| Literature DB >> 25491795 |
Nengsheng Ye1, Yali Xie2, Pengzhi Shi2, Ting Gao2, Jichao Ma2.
Abstract
In this study, a facile and novel strategy was developed to fabricate magnetite/graphene oxide/chitosan (Fe3O4/GO/CS) composite, and the composite was used as a magnetic adsorbent for the enrichment of protein, and followed by matrix-assisted laser desorption ionization mass spectrometry (MALDI-TOF MS) analysis. The phase composition, chemical structure and morphology of the composite were characterized by X-ray diffraction (XRD), Fourier transform infrared spectrometer (FTIR), transmission electron microscopy (TEM), scanning electronic microscope (SEM) and vibrating sample magnetometer (VSM). Protein cytochrome c was chosen as model target to evaluate the adsorptive property of Fe3O4/GO/CS. After enrichment procedure and magnetic separation, protein bounded with the material was analyzed by MALDI-TOF MS without desorption. The results indicated that Fe3O4/GO/CS composite exhibited a good adsorptive capacity for protein, and Fe3O4/GO/CS composite had a promising potential in magnetic separation research.Entities:
Keywords: Chitosan; Composite; Graphene oxide; Magnetite; Mass spectrometry; Protein enrichment
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Year: 2014 PMID： 25491795 DOI： 10.1016/j.msec.2014.08.064
Source DB: PubMed Journal: Mater Sci Eng C Mater Biol Appl ISSN： 0928-4931 Impact factor: 7.328