Literature DB >> 2549089

Comparison of in situ hybridization and monoclonal antibodies for early detection of cytomegalovirus in cell culture.

J T McClintock1, S R Thaker, M Mosher, D Jones, M Forman, P Charache, K Wright, J Keiser, F E Taub.   

Abstract

The abilities of each of four diagnostic tests--direct fluorescent monoclonal antibody (direct FA) staining, indirect fluorescent monoclonal antibody (indirect FA) staining, in situ hybridization with biotinylated DNA probes, and in situ hybridization with DNA probes directly linked to enzymatically active horseradish peroxidase-to detect cytomegalovirus soon after culture were compared. Only the indirect FA test and the in situ hybridization method with directly linked HRP-DNA probes provided consistent and reliable cytomegalovirus detection as early as 15 h postinfection.

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Year:  1989        PMID： 2549089      PMCID： PMC267614          DOI： 10.1128/jcm.27.7.1554-1559.1989

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  7 in total

1.  Comparative study of diagnostic procedures for congenital cytomegalovirus infection.

Authors:  S Stagno; R F Pass; D W Reynolds; M A Moore; A J Nahmias; C A Alford
Journal:  Pediatrics       Date:  1980-02       Impact factor: 7.124

2.  Rapid detection of cytomegalovirus in cell culture by indirect immunoperoxidase staining with monoclonal antibody to an early nuclear antigen.

Authors:  P D Swenson; M H Kaplan
Journal:  J Clin Microbiol       Date:  1985-05       Impact factor: 5.948

3.  Detection of cytomegalovirus from clinical specimens in centrifugation culture by in situ DNA hybridization and monoclonal antibody staining.

Authors:  C A Gleaves; D A Hursh; D H Rice; J D Meyers
Journal:  J Clin Microbiol       Date:  1989-01       Impact factor: 5.948

4.  Detection of cytomegalovirus in shell vial cultures by using a DNA probe and early nuclear antigen monoclonal antibody.

Authors:  A A Scott; K A Walker; L M Hennigar; C H Williams; J P Manos; T Gansler
Journal:  J Clin Microbiol       Date:  1988-09       Impact factor: 5.948

5.  Rapid detection of cytomegalovirus in MRC-5 cells inoculated with urine specimens by using low-speed centrifugation and monoclonal antibody to an early antigen.

Authors:  C A Gleaves; T F Smith; E A Shuster; G R Pearson
Journal:  J Clin Microbiol       Date:  1984-06       Impact factor: 5.948

6.  Rapid detection of cytomegalovirus by fluorescent monoclonal antibody staining and in situ DNA hybridization in a dram vial cell culture system.

Authors:  A F Sorbello; S L Elmendorf; J J McSharry; R A Venezia; R M Echols
Journal:  J Clin Microbiol       Date:  1988-06       Impact factor: 5.948

7.  Characterization of cytomegalovirus immediate-early genes. I. Nonpermissive rodent cells overproduce the IE94K protein form CMV (Colburn).

Authors:  K T Jeang; G Chin; G S Hayward
Journal:  Virology       Date:  1982-09       Impact factor: 3.616
  7 in total
  2 in total

1.  Optimization of in situ hybridization for detection of viral genomes in cultured cells on 96-microwell plates: a cytomegalovirus model.

Authors:  C Mougin; A Bassignot; A Coaquette; A Bourgeois; M Lab
Journal:  J Clin Microbiol       Date:  1991-08       Impact factor: 5.948

Review 2.  Physical and chemical methods for enhancing rapid detection of viruses and other agents.

Authors:  J H Hughes
Journal:  Clin Microbiol Rev       Date:  1993-04       Impact factor: 26.132
  2 in total

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