Selcuk Erdem1, Gulcin Yegen2, Dilek Telci3, Ibrahim Yildiz4, Tzevat Tefik1, Halim Issever5, Isin Kilicaslan2, Oner Sanli6. 1. Department of Urology, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey. 2. Department of Pathology, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey. 3. Department of Genetics and Bioengineering, Yeditepe University, Istanbul, Turkey. 4. Department of Medical Oncology, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey. 5. Department of Biostatistics, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey. 6. Department of Urology, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey. sanlio@istanbul.edu.tr.
Abstract
PURPOSE: The aim of this study was to investigate the role of transglutaminase 2(TG2) in renal cell carcinoma (RCC) by comparing the immunohistochemistry staining of primary and metastatic tumor tissues. METHODS: A total of 33 metastatic RCC(mRCC) and 33 non-metastatic RCC (nmRCC) patients who were matched as closely as possible based on gender, age, nuclear grade and pathologic T stage were retrospectively investigated. TG2 immunohistochemistry staining was performed on paraffin-embedded primary tumor tissues from both patient groups and on metastatic tissues from mRCC patients. The tissues were scored from 0 to 7 according to the TG2 staining. Furthermore, the patients were stratified into two groups using median primary tumor staining score as the cutoff value: Group 1 (high risk, n = 41) and Group 2(low risk, n = 22). The clinical, histopathological and survival outcomes were compared between these risk groups using Chi-square test, t test, Mann-Whitney U test and Kaplan-Meier survival analyses. RESULTS: The median TG2 score for primary tumor was 5 for the entire study population. The median primary tumor TG2 score of the mRCC patients was significantly higher compared to the nmRCC patients (6 vs. 4, p < 0.001). The TG2 score between the primary and metastatic tissues of mRCC patients was not significantly different (6 vs. 7, p = 0.086). The percentage of metastatic patients was significantly higher in Group 1 compared to Group 2 (68.3 vs. 18.2 %, p < 0.001). Kaplan-Meier analyses showed that 5-year disease-free (34.9 vs. 92.9 %, p = 0.001) and cancer-specific (47.4 vs. 86.5 %, p = 0.04) survival rates were significantly lower in high-risk group. CONCLUSIONS: The increased expression of TG2 in primary tumor predicts metastasis in RCC patients and is also associated with a decrease in disease-free and cancer-specific survival outcomes.
PURPOSE: The aim of this study was to investigate the role of transglutaminase 2(TG2) in renal cell carcinoma (RCC) by comparing the immunohistochemistry staining of primary and metastatic tumor tissues. METHODS: A total of 33 metastatic RCC(mRCC) and 33 non-metastatic RCC (nmRCC) patients who were matched as closely as possible based on gender, age, nuclear grade and pathologic T stage were retrospectively investigated. TG2 immunohistochemistry staining was performed on paraffin-embedded primary tumor tissues from both patient groups and on metastatic tissues from mRCC patients. The tissues were scored from 0 to 7 according to the TG2 staining. Furthermore, the patients were stratified into two groups using median primary tumor staining score as the cutoff value: Group 1 (high risk, n = 41) and Group 2(low risk, n = 22). The clinical, histopathological and survival outcomes were compared between these risk groups using Chi-square test, t test, Mann-Whitney U test and Kaplan-Meier survival analyses. RESULTS: The median TG2 score for primary tumor was 5 for the entire study population. The median primary tumor TG2 score of the mRCC patients was significantly higher compared to the nmRCC patients (6 vs. 4, p < 0.001). The TG2 score between the primary and metastatic tissues of mRCC patients was not significantly different (6 vs. 7, p = 0.086). The percentage of metastatic patients was significantly higher in Group 1 compared to Group 2 (68.3 vs. 18.2 %, p < 0.001). Kaplan-Meier analyses showed that 5-year disease-free (34.9 vs. 92.9 %, p = 0.001) and cancer-specific (47.4 vs. 86.5 %, p = 0.04) survival rates were significantly lower in high-risk group. CONCLUSIONS: The increased expression of TG2 in primary tumor predicts metastasis in RCCpatients and is also associated with a decrease in disease-free and cancer-specific survival outcomes.
Authors: P Therasse; S G Arbuck; E A Eisenhauer; J Wanders; R S Kaplan; L Rubinstein; J Verweij; M Van Glabbeke; A T van Oosterom; M C Christian; S G Gwyther Journal: J Natl Cancer Inst Date: 2000-02-02 Impact factor: 13.506
Authors: Robert J Motzer; Eric Jonasch; Neeraj Agarwal; Clair Beard; Sam Bhayani; Graeme B Bolger; Sam S Chang; Toni K Choueiri; Ithaar H Derweesh; Shilpa Gupta; Steven L Hancock; Jenny J Kim; Timothy M Kuzel; Elaine T Lam; Clayton Lau; Ellis G Levine; Daniel W Lin; Kim A Margolin; M Dror Michaelson; Thomas Olencki; Roberto Pili; Elizabeth R Plimack; Edward N Rampersaud; Bruce G Redman; Charles J Ryan; Joel Sheinfeld; Kanishka Sircar; Brad Somer; Jue Wang; Richard B Wilder; Mary A Dwyer; Rashmi Kumar Journal: J Natl Compr Canc Netw Date: 2014-02 Impact factor: 11.908
Authors: B Min; H Park; S Lee; Y Li; J-M Choi; J Y Lee; J Kim; Y D Choi; Y-G Kwon; H-W Lee; S-C Bae; C-O Yun; K C Chung Journal: Oncogene Date: 2015-11-16 Impact factor: 9.867