OBJECTIVE: To evaluate the effects of ginsenoside Rg-1 on the proliferation and osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) and to explore the possible application on the alveolar bone regeneration. METHODS: To determine the optimum concentration, the effects of ginsenoside Rg-1 ranging from 10 to 100 μmol/L were evaluated by 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide, alkaline phosphatase activity and calcium deposition. Expressions of runt-related transcription factor 2, collagen alpha-2(I) chain, osteopontin, osteocalcin protein were examined using real-time polymerase chain reaction. RESULTS: Compared with the control group, a certain concentration (10 μmol/L) of the Rg-1 solution significantly enhanced the proliferation and osteogenic differentiation of hPDLSCs (P<0.05). However, concentrations that exceeds 100 μmol/L led to cytotoxicity whereas concentrations below 10 nmol/L showed no significant effect as compared with the control. CONCLUSION: Ginsenoside Rg-1 can enhance the proliferation and osteogenic differentiation of hPDLSCs at an optimal concentration of 10 μmol/L.
OBJECTIVE: To evaluate the effects of ginsenosideRg-1 on the proliferation and osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) and to explore the possible application on the alveolar bone regeneration. METHODS: To determine the optimum concentration, the effects of ginsenosideRg-1 ranging from 10 to 100 μmol/L were evaluated by 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide, alkaline phosphatase activity and calcium deposition. Expressions of runt-related transcription factor 2, collagen alpha-2(I) chain, osteopontin, osteocalcin protein were examined using real-time polymerase chain reaction. RESULTS: Compared with the control group, a certain concentration (10 μmol/L) of the Rg-1 solution significantly enhanced the proliferation and osteogenic differentiation of hPDLSCs (P<0.05). However, concentrations that exceeds 100 μmol/L led to cytotoxicity whereas concentrations below 10 nmol/L showed no significant effect as compared with the control. CONCLUSION:GinsenosideRg-1 can enhance the proliferation and osteogenic differentiation of hPDLSCs at an optimal concentration of 10 μmol/L.
Entities:
Keywords:
Chinese medicine; ginsenoside Rg-1; human periodontal ligament stem cell; osteogenic differentiation; proliferation
Authors: Jiying Hou; Lu Wang; Chen Wang; Ruoxiang Ma; Ziling Wang; Hanxianzhi Xiao; Di Zeng; Li Ling; Yaping Wang Journal: Evid Based Complement Alternat Med Date: 2022-10-11 Impact factor: 2.650
Authors: Sang Ah Yi; Jieun Lee; Sun Kyu Park; Jeom Yong Kim; Jong Woo Park; Min Gyu Lee; Ki Hong Nam; Jee Hun Park; Hwamok Oh; Saetbyul Kim; Jihoon Han; Bo Kyung Kim; Dong-Gyu Jo; Jeung-Whan Han Journal: J Ginseng Res Date: 2018-08-08 Impact factor: 6.060