Z Liu1, X Yu2, W Ren2, M Zhang1, H o Wang1, Y Sun1, L Jin3, F Wang1, M Shi4. 1. Research Center for Liver Transplantation, Beijing 302 Hospital, Beijing, China. 2. Peking University 302 Hospital, Beijing, China. 3. Research Center for Biological Therapy, Beijing 302 Hospital, Beijing, China. 4. Research Center for Liver Transplantation, Beijing 302 Hospital, Beijing, China. Electronic address: shiming302@sina.com.
Abstract
PURPOSE: To investigate CD152 and PD-1 expression on T lymphocytes and the function of CD152- and PD-1-positive CD8 T cells in human acute liver allograft rejection. MATERIALS AND METHODS: Sixty-three patients undergoing liver transplantation were enrolled in this study, including 26 cases with acute allograft rejection (Gr-AR) and 37 cases with stable allograft liver function (Gr-SF). The expression of CD152 and PD-1 on T lymphocytes and the expression of granzyme and perforin on CD152- and PD-1-positive CD8 T cells in peripheral blood were analyzed using flow cytometry. RESULTS: The peripheral CD4/CD8 ratio in Gr-AR was significantly lower than that in Gr-SF (P < .01). The expression of CD152 and PD-1 on CD8 and CD4 T cells was significantly lower in Gr-AR than in Gr-SF (all P < .01). The expression of granzyme B and perforin was significantly higher in Gr-AR than in Gr-SF (P < .01). CONCLUSIONS: Down-regulation of the expression of negative costimulatory molecules such as CD152 and PD-1 on CD8 T cells may be associated with human acute liver allograft rejection.
PURPOSE: To investigate CD152 and PD-1 expression on T lymphocytes and the function of CD152- and PD-1-positive CD8 T cells in human acute liver allograft rejection. MATERIALS AND METHODS: Sixty-three patients undergoing liver transplantation were enrolled in this study, including 26 cases with acute allograft rejection (Gr-AR) and 37 cases with stable allograft liver function (Gr-SF). The expression of CD152 and PD-1 on T lymphocytes and the expression of granzyme and perforin on CD152- and PD-1-positive CD8 T cells in peripheral blood were analyzed using flow cytometry. RESULTS: The peripheral CD4/CD8 ratio in Gr-AR was significantly lower than that in Gr-SF (P < .01). The expression of CD152 and PD-1 on CD8 and CD4 T cells was significantly lower in Gr-AR than in Gr-SF (all P < .01). The expression of granzyme B and perforin was significantly higher in Gr-AR than in Gr-SF (P < .01). CONCLUSIONS: Down-regulation of the expression of negative costimulatory molecules such as CD152 and PD-1 on CD8 T cells may be associated with human acute liver allograft rejection.
Authors: Nina C Sabins; Benjamin C Harman; Linda R Barone; Shixue Shen; Sandra Santulli-Marotto Journal: Front Immunol Date: 2016-06-16 Impact factor: 7.561