Ixodes ricinus and Its Transmitted Pathogens in Urban and Peri-Urban Areas in Europe: New Hazards and Relevance for Public Health.

Tick-borne diseases represent major public and animal health issues worldwide. Ixodes ricinus, primarily associated with deciduous and mixed forests, is the principal vector of causative agents of viral, bacterial, and protozoan zoonotic diseases in Europe. Recently, abundant tick populations have been observed in European urban green areas, which are of public health relevance due to the exposure of humans and domesticated animals to potentially infected ticks. In urban habitats, small and medium-sized mammals, birds, companion animals (dogs and cats), and larger mammals (roe deer and wild boar) play a role in maintenance of tick populations and as reservoirs of tick-borne pathogens. Presence of ticks infected with tick-borne encephalitis virus and high prevalence of ticks infected with Borrelia burgdorferi s.l., causing Lyme borreliosis, have been reported from urbanized areas in Europe. Emerging pathogens, including bacteria of the order Rickettsiales (Anaplasma phagocytophilum, "Candidatus Neoehrlichia mikurensis," Rickettsia helvetica, and R. monacensis), Borrelia miyamotoi, and protozoans (Babesia divergens, B. venatorum, and B. microti) have also been detected in urban tick populations. Understanding the ecology of ticks and their associations with hosts in a European urbanized environment is crucial to quantify parameters necessary for risk pre-assessment and identification of public health strategies for control and prevention of tick-borne diseases.

Introduction

Tick-borne infections are arthropod-borne diseases frequently reported worldwide. Ticks are known to transmit a great variety of pathogenic agents producing the highest number of human disease cases compared to other vector-borne diseases in Europe (1, 2). In general, the eco-epidemiology of zoonotic vector-borne diseases is very complex. It depends on the interactions of the vectors with the reservoir hosts and the pathogenic agents, which are modulated by several abiotic and biotic factors that vary in space and time. Certain tick-borne infections have recently been emerging in new regions or re-emerging within endemic sites and create an increasing concern for public health, food security, and biodiversity conservation (3–5). Global warming obviously affects the spread of tick-borne diseases, but climate alone does not determine the geographical distribution of tick species, their population densities and dynamics, the likelihood of their infection with microorganisms pathogenic for humans and animals, nor the frequency of contacts of humans and domestic animals with infected ticks (4, 6, 7). Socio-demographic factors, agricultural and wildlife management, deforestation and reforestation, are known to exert a big impact on the transformation of biotopes, thus affecting tick host assemblages as well as tick infection rates (8–10).

Urbanization as one of the socio-demographic factors has increased worldwide in recent decades (11, 12). Currently, more than half of the world’s population lives in urban areas, and it is expected that 70% will live in urban areas by 2050 (13). Nowadays, more than 75% of Earth’s ice-free lands show evidence of alteration as a result of human residence and land use, with less than a quarter remaining as wildlands. Europe shows the highest level of urbanization worldwide (14). Urbanization, due to restriction of natural areas, is known to dramatically change the composition of wildlife communities and affect the associated tick populations. In European cities, public parks, gardens, peri-urban leisure-time areas, and cemeteries became particularly important places where humans and domesticated animals can encounter potentially infected questing ticks (2).

Urban areas are highly fragmented environments composed of a mosaic of patches of various sizes, vegetation, and land-use types. Urban and peri-urban habitats are generally characterized by lower biodiversity of wildlife species compared to natural ecosystems. Urbanization often produces a certain gradient of homogenization in densely built-up areas, where synanthropic species adapted to urban habitats can be found and where species richness is reduced (15). On the other hand, suburban habitats are also occupied by native species comprising medium-sized mammalian predators and ground-foraging, omnivorous, and frugivorous birds that produce abundant populations there. But urbanization can also result in variation of animal species composition, e.g., by introduction of non-native species that replace native ones (16, 17).

Majority of the wildlife species commonly found in urban and peri-urban sites can serve as tick-maintenance hosts and also as reservoirs of tick-borne pathogens (18, 19). Furthermore, the majority of these species are generalists and are able to adapt to the urban and peri-urban environment and reach higher population densities than in natural sites (12, 20, 21). In urban habitats of Europe, rodents (mice, voles, dormice, squirrels, and rats), hedgehogs, shrews, birds, lizards, and companion animals (dogs and cats), but in peri-urban areas also medium-sized and larger mammals like foxes, roe deer, and wild boars, play the major role as tick-maintenance hosts and reservoirs of tick-borne pathogens (19, 22). Adaptation of wild animals to urban environment can also lead to increased contacts with humans and to increased risk of exposure to zoonotic agents. In addition, animal populations in urban areas can show genetic differentiation from wild populations of the same species. Thus urbanization can alter the biology and population densities of ticks and hosts and may lead to increased transmission of pathogens between vectors and urban-adapted hosts (11, 23). Moreover, urbanization is followed by increased mobility of humans, intensive long-distance trade, and new contacts of humans and companion animals with nature, which may contribute to changing of epidemiological and epizootiological conditions in urban and peri-urban areas (12) (Figure 1).

Figure 1

Complex factors of the biotic and abiotic environment influence the tick–host–pathogen interaction and consequently the occurrence of tick-borne diseases in urban and peri-urban environments.

Understanding the ecology of ticks and their association with various hosts in a changing urban and peri-urban European environment is therefore crucial to quantify various parameters necessary for the risk pre-assessment and for the identification of the best public health strategies for tick-borne disease management and prevention. The cascade of events including fluctuations in wildlife community composition and abundance, tick density and emergence, and spread of tick-borne pathogens in various habitat types in Europe are now being modeled as part of the EU FP7 project EDENext. In this review, we focus on Ixodes ricinus, one of the principal vectors of pathogens causing arthropod-borne infections in Europe, its associations with hosts and pathogens and risk of infection of humans in urbanized areas.

Ixodes ricinus – Vector of Multiple Pathogens

Ixodes ricinus (Acari: Ixodidae) is the most widespread tick species in Europe and transmits several viral, bacterial, and protozoan agents of medical and veterinary importance (8, 24–28).

The distribution area of I. ricinus has significantly expanded over the past decades. Recently, the species can be found in more northern areas and habitats at higher altitudes than a few decades ago (29–31). Increase in abundance, habitat expansion, including urbanized areas, and prolongation of the questing activity periods of I. ricinus, reported in recent years, are attributed to multiple and interacting factors (19, 26, 32). They include changes in land cover and land use due to alterations in agriculture and forestry management, changes in climate, changes in abundance, and distribution of wildlife due to altering wildlife management, and shifts in socioeconomic factors affecting the rate of exposure of humans to infected ticks (25, 26).

Risk factors associated with I. ricinus transmissible diseases can be divided generally into: (i) those directly related to climate change (acting on the tick, the host, or their habitats), (ii) those related to changes in the distribution of tick hosts (which may be a direct or indirect effect of human intervention), and (iii) other ecological changes (also commonly influenced by human intervention) (26).

Ixodes ricinus is primarily associated with shrubs and deciduous and mixed forests, with a high abundance of small, medium, and large wild vertebrate hosts. However, as a consequence of changing land use and wildlife management, persistent tick populations and high prevalences of infections with tick-borne pathogens have also been observed in urban and peri-urban sites in many European countries (33–41). Ixodes ricinus is a generalist exophilic tick species that is able to feed on over 300 different vertebrate species (42). It has a long-lasting life cycle, involving three active life stages (larvae, nymphs, and adults) that quest and attach to a host and feed on blood for a few days before detachment (parasitic life period) and subsequent molting or laying eggs (females). Each developmental stage requires its specific microhabitat comprising various biotic and abiotic factors. The parasitic on-host life of I. ricinus is limited to 3–5 days (larvae), 4–7 days (nymphs), and 7–11 days (females) of feeding on vertebrate hosts, whereas, the non-parasitic off-host life period of all developmental stages can last for several months or years (43). This extremely complex life cycle makes the tick vulnerable to alterations in habitat structure and availability of host animals.

In urban and peri-urban areas, the requirement for high relative humidity (above 80%) for extended periods of time by the off-host stages restricts the occurrence of I. ricinus to city parks with litter layers, forest patches, gardens, and cemeteries (22) where the continuous use of water to maintain the vegetation also increases the relative humidity. The other limiting biotic factor for I. ricinus in urban environments is the availability of medium-sized and large mammals as hosts of the adults, maintaining persistent and independent tick populations. Shifts in the tick–host associations to, e.g., hedgehogs, foxes, hares, domestic dogs, or cats, due to lack of large mammalian hosts can evoke changes in I. ricinus-borne pathogen spectrum, prevalence, and distribution. On the other hand, populations of large animals like deer and wild boar have become more abundant in large city parks and peri-urban areas around European cities, leading to the establishment of tick populations, shift of natural transmission cycles of some pathogens, and increase of the disease risk for humans and domestic animals (19).

