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Literature DB >> 2551785

Nucleotide sequence and analysis of the Vibrio alginolyticus sucrase gene (scrB).

R R Scholle¹, S M Robb, F T Robb, D R Woods.

Abstract

The nucleotide sequence of a 2.119-kb DNA fragment containing the Vibrio alginolyticus sucrase gene (scrB) was determined. The complete sequence (484 aa residues) of the sucrase was deduced and homology was detected between the sucrase enzymes from V. alginolyticus and the Gram-positive bacteria Bacillus subtilis and Streptococcus mutans. In Escherichia coli cells the cloned V. alginolyticus sucrase is translocated to the periplasm. Transposon phoA mutagenesis experiments strongly suggested that V. alginolyticus sucrase in E. coli is not exported across the cytoplasmic membrane by means of a typical signal sequence.

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Year: 1989 PMID： 2551785 DOI： 10.1016/0378-1119(89)90249-7

Source DB: PubMed Journal: Gene ISSN： 0378-1119 Impact factor: 3.688

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Cited

15 in total

1. Molecular analysis of the scrA and scrB genes from Klebsiella pneumoniae and plasmid pUR400, which encode the sucrose transport protein Enzyme II Scr of the phosphotransferase system and a sucrose-6-phosphate invertase.

Authors: F Titgemeyer; K Jahreis; R Ebner; J W Lengeler
Journal: Mol Gen Genet Date: 1996-02-05

2. Purification and characterisation of an Aspergillus niger invertase and its DNA sequence.

Authors: L M Boddy; T Bergès; C Barreau; M H Vainstein; M J Dobson; D J Ballance; J F Peberdy
Journal: Curr Genet Date: 1993 Jul-Aug Impact factor: 3.886

3. Cloning, nucleotide sequence, and enzymatic characterization of an alpha-amylase from the ruminal bacterium Butyrivibrio fibrisolvens H17c.

Authors: E Rumbak; D E Rawlings; G G Lindsey; D R Woods
Journal: J Bacteriol Date: 1991-07 Impact factor: 3.490

4. Sequencing and expression of a cellodextrinase (ced1) gene from Butyrivibrio fibrisolvens H17c cloned in Escherichia coli.

Authors: E Berger; W A Jones; D T Jones; D R Woods
Journal: Mol Gen Genet Date: 1990-09

Nucleotide sequence and analysis of the Vibrio alginolyticus sucrase gene (scrB).

1. Molecular analysis of the scrA and scrB genes from Klebsiella pneumoniae and plasmid pUR400, which encode the sucrose transport protein Enzyme II Scr of the phosphotransferase system and a sucrose-6-phosphate invertase.

2. Purification and characterisation of an Aspergillus niger invertase and its DNA sequence.

3. Cloning, nucleotide sequence, and enzymatic characterization of an alpha-amylase from the ruminal bacterium Butyrivibrio fibrisolvens H17c.

4. Sequencing and expression of a cellodextrinase (ced1) gene from Butyrivibrio fibrisolvens H17c cloned in Escherichia coli.

5. Cloning and sequencing of the sacA gene: characterization of a sucrase from Zymomonas mobilis.

6. Nucleotide sequence of the celA gene encoding a cellodextrinase of Ruminococcus flavefaciens FD-1.

7. Molecular characterization of a fructanase produced by Bacteroides fragilis BF-1.

8. Characterization of the major promoter for the plasmid-encoded sucrose genes scrY, scrA, and scrB.

9. Sucrose fermentation by Fusobacterium mortiferum ATCC 25557: transport, catabolism, and products.

10. Cloning, sequencing, and disruption of a levanase gene of Bacillus polymyxa CF43.