Miho Hatanaka1, Yuko Higashi2, Tomoko Fukushige1, Naoko Baba1, Kazuhiro Kawai1, Teruto Hashiguchi3, Juan Su4, Weiqi Zeng4, Xiang Chen4, Takuro Kanekura1. 1. Department of Dermatology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan. 2. Department of Dermatology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan higashiy@m.kufm.kagoshima-u.ac.jp. 3. Department of Laboratory and Vascular Medicine, Cardiovascular and Respiratory Disorders, Advanced Therapeutics, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan. 4. Department of Dermatology, Xiangya Hospital, Central South University, Changsha, P.R. China.
Abstract
BACKGROUND: Cluster of differentiation 147 (CD147)/basigin on the malignant tumor cell surface is critical for tumor proliferation, invasiveness, metastasis, and angiogenesis. CD147 expressed on malignant melanoma cells can induce tumor cell invasion by stimulating the production of matrix metalloproteinases (MMPs) by surrounding fibroblasts. Membrane vesicles, microvesicles and exosomes have attracted attention, as vehicles of functional molecules and their association with CD147 has been reported. Cleaved CD147 fragments released from tumor cells were reported to interact with fibroblasts. We investigated the intercellular mechanisms by which CD147 stimulates fibroblasts to induce MMP2 activity. MATERIALS AND METHODS: CD147 was knocked-down using short hairpin RNA (shRNA). The stimulatory effect of CD147 in cell culture supernatants, microvesicles, and exosomes on the enzymatic activity of MMP2 was examined by gelatin zymography. RESULTS: Supernatants from A375 control cells induced increased enzymatic activity of fibroblasts; such activity was significantly lower in CD147 knock-down cells. CONCLUSION: Cleaved CD147 plays a pivotal role in stimulating fibroblasts to induce MMP2 activity. Copyright
BACKGROUND: Cluster of differentiation 147 (CD147)/basigin on the malignant tumor cell surface is critical for tumor proliferation, invasiveness, metastasis, and angiogenesis. CD147 expressed on malignant melanoma cells can induce tumor cell invasion by stimulating the production of matrix metalloproteinases (MMPs) by surrounding fibroblasts. Membrane vesicles, microvesicles and exosomes have attracted attention, as vehicles of functional molecules and their association with CD147 has been reported. Cleaved CD147 fragments released from tumor cells were reported to interact with fibroblasts. We investigated the intercellular mechanisms by which CD147 stimulates fibroblasts to induce MMP2 activity. MATERIALS AND METHODS:CD147 was knocked-down using short hairpin RNA (shRNA). The stimulatory effect of CD147 in cell culture supernatants, microvesicles, and exosomes on the enzymatic activity of MMP2 was examined by gelatin zymography. RESULTS: Supernatants from A375 control cells induced increased enzymatic activity of fibroblasts; such activity was significantly lower in CD147 knock-down cells. CONCLUSION: Cleaved CD147 plays a pivotal role in stimulating fibroblasts to induce MMP2 activity. Copyright
Authors: Agathe Quesnel; Amy Broughton; George S Karagiannis; Panagiota S Filippou Journal: Cancer Metastasis Rev Date: 2022-04-08 Impact factor: 9.264
Authors: Deepak K Kaushik; Heather Y F Yong; Jennifer N Hahn; Claudia Silva; Steven Casha; R John Hurlbert; Francois H Jacques; Robert Lisak; Omar Khan; Carolina Ionete; Catherine Larochelle; Alex Prat; Amit Bar-Or; V Wee Yong Journal: PLoS One Date: 2016-10-11 Impact factor: 3.240