Vertebrate Hosts of Ticks and Tick-Borne Pathogens in Urban Areas

Terrestrial vertebrate hosts are key players in the epidemiology of tick-borne diseases for at least two reasons. Firstly, they serve as maintenance hosts for ticks as a food resource and secondly, as reservoir hosts they are often responsible for the long-term maintenance of pathogens in both natural and urban habitats. Although many reports exist about the presence of pathogens in various hosts or ticks removed from them, the reservoir capacity for each of the pathogens in many cases remains to be experimentally defined. A reservoir host of tick-borne pathogens must fulfill certain criteria: (i) it must feed infected vector ticks, at least occasionally; (ii) it must take up a critical number of infectious agents during an infectious tick bite; (iii) it must allow the pathogen to multiply and to survive in at least certain parts of its body; and last but not least (iv) the pathogen has to find its way into other feeding ticks (44, 45). For this reason, the simple recording of pathogens (or nucleic acid of them) in a vertebrate host is not sufficient for classifying that host as a reservoir, but only a candidate reservoir when physiological and behavioral features may theoretically support pathogen amplification and transmission to the vector, or a simple carrier host, or a dead end host. Similarly, a higher prevalence in ticks removed from the vertebrate host compared to prevalence in questing ticks is only a good indication that the host is a candidate reservoir. However, to unambiguously prove the reservoir status of a host, xenodiagnostic experiments have to be carried out. They involve feeding of specific pathogen-free tick larvae from a laboratory colony on the tested host and the subsequent analysis of them for pathogens after their molt into the next stage. Unfortunately, for most pathogens and hosts, xenodiagnostic experiments have not been performed so far and the key hosts in the natural (and urban) cycle of tick-borne pathogens remain to be tested. The few exceptions are some species of mice, voles, rats, dormice, squirrels, and shrews (see details in Table 1) that had already been proven reservoirs of some tick-borne pathogens.

Table 1

Most important mammal hosts of .

Order	Species	Associated I. ricinus stage	Associated pathogens	Reference
Rodentia	Apodemus flavicollis	L, N	TBEV	(42, 46–50)
			Borrelia afzelii
			Borrelia burgdorferi s.s.
			Borrelia spielmanii
			Borrelia miyamotoi
			Cand. N. mikurensis
			Anaplasma phagocytophilum
			Babesia microti
	Apodemus sylvaticus	L, N	TBEV	(42, 46, 48–52)
			Borrelia afzelii
			Borrelia burgdorferi s.s.
			Borrelia spielmanii
			Cand. N. mikurensis
			Anaplasma phagocytophilum
			Babesia microti
	Apodemus agrarius	L, N	Borrelia afzelii	(42, 50, 53)
			Cand. N. mikurensis
			Anaplasma phagocytophilum
			Babesia microti
	Myodes glareolus	L, N	TBEV	(42, 48–50, 54, 55)
			Borrelia afzelii
			Borrelia burgdorferi s.s.
			Borrelia miyamotoi
			Cand. N. mikurensis
			Anaplasma phagocytophilum
			Babesia microti
	Microtus agrestris	L, N	TBEV	(42, 49–51, 56)
			Borrelia afzelii
			Babesia microti
			Cand. N. mikurensis
			Anaplasma phagocytophilum
	Microtus arvalis	L, N	Cand. N. mikurensis	(53, 55, 56)
			Anaplasma phagocytophilum
			Babesia microti
	Rattus norvegicus	L, N	Borrelia afzelii	(46, 57)
			Borrelia spielmanii
	Rattus rattus	L, N	Borrelia afzelii	(46, 50, 57)
			Anaplasma phagocytophilum
	Eliomys quercinus	L, N	Borrelia spielmanii	(46)
	Muscardinus avellanarius	L, N	Borrelia spielmanii	(58)
	Glis glis	L, N	TBEV	(42, 51)
			Borrelia afzelii
	Sciurus carolinensis	L, N	Borrelia afzelii	(42, 59)
			Borrelia burgdorferi s.s.
	Sciurus vulgaris	L, N	TBEV	(51, 60, 61)
			Borrelia burgdorferi s.s.
			Borrelia afzelii
			Borrelia garinii
	Eutamias sibiricus	L, N	Borrelia burgdorferi s.s.	(62)
			Borrelia afzelii
			Borrelia garinii
Lagomorpha	Lepus europaeus	L, N, A	Borrelia burgdorferi s.l.	(50, 63)
			Anaplasma phagocytophilum
	Lepus timidus	L, N, A	Borrelia burgdorferi s.l.	(63)
Soricomorpha	Sorex araneus	L, N	TBEV	(49–51, 63)
			Borrelia burgdorferi s.l.
			Anaplasma phagocytophilum
			Babesia microti
	Sorex minutus	L, N	Borrelia burgdorferi s.l.	(63)
Erinaceomorpha	Erinaceus europaeus	L, N, A	Borrelia afzelii	(64–68)
			Borrelia spielmanii
			Borrelia bavariensis
			Anaplasma phagocytophilum
	Erinaceus roumanicus	L, N, A	TBEV	(47, 64, 69)
			Borrelia afzelii
			Borrelia bavariensis
			Anaplasma phagocytophilum
			Cand. N. mikurensis
Artiodactyla	Capreolus capreolus	L, N, A	Anaplasma phagocytophilum	(70)
			Babesia venatorum
	Cervus elaphus	L, N, A	Anaplasma phagocytophilum	(71)
	Dama dama	L, N, A	Anaplasma phagocytophilum	(71)
Carnivora	Vulpes vulpes	L, N, A	Borrelia burgdorferi s.l.	(42, 72)
			Anaplasma phagocytophilum
	Meles meles	L, N, A	Borrelia afzelii	(73)
			Borrelia valaisiana

Mammal species that are experimentally proven reservoirs for pathogens are in .

Urban environments represent many special ecological characters in the complex communities of pathogens, ticks, and hosts. From a public and veterinary health perspective, city parks and peri-urban recreational areas are typical meeting places for humans (their pets) and ticks. Ticks in this respect serve as a bridge for pathogens, connecting reservoir hosts with humans. In addition to the frequent and likely encounter of humans with ticks, vertebrate host communities also differ substantially in many urban habitats compared to natural settings. Some important tick-maintenance and pathogen reservoir hosts (e.g., hedgehogs, squirrels, and songbirds) have no or very few natural enemies within urban environments, thus their populations might reach significantly higher densities compared to natural ones (21, 74). Besides the lack of predators, these urbanized vertebrates can also make use of man-made structures and anthropogenic food resources, like waste and pet food. Hedgehogs are one of the most successful urban adapters reaching up to nine times higher densities in urban areas than in rural areas (74). In Great Britain, red fox density was found at least 10-fold higher in cities than in rural areas (75, 76). The tendency to preserve green spaces inside cities is not only a positive aspect to humans but also for many tick-maintenance and reservoir hosts (12). For these urban dwellers, well established and dense shrubbery in parks offers shelter and nest sites. Furthermore, higher temperatures, especially during winter (heat island effect), are highly beneficial (74). All these factors can lead to an unbalanced vertebrate community that easily provides favorable ecological conditions for tick and pathogen cycles.

Mammals

Rodents are among the most important maintenance hosts for the subadult stages of I. ricinus (77). Furthermore, as pointed out by a recent analysis (78), ecologically widespread, synanthropic species with high density and fast life history such as rodents are often the most competent reservoirs for multi-host pathogens. As a consequence, mice and voles are also known to be important reservoirs for several pathogenic agents like tick-borne encephalitis virus (TBEV), Borrelia afzelii, and “Candidatus Neoehrlichia mikurensis” (Table 1). In addition to well-established rodent populations in cities, the frequent migration of these animals between human dwellings and natural environments can easily bring infected larvae and nymphs of I. ricinus into gardens and houses (79). Fluctuations in rodent densities are very important factors of disease risk (24, 80) with different ecological factors affecting rodent population dynamics in different parts of Europe. However, rodent population dynamics are less studied in urban and peri-urban parks than in natural areas. Rodents can harbor different endophilic (nidicolous) tick species (e.g., Ixodes trianguliceps and I. acuminatus). These do not pose a direct human hazard since they do not feed on humans. Their co-occurrence with I. ricinus on the same rodent, however, can lead to an exchange of pathogens among different tick species.

Little is known about the role of rats (Rattus rattus and R. norvegicus) in the urban maintenance of ticks and tick-borne pathogens. As one of the most efficient urban adapters, despite the control actions usually undertaken, they might be involved in the urban maintenance of Lyme borreliosis (LB) spirochetes (46, 57, 77). Other urbanized rodents, like garden dormice (Eliomys quercinus), hazel dormice (Muscardinus avellanarius) (46, 58) and hedgehogs (Erinaceus europaeus in Western Europe and E. roumanicus in Central and Eastern Europe) are also involved in the urban ecology of LB (Table 1). Hedgehogs have not only a longer life span compared to rodents but they also have the great advantage for ticks being able to feed not only larvae and nymphs but also a considerable number of adults (21, 81). Thus, they can easily maintain stable I. ricinus populations in urban areas in the long run (64).

In some cases, anthropogenic introduction of mammals into a new area can lead to the emergence of tick-borne pathogens even previously unknown for that region (12). The gray squirrel (Sciurus carolinensis) is native to North America, but an invasive species in the UK that has spread across the country and has largely displaced the native red squirrel (S. vulgaris). This species is a frequent urban dweller and can be an indirect source of human tick-borne infections since it has been experimentally shown to be reservoir for B. afzelii (59). Siberian chipmunks (Eutamias sibiricus) appeared as pets in many European countries but soon these rodents were recorded in urban parks of Rome (82, 83), Geneva (84), Brussels, and in and around many other towns (12). Chipmunks seem to be perfect hosts for subadult I. ricinus (85). Pisanu et al. (86) showed that these introduced rodents are more heavily infested by I. ricinus than native rodents such as the wood mouse (Apodemus sylvaticus) and the bank vole (Myodes glareolus). It was also found that the introduced rodent is associated with three species of B. burgdorferi sensu lato (s.l.), whereas, the native rodents are associated with only one species (62).

