Martin Thomas Freitag1, Gábor Márton2, Krisztián Pajer2, Jens Hartmann3, Nadja Walder3, Markus Rossmann3, Peter Parzer4, Heinz Redl3, Antal Nógrádi2,3, Bram Stieltjes1. 1. Quantitative Imaging-Based Disease Characterization, Department of Radiology, German Cancer Research Center, Heidelberg, Germany. 2. Laboratory of Neural Regeneration, Department of Anatomy, Histology, and Embryology, Faculty of Medicine, University of Szeged, Hungary. 3. Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Research Center of the AUVA, Vienna, Austria. 4. Section Disorders of Personality Development, Department of Child and Adolescent Psychiatry, Center for Psychosocial Medicine, University of Heidelberg, Germany.
Abstract
BACKGROUND AND PURPOSE: To evaluate the short-term outcome of erythropoietin (EPO) therapy in rats with spinal cord injury (SCI) using manganese-enhanced magnetic resonance imaging (MEMRI). METHODS: Rats were divided in an EPO and a control group. Laminectomy at Th11 was performed, followed by SCI. MnCl2 was applied into the cisterna magna and functional recovery was examined after injury using BBB-scoring. Then, rats were euthanized and the spinal cord was extracted for MEMRI. Finally, histological analysis was performed and correlated with MEMRI. RESULTS: EPO-treated animals showed significantly better functional recovery (P = .008, r = .62) and higher mean signal-to-noise ratio (SNR) in MEMRI compared to controls for slices 10-13 (P = .017, R(2) = .31) at the level of the lesion epicenter. Functional recovery correlated significantly with higher SNR values, determined using the mean SNR between slices 10 and 13 (P = .047, R(2) = .36). In this region, histology revealed a significantly decreased number of microglia cells and apoptosis in EPO-treated animals. CONCLUSION: MEMRI successfully depicts the therapeutic effect of EPO in early SCI that leads to a significant recovery in rats, a significantly reduced immune response and significantly reduced number of apoptotic cells at the height of the lesion epicenter.
BACKGROUND AND PURPOSE: To evaluate the short-term outcome of erythropoietin (EPO) therapy in rats with spinal cord injury (SCI) using manganese-enhanced magnetic resonance imaging (MEMRI). METHODS:Rats were divided in an EPO and a control group. Laminectomy at Th11 was performed, followed by SCI. MnCl2 was applied into the cisterna magna and functional recovery was examined after injury using BBB-scoring. Then, rats were euthanized and the spinal cord was extracted for MEMRI. Finally, histological analysis was performed and correlated with MEMRI. RESULTS:EPO-treated animals showed significantly better functional recovery (P = .008, r = .62) and higher mean signal-to-noise ratio (SNR) in MEMRI compared to controls for slices 10-13 (P = .017, R(2) = .31) at the level of the lesion epicenter. Functional recovery correlated significantly with higher SNR values, determined using the mean SNR between slices 10 and 13 (P = .047, R(2) = .36). In this region, histology revealed a significantly decreased number of microglia cells and apoptosis in EPO-treated animals. CONCLUSION: MEMRI successfully depicts the therapeutic effect of EPO in early SCI that leads to a significant recovery in rats, a significantly reduced immune response and significantly reduced number of apoptotic cells at the height of the lesion epicenter.
Authors: Bahram Biglari; Raban Arved Heller; Manuel Hörner; Andre Sperl; Tobias Bock; Bruno Reible; Patrick Haubruck; Paul Alfred Grützner; Arash Moghaddam Journal: J Spinal Cord Med Date: 2019-06-18 Impact factor: 1.985
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Authors: Alexander Younsi; Guoli Zheng; Lennart Riemann; Moritz Scherer; Hao Zhang; Mohamed Tail; Maryam Hatami; Thomas Skutella; Andreas Unterberg; Klaus Zweckberger Journal: Int J Mol Sci Date: 2021-12-03 Impact factor: 5.923