and late markers of apoptosis. CONCLUSION: Nucleated cell counts do not reflect damage to hematopoietic progenitors during long-term storage. Expression of caspases and other markers of apoptosis provide an early biomarker of damage during storage, which is consistent with other measures such as CFU and percentage of CD34+45+ cells.
Authors: F de Boer; A M Dräger; H M Pinedo; F L Kessler; E van der Wall; A R Jonkhoff; J van der Lelie; P C Huijgens; G J Ossenkoppele; G J Schuurhuis Journal: Bone Marrow Transplant Date: 2002-02 Impact factor: 5.483
Authors: H S Goodwin; L M Grunzinger; D M Regan; K A McCormick; C E Johnson; D A Oliver; K A Mueckl; J M Alonso; D A Wall Journal: Cytotherapy Date: 2003 Impact factor: 5.414
Authors: Christopher Stroh; Uwe Cassens; Ajoy K Samraj; Walter Sibrowski; Klaus Schulze-Osthoff; Marek Los Journal: FASEB J Date: 2002-08-07 Impact factor: 5.191
Authors: Elisabeth E Spurr; Nicole E Wiggins; Katherine A Marsden; Raymond M Lowenthal; Scott J Ragg Journal: Cryobiology Date: 2002-06 Impact factor: 2.487
Authors: Kristin M Page; Lijun Zhang; Adam Mendizabal; Stephen Wease; Shelly Carter; Kevin Shoulars; Tracy Gentry; Andrew E Balber; Joanne Kurtzberg Journal: Transfusion Date: 2011-08-02 Impact factor: 3.157
Authors: Fransien de Boer; Angelika M Dräger; Herbert M Pinedo; Floortje L Kessler; M Monnee-van Muijen; Geert Weijers; Guus Westra; Elsken van der Wall; Tanja Netelenbos; Jan W Oberink; Peter C Huijgens; Gerrit J Schuurhuis Journal: J Hematother Stem Cell Res Date: 2002-12
Authors: John E Wagner; Juliet N Barker; Todd E DeFor; K Scott Baker; Bruce R Blazar; Cindy Eide; Anne Goldman; John Kersey; William Krivit; Margaret L MacMillan; Paul J Orchard; Charles Peters; Daniel J Weisdorf; Norma K C Ramsay; Stella M Davies Journal: Blood Date: 2002-09-01 Impact factor: 22.113
Authors: Alex L Chang; Richard S Hoehn; Peter Jernigan; Daniel Cox; Martin Schreiber; Timothy A Pritts Journal: Shock Date: 2016-09 Impact factor: 3.454
Authors: Soo Hyun Seo; Sue Shin; Eun Youn Roh; Eun Young Song; Sohee Oh; Byoung Jae Kim; Jong Hyun Yoon Journal: Ann Lab Med Date: 2017-03 Impact factor: 3.464