Lagomorphs (hares and rabbits) also inhabit anthropogenic landscapes and serve as blood sources for ticks (79). The European hare (Lepus europaeus) and the mountain hare (L. timidus) were shown to be not only effective tick-maintenance hosts but also reservoirs for B. burgdorferi s.l. (63). The European rabbit (Oryctolagus cuniculus) belongs to the most invasive mammalian species and its urban populations can harbor a variety of endo- and ectoparasites including I. ricinus (87). These lagomorphs can reach high densities and due to their ability to host adult I. ricinus as well, they are able to maintain an infective tick population even in urban and suburban areas where large mammals are not necessarily present. This double epidemiological function (tick-maintenance and reservoir host), which makes them key players in urban cycles of tick-borne pathogens is unique for lagomorphs and hedgehogs.

Bats can also carry different stages of I. ricinus ticks, thus they can also transport ticks to urban areas (88). Species especially adaptive in human dwellings, e.g., the lesser horseshoe bat (Rhinolophus hipposideros), can serve as tick-maintenance hosts but the role of these flying mammals in the pathogen life cycles remains to be clarified (54). Experimental TBEV viremia was shown in the greater mouse-eared bat (Myotis myotis), which is also a common urban inhabitant (51).

Among larger mammalian hosts, which can affect the circulation of tick-borne pathogens in peri-urban areas, roe deer (Capreolus capreolus), wild boar (Sus scrofa), and red foxes (Vulpes vulpes) are particularly important, because they can host all three active life stages of I. ricinus, and they increasingly live in urbanized areas (89, 90). Studies on roe deer abundance and movements can provide critical information for predicting tick dispersal and TBEV hazard (91, 92). Deer density is also suggested to be related to the LB incidence (31).

Tick density can be influenced by abundance and distribution of roe deer and red deer (Cervus elaphus) (93–95). Roe deer and red deer can inhabit a great variety of tick-infested habitats. Roe deer can even occur in some city parks, e.g., in Munich, Germany (70). Furthermore, the ability of deer to migrate more than 100 km carrying a high number of ticks is also known. This may facilitate the spreading of ticks to other areas (95, 96) and therefore potentially also of tick-borne pathogens from one area to another, although for some pathogen such as Borrelia spp., these species dilute the infection rate (97).

Wild boar populations have increased in Europe in recent decades and these animals are well adapted to live in urban and suburban forest areas (98). This species can provide a significant contribution to maintaining tick populations, although its role of reservoir of various tick-borne pathogens is only partially known (98, 99).

Foxes inhabit most urban areas across Europe and population increases have been seen in many European countries, e.g., in Great Britain and Switzerland (100, 101). In a recent study, I. ricinus was the most frequently detected tick on foxes in Germany, and all stages of this tick species were found on the animals (90). In Romania, I. ricinus infested almost 30% of foxes, indicating that these animals may play a significant role in the epidemiology of tick-borne diseases (102).

Urbanization largely concentrates humans in an area as well as a high number of pets (12). Among these, stray dogs represent an especially effective host for ticks, many of which are I. ricinus adults (103). They not only roam in large areas connecting natural and urban habitats, but they also get minimal or no treatment against ticks. Although we have limited knowledge on dogs’ role in the maintenance of tick-borne human pathogens (104–106), as effective hosts for I. ricinus adults they certainly contribute to the size of tick populations within gardens, parks, and sub-urban areas. The estimated 100 million free roaming dogs (owned and stray) living in Europe (107) certainly need to be taken into consideration during urban surveillance and control of ticks and tick-borne diseases.

Birds

Birds play an important role in the introduction of ticks and associated pathogens into urban areas (108, 109). Birds, especially ground-feeding song birds, are important maintenance hosts for larval and nymphal stages of I. ricinus. Common urban bird species foraging mostly on the ground and low shrub vegetation, such as common blackbird (Turdus merula), song thrush (Turdus philomelos), and European robin (Erythacus rubecula) were shown to be frequently infested with I. ricinus (110–112). More specifically, migratory birds have been shown to carry ticks and pathogens to large distances (113). However, the knowledge on the role of migratory birds in favoring the introduction of ticks and pathogens within new sites is so far very limited (114). Furthermore, earlier onset of spring migration and reproduction with more active ground-feeding activity of birds in the period of questing activities of I. ricinus larvae and nymphs may represent an additional risk factor for TBEV spread (115, 116). A recent study highlighted that migratory bird species were infested by more ticks than residents, with urbanized birds being the most parasitized (117). Thus in case of cities being close to bird resting or breeding sites (like cities and towns located on river banks) there is a realistic chance for the introduction and the maintenance of tick-borne pathogens (12). Birds as carriers of infected ticks probably play a role in the geographical spread of pathogens, such as Rickettsia helvetica, Anaplasma phagocytophilum, Babesia microti, and B. venatorum (118–120).

Lizards

Lizards have long been known as important hosts for ticks capable of feeding large amounts of immature I. ricinus (121) and they can often find suitable habitats in cities. In areas inhabited by lizards they can be as important tick-maintenance hosts as rodents (122, 123). Compared to rodents, however, lizards are more suitable hosts for nymphal I. ricinus (as shown by a lower larva/nymph ratio) (124–126). Sand lizards (Lacerta agilis), common wall lizards (Podarcis muralis), and green lizards (Lacerta viridis) are the most common species that can contribute to the urban maintenance of I. ricinus populations (122, 123, 125).

The role of lizards in the circulation of tick-borne pathogens has been underestimated compared to that of mammals and birds, but they have been proved to be reservoirs of LB spirochetes (122) and might also be involved in the life cycle of other tick-borne pathogens (124). However, experimental and field studies are needed to shed light on this epidemiological issue.

Pathogens Transmitted by Ixodes ricinus

Among the pathogens transmitted by I. ricinus, the western European TBEV subtype (TBEV-Eur), causing tick-borne encephalitis (TBE) (127) and spirochetes of the B. burgdorferi s.l. complex, the causative agents of human LB (128) have the greatest impact on human health. I. ricinus can also harbor bacteria of the order Rickettsiales that are of rising medical and veterinary importance. Among them, Anaplasma phagocytophilum can lead to granulocytic anaplasmosis in both humans and animals (50); the emerging pathogen “Candidatus Neoehrlichia mikurensis” can cause severe febrile illness in immunocompromised patients (129) and fever in humans without any primary disease (130); rickettsiae of the spotted fever group (SFG) (Rickettsia helvetica, R. monacenis) cause rickettsioses in humans (131). Protozoans of the genus Babesia, mainly B. divergens and B. microti, cause babesiosis in humans, and for B. venatorum pathogenicity to humans is suspected (132). The role of I. ricinus in transmission of Bartonella species (e.g., B. quintana and B. henselae) causing bartonellosis in humans is suspected (28, 133). Francisella tularensis, causing tularemia, and the Q fever agent Coxiella burnetii have also been detected in I. ricinus, but the role of this tick species in the epidemiology of these diseases is probably not significant (28, 133).

Tick-borne encephalitis virus

Tick-borne encephalitis is the most important tick-borne arboviral infection of humans in Europe and eastern and central Asia and is caused by the TBEV (Flaviviridae) (134–136). Ixodes ricinus is the principal vector for the western European (TBEV-Eur) subtype of the virus (127, 137). TBE is now endemic in 27 European countries (138) and its expansion northward and into higher altitudes has been observed in recent years (137, 139). There is a considerable lack of knowledge in the current fine scale spatial distribution of TBE, including urban areas, thus the risk of infection is still underestimated, especially considering that about two-thirds of human TBE infections are asymptomatic (135).

Incidence of TBE in Europe has been changing in a heterogeneous manner during the last decades, with spatial expansion in some areas and decrease in others (140–142). TBE ecology and epidemiology is expected to be affected considerably by climate change (143) and other drivers like changing in land-use patterns, expansion of forest coverage, increase of abandoned areas, and the creation of new suitable and fragmented landscapes for ticks and hosts within urban areas. Exposure to infected ticks is dependent on several and regionally variable socio-economical factors such as recreational and occupational human activities, public awareness, vaccination coverage, and tourism (26, 94, 144).

The majority of human TBE infections are acquired through bites of infected ticks, more rarely by the alimentary route through consumption of raw milk of infected goats, sheep, or cattle, or unpasteurized dairy products (145–147). As organic markets become more popular, city dwellers also have to be aware of the TBEV infection risk associated with unpasteurized cow and goat milk and milk products.

Tick-borne encephalitis incidence appears to be increasing, including urban areas, partially as a result of improvements in the diagnosis and reporting of TBE cases, but also due to increased exposure of humans to TBE due to outdoor activities. The risk of exposure to TBE was found to be relatively high even in the immediate surroundings of patients’ homes, e.g., in the Czech Republic (148), and an enhanced surveillance of TBE cases in Poland revealed that more than 50% of patients resided in urban areas (149).

