Simultaneous metering and dispensing of multiple reagents on a passively controlled microdevice solely by finger pressing.

In this work, we report a novel design of a passively controlled, finger-driven microfluidic circuit for the metering and delivery (MaD) of a liquid reagent. The proposed design modularized the fluidic circuit for a single reagent's MaD so that it can be multiplexed conveniently for the MaD of an arbitrary number of reagents solely by finger pressing. The microdevice has comparable accuracy with pipettes and we have demonstrated its applicability in the preparation of biochemical assays. The proposed design of the modularized, structurally "stackable" fluidic circuit provides a reference in designing future single-pressure-source-driven, passively controlled multi-liquid handling microfluidic platforms.