Literature DB >> 2551570

Correlation of urine pH with the detection of cytomegalovirus by the shell vial technique.

L D Gray1, D M Ilstrup, T F Smith.   

Abstract

The influence of the pH of urine on the detection of cytomegalovirus (CMV) by the shell vial assay was evaluated. The pH values of 295 urine specimens ranged from 4.7 to 8.5 (mean 6.3) and were not significantly different in culture-positive versus culture-negative samples. None of the urine specimens appeared to be toxic for the cells used in the shell vial assay. We recommend inoculation of urine specimens into shell vials without adjustment of pH.

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Year:  1989        PMID： 2551570      PMCID： PMC7172990          DOI： 10.1016/0732-8893(89)90028-x

Source DB:  PubMed          Journal:  Diagn Microbiol Infect Dis        ISSN： 0732-8893            Impact factor:   2.803


  5 in total

1.  Immunological detection of cytomegalovirus early antigen on monolayers inoculated with urine specimens by centrifugation and cultured for 6 days as alternative to conventional virus isolation.

Authors:  H P Janssen; A M van Loon; M J Meddens; E C Eickmans-Josten; A J Hoitsma; T J de Witte; W G Quint
Journal:  J Clin Microbiol       Date:  1988-07       Impact factor: 5.948

2.  Rapid detection of cytomegalovirus in MRC-5 cells inoculated with urine specimens by using low-speed centrifugation and monoclonal antibody to an early antigen.

Authors:  C A Gleaves; T F Smith; E A Shuster; G R Pearson
Journal:  J Clin Microbiol       Date:  1984-06       Impact factor: 5.948

3.  Detection of cytomegalovirus in clinical specimens by virus isolation and by a monoclonal antibody against the early nuclear antigen.

Authors:  T Popow-Kraupp; C Kunz
Journal:  J Med Virol       Date:  1988-03       Impact factor: 2.327

4.  Rapid detection of cytomegalovirus by fluorescent monoclonal antibody staining and in situ DNA hybridization in a dram vial cell culture system.

Authors:  A F Sorbello; S L Elmendorf; J J McSharry; R A Venezia; R M Echols
Journal:  J Clin Microbiol       Date:  1988-06       Impact factor: 5.948

5.  Comparative study of inactivation of herpes simplex virus types 1 and 2 by commonly used antiseptic agents.

Authors:  W S Croughan; A M Behbehani
Journal:  J Clin Microbiol       Date:  1988-02       Impact factor: 5.948
  5 in total

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