Tick-borne encephalitis virus circulates mainly in natural sylvatic cycles involving vector ticks and reservoir hosts. However, due to expansion of urban sites to previously natural habitats and penetration of small and large wild animals into urban areas, reservoir hosts for TBEV as well as large tick-maintenance hosts can be present also in urban and peri-urban sites and thus ensure circulation of the virus there (150). Ticks remain infected throughout their life and it is suggested that they are not only vectors, but also long-term reservoirs of the virus (151). Rodents (A. flavicollis, A. sylvaticus, M. glareolus, and M. arvalis, see Table 1) are important reservoir hosts for TBEV-Eur (152, 153) and probably may maintain the virus in nature through latent persistent infection (154, 155). Co-feeding tick to tick transmission of TBEV, even in the absence of detectable viremia in these rodent species (156), is crucial to explain the focal distribution of the TBE foci and their potential variation over time (157). Experimental TBEV viremia has been demonstrated also in two lizard species (L. viridis and L. agilis) often occurring in urban areas (51), but field data on their reservoir competence for TBEV are missing. Migratory birds may play an important role in the geographic dispersal of TBEV-infected ticks, which can contribute to the emergence of new foci of disease, including gardens and urban parks, in case abiotic conditions and the vertebrate host spectrum are favorable for the maintenance of the pathogen (158). Among birds, thrushes (Turdus spp.) are the most frequently infested with I. ricinus ticks and also carry the most frequently infected ticks (159), however, the prevalence of TBEV-infected bird-feeding ticks is relatively low.

Wild and domestic ungulates, carnivores (foxes and dogs), and hares frequently occurring in peri-urban parks and forest patches within urbanized areas, are important actors in the dynamics of TBE, mainly as tick-maintenance hosts and carriers of infected ticks (160–162). Variation in abundance of roe deer was found to considerably affect TBE risk, depending on the threshold densities of tick, rodent, and large vertebrate populations in the area (31, 91, 92, 163). Ungulates probably do not contribute to the amplification of the virus, but may serve as sentinels to identify TBE foci (163, 164).

Accompanying dogs also represent an important risk factor for humans to acquire TBE. They are accidental hosts, but can become ill with TBE. In addition, during walking in natural forest or hunting activities, dogs come in contact with infected ticks and can carry them home or to urban parks, where they may later infest humans (165).

In general, data on TBEV prevalence in tick populations and seroprevalence in reservoir and sentinel hosts in urban areas and on the circulation of various virus strains in Europe are scarce (166–169). Furthermore, our knowledge on the mechanism favoring TBEV persistence and amplification in urban sites is very limited. TBEV infection rate in ticks is usually very low (<1%) (170–172), but can amount up to 15% in microfoci (173). TBEV-positive I. ricinus ticks have recently been detected, e.g., in a highly urbanized region in Southern Poland (estimated pool prevalence ranging from 0.19 to 1.11% for positive locations), suggesting the presence of active foci (174). TBEV-infected Dermacentor reticulatus adults were also detected in an urban area (Warsaw) in Poland, with higher prevalence (3.12%) than in natural areas. But our knowledge about the importance of this tick species in TBE epidemiology is still limited (175).

Generally, screening of ticks by PCR cannot be recommended for assessment of human TBE risk and alternative methods of environmental TBEV monitoring should be considered, such as serological long-term monitoring of rodents and other wild and domestic animals, which would serve as sentinel species (169).

Borrelia burgdorferi sensu lato

In little more than 30 years, Lyme borreliosis (LB), which is caused by the spirochete B. burgdorferi s.l., has risen from relative obscurity to become a global public health problem and a prototype of an emerging pathogen (176). During this period, we have accumulated enormous progress in knowledge of its phylogenetic diversity, molecular biology, genetics, host interactions, pathogenicity for humans as well as other vertebrate species, and preventive measures including vaccine development. But relatively little is known about public health consequences of LB in terms of eco-epidemiology issues and risk of acquiring infection in suburban and urban habitats.

Lyme borreliosis is the most abundant tick-borne disease of humans worldwide, though it only occurs in the northern hemisphere. LB occurs in North America (from the Mexican border in the south to the southern Canadian provinces in the north), the whole Europe, parts of North Africa (Maghreb), and northern Asia (Russian Siberia and the Far East, Sakhalin, Japan, China, and Korea). The geographical distribution of LB correlates closely with the range of the principal vectors, ticks of the I. ricinus complex (177). LB occurs between approximately 35° and 60°N in Europe, and between 30° and 55°N in North America. In countries at the southern limits of the LB range, its incidence decreases rapidly along the north-to-south gradient (178).

The B. burgdorferi s.l. complex now comprises up to 19 Borrelia species. Of these, only B. afzelii, B. burgdorferi, and B. garinii are proven agents of localized, disseminated, and chronic manifestations of LB in Europe, whereas, B. spielmanii has been detected in early skin disease, and B. bissettii and B. valaisiana have been detected in samples from single cases of LB (179, 180). The clinical role of B. lusitaniae remains to be substantiated.

Principal vectors of B. burgdorferi s.l. in Europe, including urban and suburban ecosystems, are two tick species: I. ricinus and I. persulcatus, the latter only occurring in eastern and north-eastern Europe. Moreover, the occurrence of I. hexagonus in the urban environment, due to the presence of suitable hosts, such as hedgehogs, cats, dogs, and foxes in gardens and public parks, could contribute to transmission of LB (65).

The risk of infection is particularly high in deciduous or mixed forest ecosystems or woodlands, along with city parks and urban gardens, especially gardens close to forests (181). The higher risk of contracting LB in the ecotones between forests and arable fields (178) or meadows, although higher densities of infected vector ticks are within forests, is an effect of frequent human presence along the edges of these habitats (182). Also forest fragmentation in suburban areas theoretically poses a greater risk due to enhanced proportion of ecotones (183). Other risks include reforestation (with increased population of forest rodents, but also deer, the principal host of adult vector ticks). For example, in the Czech Republic Zeman and Januska (184) found that LB risk correlated with overall population density of game (red deer, roe deer, mouflon, and wild boar) regardless of rodent abundance. Nevertheless, increased populations of reservoir hosts (forest rodents) usually stimulate the LB incidence.

All activities that increase human contact with ticks present risk for contracting LB, especially recreational (leisure time) activities in forested and urban areas (jogging, berry/mushroom picking, walking, and hiking), seasonal and occasional living by urban residents in country cottages, mowing and clearing of brush around the home in forested areas and gardening. Ownership of pet dogs and cats could also present a relative risk for humans when the pets are frequently parasitized by ticks and the owner tries to remove the ticks (178, 181). Moreover, outdoor employment and work (forestry workers, military personnel in the field, farmers, gardeners, gamekeepers, hunters, and rangers) are at risk. However, in most European countries, occupational exposure generally constitutes only 2% of LB cases (185), whereas, permanent residence in endemic areas with a high prevalence of infectious ticks (e.g., forested peri-urban areas) is a serious risk factor for LB.

Small rodents (A. sylvaticus, A. flavicollis, and M. glareolus) are regarded as the main reservoir hosts of LB pathogens in urban and suburban habitats across Europe (Table 1). Garden dormice (E. quercinus) (186) and hazel dormice (M. avellanarius) are especially competent reservoirs of the human pathogenic B. spielmanii (46, 58). Important role in the urban maintenance of B. spielmanii and B. afzelii could also be played by rats (R. norvegicus and R. rattus) (46, 57, 187). Other key urban players in the maintenance of LB spirochetes are hedgehogs (E. europaeus and E. roumanicus) (64, 65, 188). Red squirrels (S. vulgaris) were found to be heavily infested by ticks and feeding ticks showed high prevalence of infection in enzootic areas in Switzerland (60) and might consequently contribute to maintenance of spirochetes also in urban foci.

Dogs and cats are heavily infested with ticks and might act as hosts (probably not reservoirs) or sentinels for LB. The risk of exposure of dogs to numerous vector-borne pathogens has increased, and close relationship with humans in urban areas poses new concerns for human public health (106).

Ground-foraging bird species such as blackbird (T. merula), song thrush (T. philomelos), robin (E. rubecula), and pheasant (Phasianus colchicus) play a unique role in the epidemiology of LB and also contribute to the transmission cycle of B. burgdorferi s.l. in urban and suburban areas (189–192). Due to their specific immunity (complement system), certain bird species are resistant to some LB spirochetes but susceptible to others (193). They usually carry B. valaisiana and B. garinii and transmit these spirochetes to ticks. In 1998, two xenodiagnostic studies clearly defined the reservoir role of birds in the epidemiology of LB, one on a passerine bird, the blackbird (190), the other on a gallinaceous species, and the pheasant (194). However, the reservoir competence of other bird species needs to be clarified. A recent study showed that circulation of LB spirochetes is partly maintained by bird-specific tick species, and bridged by I. ricinus to other host types (195).

The role of lizards in the maintenance of B. burgdorferi s.l. is still controversial, since several lizard species have been shown to possess a complement with borreliacidal activity (196). However, in some areas LB spirochetes are more prevalent in sand lizards (L. agilis) and common wall lizards (P. muralis) than in rodents (122). The lizard-associated LB spirochete is B. lusitaniae, a genospecies previously thought to occur only in Mediterranean and Central Europe (197), but it was shown that it has a far more widespread geographical distribution involving the green lizard (L. viridis), the Balkan wall lizard (Podarcis taurica), and the sand lizard (L. agilis) (123, 125, 126).

