| Literature DB >> 2549015 |
R Skórko1, J Osipiuk, K O Stetter.
Abstract
Glycogen-bound polyphosphate kinase has been isolated from a crude extract of Sulfolobus acidocaldarius by isopycnic centrifugation in CsCl. Divalent cations (Mn2+ greater than Mg2+) stimulated the reaction. The enzyme does not require the presence of histones for its activity; it is inhibited strongly by phosphate and slightly by fluoride. The protein from the glycogen complex migrated in a sodium dodecyl sulfate-polyacrylamide gel as a 57-kilodalton protein band; after isoelectric focusing it separated into several spots in the pH range of 5.6 to 6.7.Entities:
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Year: 1989 PMID: 2549015 PMCID: PMC210331 DOI: 10.1128/jb.171.9.5162-5164.1989
Source DB: PubMed Journal: J Bacteriol ISSN: 0021-9193 Impact factor: 3.490