| Literature DB >> 25486551 |
Amal Ben Hassena1, Mohamed Barkallah1, Imen Fendri1, Noel Grosset2, Idriss Ben Neila3, Michel Gautier2, Radhouane Gdoura1.
Abstract
The objective of this study was to develop and evaluate a SYBR Green real time PCR method for the specific detection of Salmonella spp using a novel target, the siiA gene. Primer specificity testing was done on a panel of 76 Salmonella strains and 32 non-Salmonella strains. The primers directed against the siiA gene amplified all Salmonella strains tested, while non-Salmonella strains were not amplified. The melting temperatures of the 107 bp amplicons were consistently specific as they gave melting peaks around 75.5°C. The precision of the assay, based on intra and inter-run variations, was shown to be widely acceptable. In the second part of this study, 45 Salmonella strains were screened for the presence of 6 virulence-associated genes (sopB, cat2, safC, sefB and SC1248) located in several Salmonella Pathogenicity Islands (SPIs) and the spvC gene from the Salmonella virulence plasmid. The prevalence of these genes ranged from 51% to 100%. Variable virulence gene profiles were obtained even within the same serotype.Entities:
Keywords: Real time PCR; SPI; Salmonella detection; Virulence; spvC
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Year: 2014 PMID： 25486551 DOI： 10.1016/j.mimet.2014.11.018
Source DB: PubMed Journal: J Microbiol Methods ISSN： 0167-7012 Impact factor: 2.363