We have reviewed the occurrence of B. burgdorferi s.l. in host-seeking urban I. ricinus ticks across Europe according to the literature (Table 2). There are also several additional papers demonstrating the presence of borreliae in ixodid ticks collected in (sub)urban areas (198–202). All accessible data show that borreliae in I. ricinus ticks collected in urban parks, gardens, or suburban habitats are prevalent approximately at the same rate as in I. ricinus ticks living in forests (203). In urban areas, therefore the risk of contacting LB could be as high as in natural environment.

Table 2

Occurrence of .

Country	City/region (habitat), year	No. of examined ticks	Prevalencea	Method	Genomic spp.	Reference
Czech Republic	Prague (U, S)	2,490 N, 143 F, 184 M	2–22%	IFA		(204)
	Prague (U, S) 1994–1997	12,287	3.3–13.3%	IFA		(205)
	Prague 1995–1997	462 N, 173 A	1.9% N, 12.7% A	PCR	Bg 18, Ba 13	(206)
	Brno – outskirts 1988	1,005	3.8% N, 16.4% F, 12.7% M	IFA		(207)
	Brno (U parks) 1992	34 N, 64 F, 65 M	14.7% N, 29.7% F, 30.8% M	DFM		(208)
	Brno-Pisárky (S) 1996–1998	643 N, 123 F, 107 M	10.0% N, 13.8% F, 18.7% M	DFM (and PCR)		(209)
	Brno-Pisárky (S) 2002	243 N, 19 F, 22 M	15.8% N + F + M	DFM (PCR)	Bg 15, Ba 14, Bb 2, Bv 2	(210)
Finland	Helsinki (U, S)	303 N, 189 F, 234 M	32.2% N + F + M	DFM, PCR, BSK	Ba 70%, Bg 25%	(35)
France	Paris (U, S)	360 N, 69 F, 129 M	32% F, 10% N, 20% M	PCR	Ba/Bv 36%, Bg/Bl 60%, Bm 4%*	(211)
Germany	Berlin – West (U, S)	1,414 N, 132 F, 165 M	2.4% N, 9.1% F, 6.1% M (MIR)	BSK		(212)
	Bonn (U, S) 2003	865 N, 241 F, 288 M	17.3% N, 26.6% F, 12.5% M	PCR	Ba 39%, Bg 28%, Bb 16%, Bv 9%	(36)
Hungary	Budapest (parks, forests, and cemeteries) 2013	240 F	40.8%	PCR		(213)
Italy	Imola (U parks) 2006		10.4% N + A	PCR		(214)
Lithuania	Vilnius (city park) 2005	39 A	25%	DFM, PCR	Ba, Bg, Ba + Bg	(215)
The Netherlands	Bijlmerweide (city park) 2000–2002	384 N + F + M	6.8%	PCR	Ba 10, Bb 1, Bv 1	(38)
Poland	Gdansk, Sopot, Gdynia (U, S)	701 N + F + M (164 F, 139 M)	12.4%, 11.6% F, 10.1% M	PCR		(216)
	Szczecin (U, S)	193 N, 22 A	17.7%	DFM		(217)
	Warsaw (U, S), 1996		19.2–31.0%	IFA (PCR)	Bg, Ba, Bv	(218)
	Warsaw (city parks)		6.1%	PCR		(219)
Serbia	Belgrade (U, S) 1996–2005	10,158 N + A	21.9% N + A	DFM (BSK, PCR)	Ba 75%, Bb 22%, Bg 3%	(220)
Slovakia	Bratislava (U, S) 1986–1988	77	7.8%	DFM		(221)
	Košice (U, S) 1991–1995	660 N, 2,904 A	9.2% N, 14.8% A	DFM and IFA		(222)
	Košice, Bardejov (U, S) 2008–2010	670	10.1%	PCR	Ba, Bg, Bv, Bb	(223)
Switzerland	Basel (U, S) 2003	172 N, 35 A	16.4% N + A	PCR		(224)
United Kingdom	London (U parks)	65 F	7.7% F	PCR		(225)

U, urban; S, suburban; .

.

* No sufficient discrimination between Bg and Bl and between Ba and Bv.

.

We should consider that most studies dealing with eco-epidemiology of LB in patients living in urban areas may have limitation, because not always the exact location (or area) where they acquired the vector tick is known. While popular opinion is that outdoor occupations and hiking are risk activities, several studies have implied that infection is often acquired near the home, during gardening and dog walking associated with increased risk (148, 226–228).

Anaplasma phagocytophilum

Anaplasma phagocytophilum is a small, gram-negative obligate intracellular alpha-Proteobacterium and infects neutrophilic, eosinophilic granulocytes, and monocytes of mammals. There, it replicates within a cytoplasmatic, cell-membrane derived vacuole. A. phagocytophilum is transmitted by ticks of the I. ricinus complex in the Northern hemisphere and in European countries mainly by I. ricinus (50).

The bacterium has been known since the last century to cause diseases in domestic ruminants (229) and since the 1960s in horses (230). The first human case was described in the USA in 1994 (231). The causative agents of the diseases were at the time classified into the granulocytic group of the genus Ehrlichia, which contained E. phagocytophila as agent of tick-borne fever of ruminants, E. equi as agent of equine granulocytic ehrlichiosis and the human granulocytic ehrlichiosis (HGE)-agent. In 2001, a reorganization of the order Rickettsiales, based on homologies in the 16S rRNA gene, reclassified the granulocytic Ehrlichia-group as the new bacterial species A. phagocytophilum and the respective diseases were then called granulocytic anaplasmosis (232). Clinical cases are also occurring in dogs and cats, then known as canine and feline granulocytic anaplasmosis (233, 234).

After the first cases appeared in the US in the 1990s, human granulocytic anaplasmosis (HGA) has become one of the most important tick-borne diseases in the US, with an incidence in 2010 of 6.1 cases per 1 million inhabitants. The first human case in Europe was described in the 1990s (235), and around 100 cases have been described since then in several European countries, e.g., in Slovenia, Croatia, Czech Republic, Slovakia, Austria, Latvia, the Netherlands, Norway, Poland, Spain, France, and Sweden (236–252). Seroprevalence rates in humans in Europe are around 1–20% and they fluctuate depending on anamnesis, tick exposure, and age of the patients (253).

Mammalian host species (Table 1) such as wild ruminants (e.g., roe deer, red deer, fallow deer, but also mountain ungulates), small mammals such as rodents and insectivores, but also foxes, bears, wild boars, birds, and reptiles are infected with A. phagocytophilum (50). Prevalence rates in wild ruminant species in Europe are generally high, e.g., ranging in roe deer and red deer from around 12% to over 85% (70, 254–256). On the other hand, prevalence rates in small mammals are from 0% to about 20% (50).

Anaplasma phagocytophilum is detected with varying prevalences in questing I. ricinus ticks, and has been found in Europe in nearly 30 countries. The prevalence ranged, for example, in Norway from 0.4 to 17.1%, in Estonia from 3 to 6.5%, in Slovakia from 1.1 to 8.3%, and in Germany from 1.0 to 17.4% [reviewed in Ref. (50)]. So far, transovarial transmission has not been shown in Ixodes ticks. As such, for the current state of knowledge, a reservoir host is necessary to keep up the endemic life cycle of A. phagocytophilum in nature.

The discrepancy of a high occurrence of A. phagocytophilum in ticks and mammals as well as high seroprevalence rates in Europe in contrast to few clinical cases has been explained by the potential underdiagnosing of the disease, or the potential occurrence of less virulent strains in Europe in comparison to the USA. The discrepancy could also be explained by a higher awareness of US physicians to the disease because in the USA it is a notifiable disease. However, A. phagocytophilum shows also genetic heterogeneity and potential differences concerning the potential host tropisms and pathogenicity (118). A potential human pathogenic strain of A. phagocytophilum in Europe has been especially suspected to be connected with wild boars. This was confirmed in recent studies (257, 258).

Several studies have investigated the genetic heterogeneity on the basis of several genes such as 16S rRNA, groEL heat-shock protein, major surface protein coding genes, and the ankA gene (255, 259–261). Several distinct clusters were found where, in general, strains derived from domestic animals or ruminants clustered together. Roe deer strains often clustered separately from strains derived from other animals. No evidence was found that wild ruminants are involved in the transmission cycles of potentially pathogenic strains. This was shown again by a recent multi locus sequence typing study (262). However, another study found pathogenic strains associated mostly to ungulates (118).

Furthermore, in a recent large-scale analysis, four A. phagocytophilum ecotypes with significantly different host ranges were identified based on groEL heat-shock protein gene sequences of various European vertebrate and tick samples (99). So far, all human cases clustered in ecotype I with the broadest host range (including domesticated animals, red deer, wild boar, and urban hedgehogs). Ecotype II was associated with roe deer and some rodents, ecotype III included only rodents. Birds seem to have a different enzootic cycle from all these (ecotype IV). Based on population genetic parameters, ecotype I showed significant expansion, which might have occurred through an increase in either the population of I. ricinus ticks, or in the (often urban) vertebrate host species, or in both (99).

Only recently, a HGA case of a German patient has been published having acquired the infection whilst on holidays hiking in Scotland (263). This shows that the risk of contracting this infectious agent can also be in leisure time whilst hiking, or even in the cities whilst being in urban or peri-urban park areas.

