Alina Cristina Neguţ1, Oana Săndulescu2, Marcela Popa3, Anca Streinu-Cercel4, Zemphira Alavidze5, Ioana Berciu6, Coralia Bleotu7, Mircea Ioan Popa8, Mariana Carmen Chifiriuc9, Adrian Streinu-Cercel10. 1. MD, PhD Student, Carol Davila University of Medicine and Pharmacy; National Institute for Infectious Diseases "Prof. Dr. Matei Balş", Bucharest, Romania. 2. MD, Assistant Lecturer, Department of Infectious Diseases, Carol Davila University of Medicine and Pharmacy, National Institute for Infectious Diseases "Prof. Dr. Matei Balş", Bucharest, Romania. 3. PhD Student, Microbiology Department, Faculty of Biology, University of Bucharest; Research Institute of the University of Bucharest, Romania. 4. MD, PhD, Lecturer, Department of Infectious Diseases, Carol Davila University of Medicine and Pharmacy, National Institute for Infectious Diseases "Prof. Dr. Matei Balş", Bucharest, Romania. 5. PhD, Lead Scientist, "Eliava Biopreparation"; Phage Therapy Center, Tbilisi, Republic of Georgia. 6. MD, PhD Student, Department of Infectious Diseases, Carol Davila University of Medicine and Pharmacy; National Institute for Infectious Diseases "Prof. Dr. Matei Balş" 7. PhD, Microbiology Department, Faculty of Biology, University of Bucharest; Ştefan S. Nicolau Institute of Virology, Romanian Academy, Bucharest, Romania. 8. MD, PhD, Professor, Department of Microbiology II, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania. 9. PhD, Professor, Microbiology Department, Faculty of Biology, University of Bucharest; Research Institute of the University of Bucharest, Romania. 10. MD, PhD, Professor, Department of Infectious Diseases, Carol Davila University of Medicine and Pharmacy; National Institute for Infectious Diseases "Prof. Dr. Matei Balş", Bucharest, Romania.
Abstract
INTRODUCTION: Antimicrobial resistance is a growing threat for all clinical branches. This phenomenon poses important challenges in controlling infectious diseases. However, multidrug resistance is not the only issue, as bacteria that are otherwise susceptible to common antibiotics express other patterns for evading antibiotherapy, for example they can aggregate within a self-produced matrix to form biofilm. METHODS: We intend to perform a prospective laboratory study of the germs isolated from different samples collected from patients admitted with infectious pathology in reference hospitals in Romania. We will perform antibiotic resistance testing as well as phage testing, both on solid and liquid growth medium, for Staphylococcus spp., Enterococcus spp., and Pseudomonas spp. We intend to collect data for 150 patients with different infections with these identified pathogens. Phage susceptibility testing will be performed using 5 types of strain-specific bacteriophage mixtures: PYO, INTESTI, STAPHYLOCOCCAL (Eliava BioPreparations, Tbilisi, Georgia), PHAGYO, PHAGESTI (JSC "Biochimpharm", Tbilisi, Georgia). For phage-susceptible strains, we will evaluate biofilm formation in the presence of phages, as well as phage effect on already formed biofilm. EXPECTED RESULTS: Through this study, we intend to provide the first set of results on bacteriophage-susceptibility of bacteria isolated from patients with hard to treat infections, from reference hospitals in Romania. By evaluating a large number of bacterial strains we aim to predict and project biofilm kinetics, while adding binary phage dilutions at key timepoints during biofilm formation. ACKNOWLEDGMENTS: POSDRU/159/1.5/S/141531; Carol Davila University of Medicine and Pharmacy, Young Researchers Grant no. 28341/2013.
INTRODUCTION: Antimicrobial resistance is a growing threat for all clinical branches. This phenomenon poses important challenges in controlling infectious diseases. However, multidrug resistance is not the only issue, as bacteria that are otherwise susceptible to common antibiotics express other patterns for evading antibiotherapy, for example they can aggregate within a self-produced matrix to form biofilm. METHODS: We intend to perform a prospective laboratory study of the germs isolated from different samples collected from patients admitted with infectious pathology in reference hospitals in Romania. We will perform antibiotic resistance testing as well as phage testing, both on solid and liquid growth medium, for Staphylococcus spp., Enterococcus spp., and Pseudomonas spp. We intend to collect data for 150 patients with different infections with these identified pathogens. Phage susceptibility testing will be performed using 5 types of strain-specific bacteriophage mixtures: PYO, INTESTI, STAPHYLOCOCCAL (Eliava BioPreparations, Tbilisi, Georgia), PHAGYO, PHAGESTI (JSC "Biochimpharm", Tbilisi, Georgia). For phage-susceptible strains, we will evaluate biofilm formation in the presence of phages, as well as phage effect on already formed biofilm. EXPECTED RESULTS: Through this study, we intend to provide the first set of results on bacteriophage-susceptibility of bacteria isolated from patients with hard to treat infections, from reference hospitals in Romania. By evaluating a large number of bacterial strains we aim to predict and project biofilm kinetics, while adding binary phage dilutions at key timepoints during biofilm formation. ACKNOWLEDGMENTS: POSDRU/159/1.5/S/141531; Carol Davila University of Medicine and Pharmacy, Young Researchers Grant no. 28341/2013.
Authors: Leah C S Cepko; Eliotte E Garling; Madeline J Dinsdale; William P Scott; Loralee Bandy; Tim Nice; Joshua Faber-Hammond; Jay L Mellies Journal: J Med Microbiol Date: 2020-02-03 Impact factor: 2.472