| Literature DB >> 25482800 |
Makoto Ito1, Kouhei Ohnishi, Yasufumi Hikichi, Akinori Kiba.
Abstract
Ralstonia solanacearum is the causal agent of bacterial wilt disease. To better understand the molecular mechanisms involved in interaction between Nicotiana benthamiana and R. solanacearum, we focused on Hsp90, RAR1 and SGT1. Appearances of wilt symptom were significantly suppressed in Hsp90, RAR1 and SGT1-silenced plants compared with control plants. In RAR1-silenced plants, population of R. solanacearum increased in a similar manner to control plants. In contrast, multiplication of R. solanacearum was significantly suppressed in Hsp90 and SGT1-silenced plants. In addition, expression of PR genes were increased in Hsp90 and SGT1-silenced plants challenged with R. solanacearum. Therefore, RAR1 might be required for disease development or suppression of disease tolerance. These results also suggested that Hsp90 and/or SGT1 might play an important role in suppression of plant defenses leading to disease susceptibility and disease development.Entities:
Keywords: HR, hypersensitive response; Hsp, heat shock protein; Hsp90; Nicotiana benthamiana; PCR, polymerase chain reaction; RAR1, required for Mla12 resistance 1; RT-PCR, reverse transcription-polymerase chain reaction; SGT1 suppressor-of-G2-allele-of-skp1; TTSS, type III secretion system; VIGS, virus-induced gene silencing.; plant immunity; qRT-PCR, quantitative real time polymerase chain reaction; required for Mla12 resistance 1; suppressor-of-G2-allele-of-skp1; virus-induced gene silencing
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Year: 2015 PMID： 25482800 PMCID： PMC4622423 DOI： 10.4161/15592316.2014.970410
Source DB: PubMed Journal: Plant Signal Behav ISSN： 1559-2316