In about the last 5 years, considerable research effort has been undertaken in Europe to investigate the epidemiology of A. phagocytophilum, especially in urban areas and high prevalences of this pathogen have been found with seasonal and geographic variability. An overview of recent studies investigating questing I. ricinus in urban and suburban areas is shown in Table 3. However, when considering A. phagocytophilum prevalence rates in ticks, the genetic variability has to be taken into account as not all strains may be pathogenic to humans.

Table 3

Occurrence of .

Country	City/region (habitat)	No. of ticks posit./examined	Prevalenceb (%)	Reference
Austria	Graz (RA)	5/518	1	(264)
Czech Republic	Dvur Kralove (U forest)	8/138	5.8	(265)
	Ostrava (U park)	276 (tested in pools)	9.4	(266)
France	Paris (S forests)	2/558	0.7	(211)
Germany	Hamburg (U RA)	51/1,400	3.6	(267)
	Hannover (U RA)	94/2,100	4.5	(268)
	Bavaria (U parks)	500/5,569	9.0	(269)
	Bavaria (U parks)	103/2,862	2.9	(270)
	Bavaria (U parks)	172/2,800	6.1	(271)
	Leipzig (U, S RA)	47/539	8.7	(55)
	Hannover (U RA)	52/1,646	3.2	(272)
Hungary	Budapest (30 sites: U parks, forests, and cemeteries)	21/240	8.8	(213)
Poland	S forests	18/124; 6/46	14.5; 13.0	(273)
Slovakia	Bratislava (U, S forests)	10/248	4	(265)
	Malacky (U park)	4/101	4	(265)
	Košice (U forest)	10/224	4.5	(265)
	Bardejov Poštárka (S forest)	2/75	2.7	(40)
	Košice Adlerova (S forest)	10/261	3.8	(40)
	Jazero (U forest)	5/91	5.5	(40)
	Košice (S forests)	1,075	1.4–5.5	(274)

U, urban; S, suburban; RA, recreational area.

,

.

,

.

Candidatus Neoehrlichia mikurensis

“Candidatus Neoehrlichia mikurensis” (Candidatus N. mikurensis) is a tick-borne pathogen, which is probably transmitted by I. ricinus ticks (24). However, transovarial transmission in this tick species has not been reported yet.

Currently, the genera Wolbachia, Ehrlichia, Neorickettsia, Aegyptianella, and Anaplasma belong to the rickettsial family Anaplasmataceae (232). Most certainly, the new genus “Neoehrlichia” will be included in this family in future. The pathogens of this family are intracellular bacteria transmitted by arthropods and may cause severe diseases in humans and animals. For at least three of the five existing genera within this family (Anaplasma, Ehrlichia, and Neorickettsia) serological cross reactions are not known so far (275). Candidatus N. mikurensis is an obligate intracellular gram-negative bacterium, which is characterized by an endothelial cell tropism but it could not be cultivated in vitro thus far. Therefore, the status “Candidatus” is still preserved.

A previous study published data on not taxonomically grouped Ehrlichia DNA in engorged I. ricinus ticks from roe deer in the Netherlands (276). This pathogen was then named after the senior author as “Schotti-Variant” (276). Similar sequencing results were published for I. ricinus and I. persulcatus ticks from the Baltics in 2001 (277). Between 1998 and 2001, DNA of a pathogen, suggested to be called Cand. Ehrlichia walkerii spp. nov., was found in engorged I. ricinus ticks that fed on asymptomatic patients from Italy (278). In 2003, DNA sequences of this new pathogen were detected in DNA extracted from I. ricinus ticks from Germany, followed by first investigations on possible reservoir hosts (279). In 2003, a pathogen was found via examination by conventional PCR in three wild rats (R. norvegicus) in China. This examination was followed by DNA sequencing of this pathogen, which was then called the “Rattus Variant” (280). In 2004, DNA of this “new” pathogen was found in 7 out of 15 brown rats from a Japanese isle called Mikura (275). The pathogen was passaged in Wistar rats and first investigations on the ultrastructure and the phylogenetic analysis were done, which lead to the currently valid taxonomic denomination “Candidatus Neoehrlichia mikurensis.” The close genetic similarity of the 16S rRNA and the groEL gene puts Candidatus N. mikurensis in the family of Anaplasmataceae.

Candidatus N. mikurensis was found widespread in I. ricinus throughout Europe (281, 282). It could be detected in Italy, France, Sweden, Russia, and other European countries (Table 4). The prevalences ranged between 1 and 11% but focal areas were found with prevalence rates up to 26.6% (49) (Table 4). Furthermore, Candidatus N. mikurensis was detected in one out of 126 I. ricinus ticks that were collected in Moldavia back in the year 1969 (283) and it was only detected in the genus of Ixodes ticks so far (284). Positive ticks were not only found in sylvatic and non-anthropogenic sites but also in urban and peri-urban sites with human influence in Europe (Table 4).

Table 4

Occurrence of .

Country	No. of sites, habitat	No. of ticks examined	Prevalencea	Reference
Austria	U, S, 2002–2003	518	4.2%	(264)
Czech Republic	U, 2010	69	0.4%	(265)
Denmark	Three sites, S, sylvatic, 2011(+tick DNA from archive)	79a	3.8%	(285)
France	Two sites, sylvatic	60	1.7%	(282)
Germany	Ten sites, U, S	542	8.1%	(282)
	U, S, 2008–2009	782	24.2–26.6%	(52)
Hungary	Nine sites, 2007	2,004	n.a. 9 of 35 sites positive	(286)
Italy	U, S, 2006–2008	138	10.5%	(287)
The Netherlands	Three sites, sylvatic	180	8.6%	(288)
	Twenty-one sites, U, S, sylvatic, 2006–2010	5,343	5.6%	(289)
The Netherlands/Belgium	n. a., 2006–2010	2,375	7%	(281)
Russia	S, sylvatic, 1997–1998	295	7.1%	(277)
Slovakia	S, sylvatic, 2006	68	2.9%	(290)
	Ten sites, U, S, sylvatic, 2008, 2010	670	2.4%	(40)
	U, S		1.1–4.5%	(265)
Spain	S, 2013	100	2%	(291)
Sweden	Four sites, sylvatic, 2010–2011	949	4.5–11%	(292)
Switzerland	Eleven sites, U, S, 2009–2010	818	6.4%	(293)
	Four sites, U, S, 2009	1,916	3.5–8%	(294)

U, urban; S, suburban.

.

.

Previous studies on potential reservoir hosts revealed that rodents, especially bank voles and yellow-necked mice, but also common voles (M. arvalis) were infected at high rates, suggesting a role as reservoir hosts (52, 281, 295, 296), but insectivores were found to be negative for Candidatus N. mikurensis thus far (52). Recently, the reservoir role of Apodemus mice (A. flavicollis and A. sylvaticus) and bank voles (M. glareolus) has unambiguously been proven in a xenodiagnostic study [(48); Table 1]. Urban hedgehogs (E. roumanicus) with high density in a Budapest city park were found to be carriers of Candidatus N. mikurensis, indicating that non-rodent reservoirs might be also involved in the maintenance of this pathogen, especially in human dwellings (69). Additionally, Candidatus N. mikurensis was detected in dogs from Germany and Nigeria (297, 298).

In the past, the detection of Candidatus N. mikurensis in rodents and ixodid ticks was an interesting but only incidental finding without any medical importance (299). In contrast to this assumption, it was recently found in humans (50) with immune deficiency but without being in an occupation group at risk for tick bites over the last decade. Candidatus N. mikurensis caused unspecific symptoms such as fever, septicemia, malaise, and weight loss in these patients (300–302). Until October 2012, the first six clinical cases of neoehrlichiosis were the only human cases confirmed by laboratory diagnostic methods. All of these patients suffered from a primary disease, were immunocompromised and came from European countries, such as Germany (301), the Czech Republic (303), Sweden (302), and Switzerland (300). Nevertheless a primary disease is not a necessary precondition to develop neoehrlichiosis as Candidatus N. mikurensis could be detected in blood of 7 out of 622 patients from China suffering from fever (130). The authors of these clinical reports emphasize that these seven patients were otherwise healthy and did not suffer from a chronic or immunosuppressive disease. The most recent two human cases were reported in Switzerland, where both patients recovered quickly after a treatment with Doxycycline (294). The data, gained in the last decade, lead to the assumption that Candidatus N. mikurensis is an emerging pathogen that might be found by increasing numbers in ticks from sylvatic and urban sites, in small mammals and humans in future (281, 304). Further investigations are needed on the spread, maintenance, and potential reservoir hosts to assess the risk potential of Candidatus N. mikurensis.

Rickettsiae

Rickettsiae are Gram-negative, obligate, aerobic, intracellular bacterial parasites of eukaryotes that survive freely within the cytosol of the host cell, and belong to the family Rickettsiaceae and order Rickettsiales. Rickettsiae are traditionally subdivided into the typhus and the spotted fever group (SFG). SFG rickettsiae are associated with hard ticks (Ixodidae), with the exception of Rickettsia akari (mite-borne) and R. felis (flea-borne). Hard ticks can transmit them transstadially and transovarially and serve both as vectors and reservoirs of these pathogens. Vertebrates are suspected to serve as reservoirs of rickettsiae, but they may also be accidental hosts and acquire infection by a tick bite (305). However, in a recent xenodiagnostic experiment infected rodents were not able to transmit R. helvetica or R. monacensis to I. ricinus larvae (48).

