| Literature DB >> 25501631 |
Wanwisa Dejnirattisai1, Wiyada Wongwiwat1, Sunpetchuda Supasa1,2,3, Xiaokang Zhang4,5, Xinghong Dai6, Alexander Rouvinski4,5, Amonrat Jumnainsong1,7, Carolyn Edwards1, Nguyen Than Ha Quyen8, Thaneeya Duangchinda9, Jonathan M Grimes10,11, Wen-Yang Tsai12, Chih-Yun Lai12, Wei-Kung Wang12, Prida Malasit2,9, Jeremy Farrar8, Cameron P Simmons8,13, Z Hong Zhou6, Felix A Rey4,5, Juthathip Mongkolsapaya1,2, Gavin R Screaton1.
Abstract
Dengue is a rapidly emerging, mosquito-borne viral infection, with an estimated 400 million infections occurring annually. To gain insight into dengue immunity, we characterized 145 human monoclonal antibodies (mAbs) and identified a previously unknown epitope, the envelope dimer epitope (EDE), that bridges two envelope protein subunits that make up the 90 repeating dimers on the mature virion. The mAbs to EDE were broadly reactive across the dengue serocomplex and fully neutralized virus produced in either insect cells or primary human cells, with 50% neutralization in the low picomolar range. Our results provide a path to a subunit vaccine against dengue virus and have implications for the design and monitoring of future vaccine trials in which the induction of antibody to the EDE should be prioritized.Entities:
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Year: 2014 PMID： 25501631 PMCID： PMC4445969 DOI： 10.1038/ni.3058
Source DB: PubMed Journal: Nat Immunol ISSN： 1529-2908 Impact factor: 25.606