N Nomura1, K Saito2, M Ikeda1, S Yuasa1, M Pastore3, C Chabert3, E Kono4, A Sakai4, H Tanaka4, T Ikemoto4, T Takubo4. 1. HORIBA Medical, MiyanohigashiKisshoin, Minami-ku, Kyoto, Japan. 2. HORIBA Ltd, Tokyo Office: Kanda-Awaji cho, Kanda, Chiyoda-ku, Tokyo, Japan. 3. HORIBA ABX, Parc Euromédecine, Montpellier Cedex 4, France. 4. Department of Central Laboratory, Osaka Medical College Hospital, Daigaku-machi, Takatsuki, Japan.
Abstract
INTRODUCTION: We evaluated the basic performance of Microsemi CRP, an unique automated hematology analyzer which can simultaneously measure CBC including 3-part WBC differential (3-Diff) and CRP using whole blood treated with EDTA-2K anticoagulant. METHOD: We found that it produced generally the acceptable results for all parameters performed (repeatability, reproducibility, linearity, interference effect, carry over, and correlation) using control materials, fresh human whole bloods, and serum samples. RESULTS: CBC data examined using Microsemi CRP showed the good correlation with the previous model, Micros CRP200 (r ≧ 0.9), and also those obtained using the routine analyzer, ADVIA 2120i (r ≧ 0.989). Concerning the 3-Diff, both GRA (%) and LYM (%) showed the excellent correlation coefficient between Microsemi CRP and Micros CRP200 (r ≧ 0.992) as well as ADVIA 2120i (r ≧ 0.957). MON (%) showed good correlation between Microsemi CRP and Micros CRP200 (r = 0.959), but lower correlation between Microsemi CRP and ADVIA 2120 i (r = 0.471). CRP data showed the good correlation with HITACHI7600 (r ≧ 0.997) and Micros CRP200 (r ≧ 0.997). CONCLUSION: From these findings, we concluded that Microsemi CRP seemed the convenient laboratory analyzer in the setting of point of care testing (POCT) especially at NICU or primary care unit.
INTRODUCTION: We evaluated the basic performance of Microsemi CRP, an unique automated hematology analyzer which can simultaneously measure CBC including 3-part WBC differential (3-Diff) and CRP using whole blood treated with EDTA-2K anticoagulant. METHOD: We found that it produced generally the acceptable results for all parameters performed (repeatability, reproducibility, linearity, interference effect, carry over, and correlation) using control materials, fresh human whole bloods, and serum samples. RESULTS: CBC data examined using Microsemi CRP showed the good correlation with the previous model, Micros CRP200 (r ≧ 0.9), and also those obtained using the routine analyzer, ADVIA 2120i (r ≧ 0.989). Concerning the 3-Diff, both GRA (%) and LYM (%) showed the excellent correlation coefficient between Microsemi CRP and Micros CRP200 (r ≧ 0.992) as well as ADVIA 2120i (r ≧ 0.957). MON (%) showed good correlation between Microsemi CRP and Micros CRP200 (r = 0.959), but lower correlation between Microsemi CRP and ADVIA 2120 i (r = 0.471). CRP data showed the good correlation with HITACHI7600 (r ≧ 0.997) and Micros CRP200 (r ≧ 0.997). CONCLUSION: From these findings, we concluded that Microsemi CRP seemed the convenient laboratory analyzer in the setting of point of care testing (POCT) especially at NICU or primary care unit.
Keywords:
3-part WBC differential (3-Diff); Automated hematology analyzer Microsemi CRP; C-reactive protein (CRP); inflammatory disease; point of care testing (POCT)
Authors: Marilyn B Nourse; Kate Engel; Samartha G Anekal; Jocelyn A Bailey; Pradeep Bhatta; Devayani P Bhave; Shekar Chandrasekaran; Yutao Chen; Steven Chow; Ushati Das; Erez Galil; Xinwei Gong; Steven F Gessert; Kevin D Ha; Ran Hu; Laura Hyland; Arvind Jammalamadaka; Karthik Jayasurya; Timothy M Kemp; Andrew N Kim; Lucie S Lee; Yang Lily Liu; Alphonso Nguyen; Jared O'Leary; Chinmay H Pangarkar; Paul J Patel; Ken Quon; Pradeep L Ramachandran; Amy R Rappaport; Joy Roy; Jerald F Sapida; Nikolay V Sergeev; Chandan Shee; Renuka Shenoy; Sharada Sivaraman; Bernardo Sosa-Padilla; Lorraine Tran; Amanda Trent; Thomas C Waggoner; Dariusz Wodziak; Amy Yuan; Peter Zhao; Daniel L Young; Channing R Robertson; Elizabeth A Holmes Journal: Bioeng Transl Med Date: 2018-01-19