Literature DB >> 25520453

Performance of the 47-kilodalton membrane protein versus DNA polymerase I genes for detection of Treponema pallidum by PCR in ulcers.

Angèle Gayet-Ageron1, Frédéric Laurent2, Jacques Schrenzel3, Béatrice Charton2, Gisela Jimenez-Getaz4, Manuela Tangomo5, Tristan Ferry6, Patrice Sednaoui7, Stephan Lautenschlager8, Laurence Toutous-Trellu9, Begoña Martinez de Tejada10, Matthias Cavassini11, Stéphane Emonet4, Thomas Perneger12, Hélène Salord2.   

Abstract

Treponema pallidum PCR (Tp-PCR) is a direct diagnostic method for primary and secondary syphilis, but there is no recommendation regarding the best choice of target gene. In this study, we sequentially tested 272 specimens from patients with sexually transmitted ulcers using Tp-PCR targeting the tpp47 and then polA genes. The two methods showed similar accuracies and an almost-perfect agreement.
Copyright © 2015, American Society for Microbiology. All Rights Reserved.

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Year:  2014        PMID： 25520453      PMCID： PMC4390634          DOI： 10.1128/JCM.03444-14

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  23 in total

1.  Molecular cloning of a gene (poIA) coding for an unusual DNA polymerase I from Treponema pallidum.

Authors:  Berta Rodes; Hsi Liu; Steven Johnson; Robert George; Bret Steiner
Journal:  J Med Microbiol       Date:  2000-07       Impact factor: 2.472

Review 2.  Syphilis: using modern approaches to understand an old disease.

Authors:  Emily L Ho; Sheila A Lukehart
Journal:  J Clin Invest       Date:  2011-12-01       Impact factor: 14.808

3.  2014 European guideline on the management of syphilis.

Authors:  M Janier; V Hegyi; N Dupin; M Unemo; G S Tiplica; M Potočnik; P French; R Patel
Journal:  J Eur Acad Dermatol Venereol       Date:  2014-10-27       Impact factor: 6.166

Review 4.  Sensitivity, specificity and likelihood ratios of PCR in the diagnosis of syphilis: a systematic review and meta-analysis.

Authors:  Angèle Gayet-Ageron; Stephan Lautenschlager; Béatrice Ninet; Thomas V Perneger; Christophe Combescure
Journal:  Sex Transm Infect       Date:  2012-09-28       Impact factor: 3.519

5.  Evaluation of a PCR test for detection of treponema pallidum in swabs and blood.

Authors:  P A Grange; L Gressier; P L Dion; D Farhi; N Benhaddou; P Gerhardt; J P Morini; J Deleuze; C Pantoja; A Bianchi; F Lassau; M F Avril; M Janier; N Dupin
Journal:  J Clin Microbiol       Date:  2012-01-04       Impact factor: 5.948

6.  Sexually transmitted diseases treatment guidelines, 2010.

Authors:  Kimberly A Workowski; Stuart Berman
Journal:  MMWR Recomm Rep       Date:  2010-12-17

7.  The epidemiological and clinical presentation of syphilis in a venereal disease centre in Paris, France. A cohort study of 284 consecutive cases over the period 2000-2007.

Authors:  David FARHI; Nada ZIZI; Philippe GRANGE; Nadjet BENHADDOU; Philippe GERHARDT; Marie-Françoise AVRIL; Nicolas DUPIN
Journal:  Eur J Dermatol       Date:  2009 Sep-Oct       Impact factor: 3.328

8.  Temporal trends in sexual behavior among men who have sex with men in the United States, 2002 to 2006-2010.

Authors:  Jami S Leichliter; Laura T Haderxhanaj; Harrell W Chesson; Sevgi O Aral
Journal:  J Acquir Immune Defic Syndr       Date:  2013-06-01       Impact factor: 3.731

9.  Assessment of a real-time PCR test to diagnose syphilis from diverse biological samples.

Authors:  A Gayet-Ageron; B Ninet; L Toutous-Trellu; S Lautenschlager; H Furrer; V Piguet; J Schrenzel; B Hirschel
Journal:  Sex Transm Infect       Date:  2009-01-20       Impact factor: 3.519

10.  Use of Treponema pallidum PCR in testing of ulcers for diagnosis of primary syphilis.

Authors:  Angèle Gayet-Ageron; Patrice Sednaoui; Stephan Lautenschlager; Tristan Ferry; Laurence Toutous-Trellu; Matthias Cavassini; Fatima Yassir; Begoña Martinez de Tejada; Stéphane Emonet; Christophe Combescure; Jacques Schrenzel; Thomas Perneger
Journal:  Emerg Infect Dis       Date:  2015-01       Impact factor: 6.883
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  4 in total

Review 1.  Comparison of Diagnostic Accuracy of PCR Targeting the 47-Kilodalton Protein Membrane Gene of Treponema pallidum and PCR Targeting the DNA Polymerase I Gene: Systematic Review and Meta-analysis.

Authors:  Angèle Gayet-Ageron; Christophe Combescure; Stephan Lautenschlager; Béatrice Ninet; Thomas V Perneger
Journal:  J Clin Microbiol       Date:  2015-08-26       Impact factor: 5.948

2.  Use of a Multiplex PCR Assay To Assess the Presence of Treponema pallidum in Mucocutaneous Ulcerations in Patients with Suspected Syphilis.

Authors:  P A Grange; A Jary; C Isnard; S Burrel; D Boutolleau; A Touati; C Bébéar; J Saule; P Martinet; J-L Robert; D Moulene; A Vermersch-Langlin; N Benhaddou; M Janier; N Dupin
Journal:  J Clin Microbiol       Date:  2021-01-21       Impact factor: 5.948

3.  Sensitive detection of Treponema pallidum DNA from the whole blood of patients with syphilis by the nested PCR assay.

Authors:  Cuini Wang; Yuanyuan Cheng; Biao Liu; Yuanyuan Wang; Weiming Gong; Yihong Qian; Zhifang Guan; Haikong Lu; Xin Gu; Mei Shi; Pingyu Zhou
Journal:  Emerg Microbes Infect       Date:  2018-05-09       Impact factor: 7.163

4.  Needle lost in the haystack: multiple reaction monitoring fails to detect Treponema pallidum candidate protein biomarkers in plasma and urine samples from individuals with syphilis.

Authors:  Geert A Van Raemdonck; Kara K Osbak; Xaveer Van Ostade; Chris R Kenyon
Journal:  F1000Res       Date:  2018-03-19
  4 in total

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