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Literature DB >> 2549365

Isolation and characterisation of transposon-induced mutants of Erwinia carotovora subsp. atroseptica exhibiting reduced virulence.

J C Hinton¹, J M Sidebotham, L J Hyman, M C Pérombelon, G P Salmond.

Abstract

The blackleg pathogen Erwinia carotovora subsp. atroseptica (Eca) causes an economically important disease of potatoes. We selected a genetically amenable Eca strain for the genetic analysis of virulence. Tn5 mutagenesis was used to generate nine mutants which exhibited reduced virulence (Rvi-) of strain SCRI1043. Following physiological characterisation, mutants were divided into three classes: (1) auxotrophs; (2) extracellular enzyme mutants; and (3) a growth rate mutant. The isolation of these Rvi- mutants has allowed us to consider some factors that affect Eca virulence.

Entities: Disease Species

Mesh：

Substances：

Year: 1989 PMID： 2549365 DOI： 10.1007/BF00330953

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

21 in total

1. Effects of a mutation that eliminates UDP glucose-pyrophosphorylase on the pathogenicity of Erwinia carotovora subsp. carotovora.

Authors: R K Jayaswal; R A Bressan; A K Handa
Journal: J Bacteriol Date: 1985-10 Impact factor: 3.490

2. Identification of a new virulence locus in Agrobacterium tumefaciens that affects polysaccharide composition and plant cell attachment.

Authors: M F Thomashow; J E Karlinsey; J R Marks; R E Hurlbert
Journal: J Bacteriol Date: 1987-07 Impact factor: 3.490

3. Chromosome transfer and R-prime formation by an RP4::mini-Mu derivative in Escherichia coli, Salmonella typhimurium, Klebsiella pneumoniae, and Proteus mirabilis.

Authors: F Van Gijsegem; A Toussaint
Journal: Plasmid Date: 1982-01 Impact factor: 3.466

Review 4. The use of transposon Tn5 mutagenesis in the rapid generation of correlated physical and genetic maps of DNA segments cloned into multicopy plasmids--a review.

Authors: F J de Bruijn; J R Lupski
Journal: Gene Date: 1984-02 Impact factor: 3.688

5. Characterization and virulence properties of Erwinia chrysanthemi lipopolysaccharide-defective, phi EC2-resistant mutants.

Authors: E Schoonejans; D Expert; A Toussaint
Journal: J Bacteriol Date: 1987-09 Impact factor: 3.490

6. Escherichia coli K-12 auxotrophs induced by insertion of the transposable element Tn5.

Authors: K J Shaw; C M Berg
Journal: Genetics Date: 1979-07 Impact factor: 4.562

7. Use of TnphoA to enrich for extracellular enzyme mutants of Erwinia carotovora subspecies carotovora.

Authors: J C Hinton; G P Salmond
Journal: Mol Microbiol Date: 1987-11 Impact factor: 3.501

8. Studies on transformation of Escherichia coli with plasmids.

Authors: D Hanahan
Journal: J Mol Biol Date: 1983-06-05 Impact factor: 5.469

9. pULB113, an RP4::mini-Mu plasmid, mediates chromosomal mobilization and R-prime formation in Erwinia amylovora, Erwinia chrysanthemi, and subspecies of Erwinia carotovora.

Authors: A K Chatterjee; L M Ross; J L McEvoy; K K Thurn
Journal: Appl Environ Microbiol Date: 1985-07 Impact factor: 4.792

10. Marker-exchange mutagenesis of a pectate lyase isozyme gene in Erwinia chrysanthemi.

Authors: D L Roeder; A Collmer
Journal: J Bacteriol Date: 1985-10 Impact factor: 3.490

12 in total

Review 1. Top 10 plant pathogenic bacteria in molecular plant pathology.

Authors: John Mansfield; Stephane Genin; Shimpei Magori; Vitaly Citovsky; Malinee Sriariyanum; Pamela Ronald; Max Dow; Valérie Verdier; Steven V Beer; Marcos A Machado; Ian Toth; George Salmond; Gary D Foster
Journal: Mol Plant Pathol Date: 2012-06-05 Impact factor: 5.663

2. Molecular cloning of ompRS, a regulatory locus controlling production of outer membrane proteins in Erwinia carotovora subsp. carotovora.

Authors: M B Karlsson; M Pirhonen; H T Saarilahti; E T Palva
Journal: Mol Gen Genet Date: 1991-05

3. Anaerobic Nitrate Respiration by Erwinia carotovora subsp. atroseptica during Potato Tuber Invasion.

Authors: E J Smid; A H Jansen; C J Tuijn
Journal: Appl Environ Microbiol Date: 1993-11 Impact factor: 4.792

Review 4. Quorum sensing in bacteria: the LuxR-LuxI family of cell density-responsive transcriptional regulators.

Authors: W C Fuqua; S C Winans; E P Greenberg
Journal: J Bacteriol Date: 1994-01 Impact factor: 3.490

5. Expression of pehA-bla gene fusions in Erwinia carotovora subsp. carotovora and isolation of regulatory mutants affecting polygalacturonase production.

Authors: H T Saarilahti; M Pirhonen; M B Karlsson; D Flego; E T Palva
Journal: Mol Gen Genet Date: 1992-07

6. Site-directed and transposon-mediated mutagenesis with pfd-plasmids by electroporation of Erwinia amylovora and Escherichia coli cells.

Authors: M Metzger; P Bellemann; T Schwartz; K Geider
Journal: Nucleic Acids Res Date: 1992-05-11 Impact factor: 16.971

7. lpxC and yafS are the most suitable internal controls to normalize real time RT-qPCR expression in the phytopathogenic bacteria Dickeya dadantii.

Authors: Florence Hommais; Ouafa Zghidi-Abouzid; Christine Oger-Desfeux; Emilie Pineau-Chapelle; Frederique Van Gijsegem; William Nasser; Sylvie Reverchon
Journal: PLoS One Date: 2011-05-26 Impact factor: 3.240

8. RelA-dependent (p)ppGpp production controls exoenzyme synthesis in Erwinia carotovora subsp. atroseptica.

Authors: Jinhong Wang; Noemie Gardiol; Tom Burr; George P C Salmond; Martin Welch
Journal: J Bacteriol Date: 2007-08-31 Impact factor: 3.490

9. Genome-Wide Analyses of the Temperature-Responsive Genetic Loci of the Pectinolytic Plant Pathogenic Pectobacterium atrosepticum.

Authors: Natalia Kaczynska; Ewa Lojkowska; Magdalena Narajczyk; Robert Czajkowski
Journal: Int J Mol Sci Date: 2021-05-03 Impact factor: 5.923

10. The lux autoinducer regulates the production of exoenzyme virulence determinants in Erwinia carotovora and Pseudomonas aeruginosa.

Authors: S Jones; B Yu; N J Bainton; M Birdsall; B W Bycroft; S R Chhabra; A J Cox; P Golby; P J Reeves; S Stephens
Journal: EMBO J Date: 1993-06 Impact factor: 11.598