OBJECTIVES: To longitudinally assess the shedding of antimicrobial resistant Clostridium difficile strains by clinically healthy dogs raised at breeding facilities. METHODS: 18 puppies from three different litters (#1, 2 and 3) were sampled weekly from parturition to day 20-55 postpartum. Faecal samples from the mothers of litters #2 and 3 were also available for analysis. Bacterial isolates were ribotyped, tested for in vitro antimicrobial susceptibility and further characterised. RESULTS: C. difficile was recovered from all sampled animals of litters #1 and 2, and a third of puppies from litter #3, but marked differences in C. difficile recovery were detected in different age groups (0-100%). Recovered PCR ribotypes included 056 (22 isolates), 010 (6 isolates), 078 and 213 (2 isolates each), and 009 and 020 (1 isolate each). Different ribotypes were shed by four individual animals. Regardless of their origin and ribotype, all isolates demonstrated full resistance to levofloxacin. Additionally, all but one isolate (belonging to ribotype 078) were resistant to ertapenem, and all ribotype 010 isolates displayed high-level resistance to clindamycin, clarithromycin and erythromycin. A single ribotype 078 isolate showed metronidazole heteroresistance. CLINICAL SIGNIFICANCE: Healthy dogs can shed antimicrobial-resistant C. difficile strains.
OBJECTIVES: To longitudinally assess the shedding of antimicrobial resistant Clostridium difficile strains by clinically healthy dogs raised at breeding facilities. METHODS: 18 puppies from three different litters (#1, 2 and 3) were sampled weekly from parturition to day 20-55 postpartum. Faecal samples from the mothers of litters #2 and 3 were also available for analysis. Bacterial isolates were ribotyped, tested for in vitro antimicrobial susceptibility and further characterised. RESULTS:C. difficile was recovered from all sampled animals of litters #1 and 2, and a third of puppies from litter #3, but marked differences in C. difficile recovery were detected in different age groups (0-100%). Recovered PCR ribotypes included 056 (22 isolates), 010 (6 isolates), 078 and 213 (2 isolates each), and 009 and 020 (1 isolate each). Different ribotypes were shed by four individual animals. Regardless of their origin and ribotype, all isolates demonstrated full resistance to levofloxacin. Additionally, all but one isolate (belonging to ribotype 078) were resistant to ertapenem, and all ribotype 010 isolates displayed high-level resistance to clindamycin, clarithromycin and erythromycin. A single ribotype 078 isolate showed metronidazole heteroresistance. CLINICAL SIGNIFICANCE: Healthy dogs can shed antimicrobial-resistant C. difficile strains.
Authors: Sergio Álvarez-Pérez; José L Blanco; Teresa Peláez; Eva Martínez-Nevado; Marta E García Journal: Microb Ecol Date: 2016-04-26 Impact factor: 4.552
Authors: Sara Andrés-Lasheras; Inma Martín-Burriel; Raúl Carlos Mainar-Jaime; Mariano Morales; Ed Kuijper; José L Blanco; Manuel Chirino-Trejo; Rosa Bolea Journal: BMC Vet Res Date: 2018-03-09 Impact factor: 2.741
Authors: Alexander S F Berry; Brendan J Kelly; Denise Barnhart; Donna J Kelly; Daniel P Beiting; Robert N Baldassano; Laurel E Redding Journal: PLoS One Date: 2019-08-30 Impact factor: 3.240
Authors: Denise Rabold; Werner Espelage; Muna Abu Sin; Tim Eckmanns; Alexander Schneeberg; Heinrich Neubauer; Nadine Möbius; Katja Hille; Lothar H Wieler; Christian Seyboldt; Antina Lübke-Becker Journal: PLoS One Date: 2018-02-23 Impact factor: 3.240