In Europe, R. felis, R. typhi, R. prowazekii, R. akari, R. conorii, R. slovaca, R. sibirica mongolotimonae, R. raoultii, R. massiliae, R. aeschlimanni, R. helvetica, and R. monacensis have been implicated in human diseases or reported as emerging pathogens or isolated from vectors or humans (131, 306–308). Furthermore, the candidate species “Candidatus Rickettsia kotlanii,” “Candidatus Rickettsia barbariae,” or “Candidatus Rickettsia vini” have been found in ticks in Europe (309–311). Numerous rickettsiae are regularly associated with ticks and have been called symbionts, microsymbionts, or endosymbionts (living in endocellular symbiosis). However, their potential for pathogenicity is still unknown (312).

The presence of tick-borne rickettsiae has been reported from almost all European countries. The current view on geographic distribution of Rickettsia species in the world is summarized by Parola et al. (131).

In Europe, I. ricinus ticks are known to carry mainly R. helvetica and R. monacensis. However, R. massiliae was also detected in I. ricinus ticks (313). The following rickettsial genotypes were detected only by molecular tools in I. ricinus ticks collected in Europe: “Candidatus R. vini” was proposed as a new Rickettsia spp. detected in I. arboricola and I. ricinus collected from three different bird species in Spain (311), Rickettsia spp. strain Davousti, previously found in Amblyomma tholloni ticks in Africa, was detected in Ixodes spp. collected from migratory birds in Sweden (314),“Candidatus Rickettsia moreli” (GenBank accession numbers Y08784 and Y08785) was detected in I. ricinus from Spain, and Rickettsia spp. clone KVH-02-3H7 (GenBank accession number GQ849216) was detected in I. ricinus in the Netherlands (131).

Rickettsia helvetica was first isolated from I. ricinus in Switzerland and it was confirmed to be a new member of the SFG rickettsiae in 1993 (315, 316). It has been generally accepted that I. ricinus is the main vector and natural reservoir of R. helvetica. However, D. reticulatus ticks were found to be infected with R. helvetica in Croatia (317). R. helvetica has been detected in questing and bird-feeding I. ricinus ticks in at least 24 European countries (131). The prevalence rates vary from 0.5% in a bird conservation island named Greifswalder Oie in the Baltic Sea to 66% in the Netherlands (318, 319). For example, the highest infection rate of R. helvetica in I. ricinus from Denmark was found in May, followed by July, August, and October (320). The presence of R. helvetica was also confirmed in I. ricinus in some urban and peri-urban sites in Slovakia, the Czech Republic, Germany, Portugal, Serbia, and Poland (Table 5).

Table 5

Occurrence of .

Country	City/region (habitat)	No. examined ticks	Prevalence of Rickettsia spp.	Identified species (n)	Reference
Czech Republic	Ostrava (U park), 2010	180 N	2.2% (MIR)	14 Rh, 6 Rm	(266)
		96 A	4.2% (MIR)
	Proskovice (mixed forest), 2010	1,114 N	3.5% (MIR)
		83 A	2.5% (MIR)
France	Paris (S)	360 N, 69 F, 129 M	5.8%	Rh	(211)
Germany	Munich, 2006	961 N	1.0%	138 Rh, 13 Rm	(321)
		1,900 A	7.3%
	Saarland (RA), 2008–2009	36 N	16.7–47.2%	8 Rh	(322)
	Bavaria/Munich (natural alluvial forest), 2008–2009	79 A	21.5%
	Leipzig/Saxony (coal surface-mining area), 2008–2009	28 N	21.4%
		100 A	19.0%
		98 N	8.2–27.6%
		431 A	9.7%
	Munich, Regensburg, Ingolstadt, Augsburg, Berg (U parks), 2009–2010	774 L	2.1–9.8%	15 Rh, 1 Rm	(37)
		1,190 N	6.8%
		2,495 A	7.5%	77 Rh, 4 Rm
		244 L	–
		742 N	–	180 Rh, 8 Rm
		1,142 A	–
	Munich, Regensburg, Lake Starnberg (U, S)	24 L	2.2–7.5%	29 Rh,1 Rm	(323)
	Lake Starnberg and Lake Ammersee, pastures	500 N	5.0%
	Augsburg, forest, 2011	889 A	8.7%
		140 N	15.7%	9 Rh
		225A	13.3%
		139 L	2.2–10.1%
		120 N	17.5%	9 Rh
		79 A	13.9%
	Hanover (U park), 2010	31 L	16.0%	268 Rh	(268)
		1,697 N	25.5%
		372 A	30.4%
Poland	Warsaw, national parks and natural areas, 2011	1,147 N 442 A	3.7% (MIR)	38 Rh, Rm	(41)
			5.9% (MIR)
Portugal	Alentejo (safari park), 2006–2009	35 A	82.9%	14 Rh, 15 Rm	(324)
Serbia	Four natural sites, 2 sites (RA), 2007, 2009	26	23.1%	2 Rh, 4 Rm	(325)
Slovakia	Bratislava (S forest, cemeteries), 2006–2011	445 N	8.3%	61 Rh, 3 Rm	(326)
		471 A	10.2%
	Malacky (U park), 2006–2011	59 N	6.8%	10 Rh, 3 Rm
		62 A	14.5%
	Martin (U park), 2006–2011	3 N	0
		12 A	16.7%
	Martinské hole Mts (mountain forest), 2006–2011	276 N	5.4%	6 Rh, 2 Rm
		482 A	10.0%
	Vojka nad Dunajom (RA), 2011–2012	2 N	0	30 Rh, 3 Rm	(327)
		280 A	11.7%

U, urban; S, suburban; RA, recreational area; .

In 1999, R. helvetica was associated with chronic perimyocarditis in sudden cardiac death in Sweden (328). This species has been cultivated from a patient with subacute meningitis (329). The hypothetical role of R. helvetica as an etiological agent of sarcoidosis could not be confirmed (330). The illness is associated with fever, headache, arthralgia, and myalgias and less frequently with rash and/or an eschar (331, 332).

Rickettsia monacensis was originally isolated as new species from I. ricinus collected in a city park in Germany (333). Phylogenetic analyses of the 16S rRNA, gltA, and rompA gene sequences demonstrated its close relationship with Candidatus Rickettsia spp. IRS3 and Cand. Rickettsia sp. IRS4 isolated from I. ricinus in north-eastern and south-western Slovakia (334, 335). The prevalence rates of R. monacensis in I. ricinus ticks vary from 0.5% in Germany to 34.6% in Turkey (322, 336). R. monacensis has been detected in I. ricinus ticks in at least 18 European countries (131). The presence of R. monacensis was also confirmed in I. ricinus ticks in some urban and peri-urban sites in Slovakia, the Czech Republic, Germany, Portugal, Serbia, and Poland (Table 5). In 2005, R. monacensis was identified as a human pathogen in two patients in Spain (in June and September) and latter in one patient in Sardinia, Italy (in April) (337, 338). In addition to fever and flu-like symptoms, the inoculation eschar was identified in an Italian patient, and a generalized rash including the palms and soles was identified in a Spanish patient.

Rickettsia massiliae was originally isolated from Rhipicephalus sanguineus ticks collected near Marseille, France, in 1992 and then detected in R. sanguineus, R. turanicus, R. pusillus, R. bursa, and I. ricinus ticks in France, Greece, Portugal, Switzerland, Spain, including islands: Sardinia and Sicily (Italy), the Canary Islands (Spain), Cephalonia (Greece), and Cyprus (131). R. massiliae was identified in four I. ricinus ticks removed from humans at hospitals in Castilla y León, Spain (313). However, to our knowledge, there are no other studies of this species in urban areas.

Babesia

Ixodes ricinus is the vector of three intraerythrocytic protozoan parasites circulating in Europe and involved in human babesiosis: B. divergens, B. venatorum (originally designated Babesia spp. EU1), and B. microti. To date, no other Piroplasmida affecting humans have been reported to be transmitted by this tick species, even though it feeds on a very large spectrum of hosts, which are potentially infected by several parasite species including numerous other Babesia species associated to wildlife or domestic animal diseases. However, the list of potential or known tick-borne pathogens is constantly evolving, either due to: (i) the description of Babesia species new for science, (ii) the spread of parasite species previously unknown in Europe, or (iii) the discovery of a Babesia species previously restricted to animals but now known to be associated with humans. Thus, emergence or re-emergence of tick-borne diseases leads to the development of unknown health risks (339). Therefore, there is a real concern that tick-borne diseases due to parasites will appear in areas previously free of such diseases, and there is a real necessity of an epidemiological surveillance of the parasitic communities hosted, and potentially transmitted by ticks (340).

