Guanghui Gong1, Puxiang Chen2, Long Li1, Hong Tan1, Jun Zhou1, Yihong Zhou3, Xiaojing Yang1, Xiaoying Wu4. 1. Department of Pathology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China; Department of Pathology, School of Basic Medical Science, Central South University, Changsha, Hunan 410013, PR China. 2. Department of Gynecology and Obstetrics, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, PR China. 3. Department of Urology, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, PR China. 4. Department of Pathology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China; Department of Pathology, School of Basic Medical Science, Central South University, Changsha, Hunan 410013, PR China. Electronic address: xyw2007@csu.edu.cn.
Abstract
OBJECTIVE: To elucidate the different roles of lamin A and lamin C in the metastasis of epithelial ovarian cancer (EOC) by examining their expression in EOC tissues and cell lines and their correlations with clinicopathological features. METHODS: The expression of lamin A and lamin C was assessed in ovarian tissues from 61 EOC patients and 13 normal, 14 benign controls, respectively, using immunohistochemistry. Two pairs of EOC cell lines: HO-8910, HO-8910PM, SKOV-3 and SKOV-3ip were also examined to see the differential expression patterns of lamin A and lamin C. RESULTS: Lamin A expression was significantly lower in EOC tissues than that in normal and benign ovarian tissues (p<0.05), while lamin C expression was not. Lamin A expression level was closely associated with pathological grades, clinical stages, peritoneal metastasis and lymph node metastasis (all p<0.05). The progression-free survival rate of the EOC patients with low lamin A expression level was remarkably lower than that of the EOC patients with high expression level (p<0.05). Lamin A detected by PCR, Western Blot and immunocytochemistry also showed a significantly lower expression level in HO-8910PM than that in HO-8910 (p<0.05), but not for lamin C. There was no difference between SKOV-3 and SKOV-3ip cells. CONCLUSIONS: The results suggested that loss of lamin A but not lamin C expression in EOC was related to metastasis and poor prognosis. Lamin A may play a critical role in the metastasis of EOC.
OBJECTIVE: To elucidate the different roles of lamin A and lamin C in the metastasis of epithelial ovarian cancer (EOC) by examining their expression in EOC tissues and cell lines and their correlations with clinicopathological features. METHODS: The expression of lamin A and lamin C was assessed in ovarian tissues from 61 EOC patients and 13 normal, 14 benign controls, respectively, using immunohistochemistry. Two pairs of EOC cell lines: HO-8910, HO-8910PM, SKOV-3 and SKOV-3ip were also examined to see the differential expression patterns of lamin A and lamin C. RESULTS: Lamin A expression was significantly lower in EOC tissues than that in normal and benign ovarian tissues (p<0.05), while lamin C expression was not. Lamin A expression level was closely associated with pathological grades, clinical stages, peritoneal metastasis and lymph node metastasis (all p<0.05). The progression-free survival rate of the EOC patients with low lamin A expression level was remarkably lower than that of the EOC patients with high expression level (p<0.05). Lamin A detected by PCR, Western Blot and immunocytochemistry also showed a significantly lower expression level in HO-8910PM than that in HO-8910 (p<0.05), but not for lamin C. There was no difference between SKOV-3 and SKOV-3ip cells. CONCLUSIONS: The results suggested that loss of lamin A but not lamin C expression in EOC was related to metastasis and poor prognosis. Lamin A may play a critical role in the metastasis of EOC.
Authors: Emily S Bell; Pragya Shah; Noam Zuela-Sopilniak; Dongsung Kim; Alice-Anais Varlet; Julien L P Morival; Alexandra L McGregor; Philipp Isermann; Patricia M Davidson; Joshua J Elacqua; Jonathan N Lakins; Linda Vahdat; Valerie M Weaver; Marcus B Smolka; Paul N Span; Jan Lammerding Journal: Oncogene Date: 2022-07-27 Impact factor: 8.756
Authors: Elise Kaspi; Diane Frankel; Julien Guinde; Sophie Perrin; Sophie Laroumagne; Andrée Robaglia-Schlupp; Kevin Ostacolo; Karim Harhouri; Rachid Tazi-Mezalek; Joelle Micallef; Hervé Dutau; Pascale Tomasini; Annachiara De Sandre-Giovannoli; Nicolas Lévy; Pierre Cau; Philippe Astoul; Patrice Roll Journal: PLoS One Date: 2017-08-14 Impact factor: 3.240
Authors: Andrew D Stephens; Edward J Banigan; Stephen A Adam; Robert D Goldman; John F Marko Journal: Mol Biol Cell Date: 2017-01-05 Impact factor: 4.138