Literature DB >> 25519768

A sensitive detection of T4 polynucleotide kinase activity based on β-cyclodextrin polymer enhanced fluorescence combined with an exonuclease reaction.

Chunxia Song1, Xiaohai Yang, Kemin Wang, Qing Wang, Jianbo Liu, Jin Huang, Leiliang He, Pei Liu, Zhihe Qing, Wei Liu.   

Abstract

A strategy for T4 polynucleotide kinase activity detection was proposed based on a β-cyclodextrin polymer (polyβ-CD) and an exonuclease reaction. The fluorescence of pyrene enhanced by more than 10 times in the presence of polyβ-CD, and a simple detection of T4 PNK was achieved with a detection limit of 0.02 units per mL.

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Year:  2015        PMID: 25519768     DOI: 10.1039/c4cc08991j

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN: 1359-7345            Impact factor:   6.222


  4 in total

1.  A DNA functionalized porphyrinic metal-organic framework as a peroxidase mimicking catalyst for amperometric determination of the activity of T4 polynucleotide kinase.

Authors:  Weiling Song; Wenshuo Yin; Zhonghui Zhang; Peng He; Xiaoyan Yang; Xiaoru Zhang
Journal:  Mikrochim Acta       Date:  2019-02-02       Impact factor: 5.833

2.  Cobalt oxyhydroxide modified with poly-β-cyclodextrin and a cyanine dye as a nanoplatform for two-photon imaging of ascorbic acid in living cells and tissue.

Authors:  Huijuan Yan; Yufei Liu; Wu Ren; Jingfang Shangguan; Xue Yang
Journal:  Mikrochim Acta       Date:  2019-02-22       Impact factor: 5.833

Review 3.  Recent Advances in Nucleic Acid Targeting Probes and Supramolecular Constructs Based on Pyrene-Modified Oligonucleotides.

Authors:  Olga A Krasheninina; Darya S Novopashina; Evgeny K Apartsin; Alya G Venyaminova
Journal:  Molecules       Date:  2017-11-30       Impact factor: 4.411

4.  β-Cyclodextrin Polymer-Based Host-Guest Interaction and Fluorescence Enhancement of Pyrene for Sensitive Isocarbophos Detection.

Authors:  Shanshan Gao; Gege Yang; Xiaohui Zhang; Ying Lu; Ying Chen; Xiangwei Wu; Chunxia Song
Journal:  ACS Omega       Date:  2022-04-08
  4 in total

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