INTRODUCTION: Rapid dissemination of plasmid-mediated quinolone resistance (PMQR) has been reported in clinical isolates. METHODOLOGY: A total of 149 clinical isolates of Enterobacteriaceae were collected in Beijing and screened for PMQR genes using polymerase chain reaction (PCR). Real-time quantitative PCR was used to study the expression of qnrS. RESULTS: The rates of qnr and aac(6')-Ib-cr genes were 7.4% and 8.1%, respectively. The higher basal expression of qnrS was observed in transconjugant strains, which had higher minimum inhibitory concentrations (MICs) of quinolones. Furthermore, qnrS expression levels increased in all three isolates when a quinolone was present. CONCLUSIONS: Our data suggest that the level of qnrS expression was associated with quinolone resistance.
INTRODUCTION: Rapid dissemination of plasmid-mediated quinolone resistance (PMQR) has been reported in clinical isolates. METHODOLOGY: A total of 149 clinical isolates of Enterobacteriaceae were collected in Beijing and screened for PMQR genes using polymerase chain reaction (PCR). Real-time quantitative PCR was used to study the expression of qnrS. RESULTS: The rates of qnr and aac(6')-Ib-cr genes were 7.4% and 8.1%, respectively. The higher basal expression of qnrS was observed in transconjugant strains, which had higher minimum inhibitory concentrations (MICs) of quinolones. Furthermore, qnrS expression levels increased in all three isolates when a quinolone was present. CONCLUSIONS: Our data suggest that the level of qnrS expression was associated with quinolone resistance.
Authors: Samira M Hamed; Walid F Elkhatib; Hadir A El-Mahallawy; Mai M Helmy; Mohamed S Ashour; Khaled M A Aboshanab Journal: Sci Rep Date: 2018-08-16 Impact factor: 4.379