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Literature DB >> 2549953

A continuous fluorimetric assay for clostridial collagenase and Pz-peptidase activity.

A J Barrett¹, C G Knight, M A Brown, U Tisljar.

Abstract

The peptide derivative N alpha-(2,4-dinitrophenyl)-L-prolyl-L-leucyl-glycyl-L-prolyl-L-tryptophanyl-D- lysine (Dnp-Pro-Leu-Gly-Pro-Trp-D-Lys) has been found to be a convenient substrate for the assay of clostridial collagenase and Pz-peptidase. The substrate shows a 25-fold enhancement of fluorescence (gamma ex. 283 nm, lambda em. 350 nm) following hydrolysis of the Leu2-Gly3 peptide bond. The value of Km for clostridial collagenase was 17 microM. The substrate for the first time makes possible continuous fluorimetric assays for Pz-peptidase and clostridial collagenase.

Entities: Chemical Gene

Mesh：

Substances：

Year: 1989 PMID： 2549953 PMCID： PMC1138654 DOI： 10.1042/bj2600259

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

12 in total

1. Separation of two PZ-peptidases from bovine dental follicle.

Authors: M Hino; T Nagatsu
Journal: Biochim Biophys Acta Date: 1976-04-08

2. The free amino groups of haemoglobins.

Authors: R R Porter; F Sanger
Journal: Biochem J Date: 1948 Impact factor: 3.857

3. Substrate specificity of beta-collagenase from Clostridium histolyticum.

Authors: D R Steinbrink; M D Bond; H E Van Wart
Journal: J Biol Chem Date: 1985-03-10 Impact factor: 5.157

Review 4. Fluorescence energy transfer as a spectroscopic ruler.

Authors: L Stryer
Journal: Annu Rev Biochem Date: 1978 Impact factor: 23.643

5. Direct recording of the initially excited and the solvent relaxed fluorescence emission spectra of tryptophan by phase sensitive detection of fluorescence.

Authors: J R Lakowicz; A Balter
Journal: Photochem Photobiol Date: 1982-08 Impact factor: 3.421

5. A quenched fluorescent substrate for thimet peptidase containing a new fluorescent amino acid, DL-2-amino-3-(7-methoxy-4-coumaryl)propionic acid.

Authors: C G Knight
Journal: Biochem J Date: 1991-02-15 Impact factor: 3.857

6. Chicken liver Pz-peptidase, a thiol-dependent metallo-endopeptidase.

Authors: A J Barrett; M A Brown
Journal: Biochem J Date: 1990-11-01 Impact factor: 3.857

7. Human thimet oligopeptidase.

Authors: P M Dando; M A Brown; A J Barrett
Journal: Biochem J Date: 1993-09-01 Impact factor: 3.857

8. An internally quenched fluorogenic substrate of prohormone convertase 1 and furin leads to a potent prohormone convertase inhibitor.

Authors: F Jean; A Basak; J DiMaio; N G Seidah; C Lazure
Journal: Biochem J Date: 1995-05-01 Impact factor: 3.857

8 in total

A continuous fluorimetric assay for clostridial collagenase and Pz-peptidase activity.

1. Separation of two PZ-peptidases from bovine dental follicle.

2. The free amino groups of haemoglobins.

3. Substrate specificity of beta-collagenase from Clostridium histolyticum.

Review 4. Fluorescence energy transfer as a spectroscopic ruler.

5. Direct recording of the initially excited and the solvent relaxed fluorescence emission spectra of tryptophan by phase sensitive detection of fluorescence.

6. Intramolecularly quenched fluorogenic substrates for hydrolytic enzymes.

7. PZ-peptidase from chick embryos. Purification, properties, and action on collagen peptides.

8. The dinitrophenyl group as a selective label in high-performance liquid chromatography of peptides.

9. Identification and distribution of Pz-peptidases A and B in human semen.

10. Highly sensitive assay for PZ-peptidase activity by high-performance liquid chromatography.

1. Distinction between endo-oligopeptidase A (EC 3.4.22.19) and soluble metalloendopeptidase (EC 3.4.24.15)

2. Thimet oligopeptidase (EC 3.4.24.15): the same by any name?

3. Characterization of the bacterial metalloendopeptidase pitrilysin by use of a continuous fluorescence assay.

4. The activities of 'Pz-peptidase' and 'endopeptidase 24.15' are due to a single enzyme.

5. A quenched fluorescent substrate for thimet peptidase containing a new fluorescent amino acid, DL-2-amino-3-(7-methoxy-4-coumaryl)propionic acid.

6. Chicken liver Pz-peptidase, a thiol-dependent metallo-endopeptidase.

7. Human thimet oligopeptidase.

8. An internally quenched fluorogenic substrate of prohormone convertase 1 and furin leads to a potent prohormone convertase inhibitor.