Although best known as an animal disease, babesiosis is a zoonotic disease, classified as emerging by some authors. Approximately 50 human cases of babesiosis have been reported in Europe, which is probably underestimated because of a large proportion of asymptomatic infections, as suggested by seroprevalence studies (341). Among the Babesia species pathogenic for humans, the bovine parasite B. divergens is thought to be responsible for most European cases of human babesiosis (342). However, since 2003, cases of human babesiosis have also been attributed to B. venatorum in Austria, Italy, and Germany (343, 344) as well as to B. microti in a single case in Germany (341). Whilst the clinical signs of human babesiosis are usually limited to splenectomized patients, two human cases (one attributed to B. divergens, the other to an unknown origin) have been detected in immunocompetent patients in eastern France (345). It is also noticeable that, as an example, 0.38% of the French population is splenectomized (346). Moreover, the rising number of HIV-positive individuals and the increasing population of immunocompromised humans, especially in urban areas, may therefore lead to boost the number of human babesiosis cases (341). The proportion of the population at risk of Babesia infection is thus higher than previously suspected and Babesia spp. likely represents real potential agents of an emerging zoonotic disease and needs increased attention and vigilance.

Besides transstadial transmission, transovarial transmission within ticks is characteristic for most Babesia spp. (differentiating them from Theileria species), which implies that ticks constitute a real parasite reservoir in the field, facilitating the long-term persistence of Babesia species in the ecosystem (sometimes over several tick generations) (347). In Europe, infection rates of Babesia spp. in ticks are usually rather low, but published values range from 0.9 to 20% (341).

Babesia divergens is a bovine parasite transmitted by I. ricinus, and is thought to be responsible for most cases of human babesiosis in Europe (342). This parasite is the most widespread and pathogenic Babesia species infecting cattle in northern temperate areas (342). Thus, any urban or peri-urban area where cattle and I. ricinus are found is potentially at risk. For example, B. divergens has been found in an I. ricinus tick collected in an urban park in Germany (37). Recently, the discovery of this parasite in questing I. ricinus from a forest area in Eastern France (340), as well as in I. ricinus collected from wild cervids in Belgium (348), may suggest that its geographical distribution is increasing, even within forested areas without cattle farms, which would require the existence of reservoir hosts other than cattle. Indeed, it was reported that B. divergens is also able to infect ungulates (roe deer, fallow deer, red deer, mouflon, and sheep), splenectomized rats, as well as non-splenectomized reindeer, sheep, and gerbil [see review in Ref. (347)]. Thus, this parasite has been shown to have a wider vertebrate host range than previously thought, leading to a potential risk not only in rural areas but also in peri-urban ones.

Babesia venatorum, implicated in human cases of babesiosis in Europe (343, 344), seems to phylogenetically lie in a sister group with B. divergens (343), and some serological cross-reactivity between B. divergens and B. venatorum has been reported (349). Roe deer were strongly suspected to be the wildlife reservoir of this parasite (350, 351) and its transmission by I. ricinus was validated both in vivo (351, 352) and in vitro (353). In addition, B. venatorum has been identified in I. ricinus in several European countries including Slovenia (354), Switzerland (355), the Netherlands (356), Poland (357), Italy (358), Belgium (359), Germany (37), and France (211, 351), with prevalence varying from 0.4 to 1.3%, demonstrating a wide geographical spread across the continent. Increasing reports of B. venatorum in ticks and wild ruminants make this parasite an excellent candidate for the emergence of a new zoonotic tick-borne disease, in particular in the current context of a growing number of wild hosts such as deer. As roe deer is often found even in suburban or peri-urban parks (if they are connected to more natural or semi-natural areas such as forests or rural areas), I. ricinus sampled in such places have already been reported as infected by B. venatorum (55, 323). This parasite has been detected in 1.3% of questing I. ricinus collected in France in a forest located in the South of Paris metropolitan area in the middle of an urban zone (211). Because of its location and the recreational activities available, this forest is visited by over 3 million people every year, emphasizing the public health risk. Similarly, the first detection of B. venatorum in Poland has been reported from ticks collected in an urban area (357), and a later study performed in recreational areas, corresponding to peri-urban forest near Warsaw city, showed also the presence of B. venatorum in questing I. ricinus (360).

Recent molecular phylogenetic investigations have convincingly established B. microti as forming a distinct and early diverging clade relative to other Babesia species (including the clade containing B. divergens and B. venatorum) as well as to Theileria species (361–363). B. microti is responsible for several hundred cases reported yearly in the USA in both spleen-intact and asplenic patient (132). This rodent parasite is known to be transmitted by I. ricinus, and now seems to be widely established in Europe, although only one human case has been reported to date (341). The substantial difference in the human pathogenicity of the North-American and European B. microti strains need further studies. It has been identified in I. ricinus in several European countries such as Switzerland (364), Poland (365), Hungary (366) Slovenia (367), Germany (368), the Netherlands (356, 369), Belgium (359), and France (340). Microtine rodents and probably shrews are the reservoirs of B. microti (Table 1). Infectious tick bites are most likely to occur in deciduous woodland and peri-domestic settings (37, 55, 323). Indeed, this parasite was recovered in questing ticks from a forest in Poland that was qualified as “one of the most popular tourist destinations in Poland,” highlighting the risk for humans during recreational activities (360).

New or neglected tick-borne pathogens: Still unknown bacterial, parasitic, and viral species to be discovered?

Due to advances in molecular biology, new species, strains, or genetic variants of microorganisms are being detected in ticks, resulting in an ever-increasing list of pathogens capable of infecting domesticated animals and humans. Some of them have been linked to human or animal diseases only many years after their first discovery in ticks or animal reservoirs (299). An emblematic example is that of B. henselae, the agent of Cat Scratch Disease, known to be transmitted from cat to human by cat scratch (or by fleas). For years, cases of B. henselae infection had been described in patients without history of contact with cat without any idea how these people could be infected. By screening pathogens in ticks, B. henselae DNA, and RNA were identified in I. ricinus (370–373). After many years of debate to know whether B. henselae was or was not a tick-borne pathogen, the direct link between tick bites, B. henselae, and disease in humans was finally demonstrated (374). Another striking example is the one of Borrelia miyamotoi. This Borrelia species has been isolated for the first time in Japan in 1995 from Ixodes ticks and has been considered as non-pathogenic endogenous tick bacteria until the first human cases of B. miyamotoi infection were reported in Russia in 2011 (375). Since then, human infections have been described in the USA and in 2013 in the Netherlands (376–379). In France, B. miyamotoi was found to circulate in I. ricinus as well as in the bank vole M. glareolus (380), and this French genotype was identical to the genotype isolated from a sick person in the Netherlands. These findings have important implications for public health, especially considering that B. miyamotoi-positive ticks and rodents were collected from different sites in close proximity to human dwellings. Up to now, no human cases of B. miyamotoi infections have been reported in most European countries, however, symptoms caused by B. miyamotoi could easily be confused with symptoms caused by other pathogens, which are better known by practitioners, suggesting that surveillance urgently needs to be improved.

A more recent example of neglected pathogens is a new phlebovirus that has been described in humans from northwestern Missouri, USA independently presented to a medical facility with fever, fatigue, diarrhea, thrombocytopenia, and leukopenia, and all had been bitten by ticks 5–7 days before the onset of illness. Electron microscopy revealed viruses consistent with members of the Bunyaviridae family. Next-generation sequencing and phylogenetic analysis identified the viruses as novel members of the phlebovirus genus (381). All these examples demonstrate that new or unexpected tick-borne pathogens are characterized, as soon as they are looked for, in patients bitten by ticks.

Conclusion

Tick-borne diseases in urban and peri-urban areas represent a rising hazard for public and animal health in Europe. The rapid global changes that planet Earth is facing, especially due to the human ecological footprint, are also affecting the ecology and epidemiology of infectious diseases, including tick-borne diseases. The I. ricinus tick being the principal vector of a plethora of viral, bacterial, and protozoan pathogenic microorganisms is showing adaptations to new habitats and ecological conditions. Persistent and potentially increasing populations of this tick species are present in green areas within European cities. Public parks, small forest patches, gardens, and cemeteries are of increasing interest as they represent places where humans, companion, and domestic animals can encounter ticks and be exposed to infected tick bites. The presence of large vertebrates, that serve as tick-maintenance hosts and find conditions to survive and reproduce in the peri-urban environment, reduces the extinction risk of tick populations. Furthermore, majority of tick-maintenance hosts are ecologically classified as generalist species and in many cases serve as reservoirs of a number of emerging zoonotic pathogens, including those transmitted by I. ricinus. The combination of urbanization, climate change, and alterations in land-use patterns along with socio-economic factors (outdoor sports and leisure-time activities, gardening, an increased density of pets, and companion animals near human settlements) act in creating favorable conditions for increasing the exposure of humans to ticks, thus favoring the transmission of tick-borne pathogens in urban and peri-urban areas.

Risk communication campaigns aimed at implementing preventive measures against infectious tick bites in urban and peri-urban habitats therefore deserve particular public health efforts. However, several knowledge gaps and lack of quantitative ecological, epidemiological, and socioecological data limit our ability to provide precise quantitative risk pre-assessment. Therefore, more eco-epidemiological research and surveillance specifically focused on the occurrence of ticks, their infection with pathogenic microorganisms as well as on the presence of tick-maintenance and reservoir vertebrate hosts in urbanized areas is urgently needed. Only a multidisciplinary “One-Health” approach integrating research outputs of specialists from different disciplines (veterinarians, zoologists, ecologists, molecular biologists, epidemiologists, physicians, sociologists, and public health experts, etc.), combined with appropriate outreach and dissemination campaigns, can bring success in making urban and peri-urban areas safer from infection by tick-borne pathogens.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.