Literature DB >> 2551892

Tn5 mutagenesis of Anabaena sp. strain PCC 7120: isolation of a new mutant unable to grow without combined nitrogen.

D Borthakur1, R Haselkorn.   

Abstract

Methylation by Ava methylases in Escherichia coli increases the efficiency to transfer of Tn5 in pBR322bla:: Tn5 from E. coli to Anabaena sp. strain PCC 7120 by conjugation. Following conjugation, Tn5 but not pBR322 sequences were found at many different positions in the Anabaena chromosome. This procedure was used to mutagenize, tag, and clone a previously unrecognized gene required for nitrogen fixation in this Anabaena sp.

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Year:  1989        PMID： 2551892      PMCID： PMC210429          DOI： 10.1128/jb.171.10.5759-5761.1989

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  12 in total

1.  A restriction enzyme cleavage map of Tn5 and location of a region encoding neomycin resistance.

Authors:  R A Jorgensen; S J Rothstein; W S Reznikoff
Journal:  Mol Gen Genet       Date:  1979

2.  Conjugal transfer of DNA to cyanobacteria.

Authors:  J Elhai; C P Wolk
Journal:  Methods Enzymol       Date:  1988       Impact factor: 1.600

3.  Mutants of Anabaena cylindrica altered in heterocyst spacing.

Authors:  M Wilcox; G J Mitchison; R J Smith
Journal:  Arch Microbiol       Date:  1975-05-05       Impact factor: 2.552

4.  Isolation and physical mapping of nitrogen fixation genes from the cyanobacterium Anabaena 7120.

Authors:  D Rice; B J Mazur; R Haselkorn
Journal:  J Biol Chem       Date:  1982-11-10       Impact factor: 5.157

5.  New versatile plasmid vectors for expression of hybrid proteins coded by a cloned gene fused to lacZ gene sequences encoding an enzymatically active carboxy-terminal portion of beta-galactosidase.

Authors:  S K Shapira; J Chou; F V Richaud; M J Casadaban
Journal:  Gene       Date:  1983-11       Impact factor: 3.688

6.  Isolation and preliminary characterization of auxotrophs of a filamentous Cyanobacterium.

Authors:  T C Currier; J F Haury; C P Wolk
Journal:  J Bacteriol       Date:  1977-03       Impact factor: 3.490

7.  Classes of Anabaena variabilis mutants with oxygen-sensitive nitrogenase activity.

Authors:  J F Haury; C P Wolk
Journal:  J Bacteriol       Date:  1978-11       Impact factor: 3.490

8.  Construction of shuttle vectors capable of conjugative transfer from Escherichia coli to nitrogen-fixing filamentous cyanobacteria.

Authors:  C P Wolk; A Vonshak; P Kehoe; J Elhai
Journal:  Proc Natl Acad Sci U S A       Date:  1984-03       Impact factor: 11.205

9.  Construction of a broad host range cosmid cloning vector and its use in the genetic analysis of Rhizobium mutants.

Authors:  A M Friedman; S R Long; S E Brown; W J Buikema; F M Ausubel
Journal:  Gene       Date:  1982-06       Impact factor: 3.688

10.  Isolation and complementation of mutants of Anabaena sp. strain PCC 7120 unable to grow aerobically on dinitrogen.

Authors:  C P Wolk; Y Cai; L Cardemil; E Flores; B Hohn; M Murry; G Schmetterer; B Schrautemeier; R Wilson
Journal:  J Bacteriol       Date:  1988-03       Impact factor: 3.490
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  7 in total

1.  Synthesis of nitrogenase in mutants of the cyanobacterium Anabaena sp. strain PCC 7120 affected in heterocyst development or metabolism.

Authors:  A Ernst; T Black; Y Cai; J M Panoff; D N Tiwari; C P Wolk
Journal:  J Bacteriol       Date:  1992-10       Impact factor: 3.490

2.  A novel gene that bears a DnaJ motif influences cyanobacterial cell division.

Authors:  Olga A Koksharova; C Peter Wolk
Journal:  J Bacteriol       Date:  2002-10       Impact factor: 3.490

3.  Evidence that the hanA gene coding for HU protein is essential for heterocyst differentiation in, and cyanophage A-4(L) sensitivity of, Anabaena sp. strain PCC 7120.

Authors:  I Khudyakov; C P Wolk
Journal:  J Bacteriol       Date:  1996-06       Impact factor: 3.490

4.  The patB gene product, required for growth of the cyanobacterium Anabaena sp. strain PCC 7120 under nitrogen-limiting conditions, contains ferredoxin and helix-turn-helix domains.

Authors:  J Liang; L Scappino; R Haselkorn
Journal:  J Bacteriol       Date:  1993-03       Impact factor: 3.490

5.  The patA gene product, which contains a region similar to CheY of Escherichia coli, controls heterocyst pattern formation in the cyanobacterium Anabaena 7120.

Authors:  J Liang; L Scappino; R Haselkorn
Journal:  Proc Natl Acad Sci U S A       Date:  1992-06-15       Impact factor: 11.205

6.  Capsid Structure of Anabaena Cyanophage A-1(L).

Authors:  Ning Cui; Feng Yang; Jun-Tao Zhang; Hui Sun; Yu Chen; Rong-Cheng Yu; Zhi-Peng Chen; Yong-Liang Jiang; Shu-Jing Han; Xudong Xu; Qiong Li; Cong-Zhao Zhou
Journal:  J Virol       Date:  2021-09-22       Impact factor: 5.103

7.  Use of a transposon with luciferase as a reporter to identify environmentally responsive genes in a cyanobacterium.

Authors:  C P Wolk; Y Cai; J M Panoff
Journal:  Proc Natl Acad Sci U S A       Date:  1991-06-15       Impact factor: 11.205
  7 in total

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