Kan-Tai Hsia1, Chung-Ji Liu1,2, Kwei Mar3, Li-Han Lin1, Chun-Shu Lin4, Ming-Fang Cheng1,5,6, Herng-Sheng Lee5, Shang-Yi Chiu1. 1. Institute of Oral Biology, School of Dentistry, National Yang-Ming University, Taipei, Taiwan. 2. Department of Oral and Maxillofacial Surgery, Taipei Mackay Memorial Hospital, Taipei, Taiwan. 3. Department of Dentistry, Zhongxiao Branch, Taipei City Hospital, Taipei, Taiwan. 4. Department of Radiation Oncology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan. 5. Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan. 6. Division of Histological and Clinical Pathology, Hualien Armed Forced General Hospital, Hualien, Taiwan.
Abstract
BACKGROUND: Apurinic/apyrimidinic endonuclease 1/redox factor-1 (APE1/Ref-1) is a multifunctional protein involved in DNA repair and redox signaling. The purpose of this study was to investigate the relationship between APE1/Ref-1 expression and clinicopathological features, survival, and treatment response in patients with oral squamous cell carcinoma (OSCC) and cell lines. METHODS: APE1/Ref-1 expression in OSCC was evaluated by immunohistochemistry, and its relationship to patient outcomes and treatment response was assessed statistically. The effects of stable short hairpin (sh)RNA-mediated knockdown of APE1/Ref-1 on cell survival, migration, and chemoradiation sensitivity were determined in OSCC cell lines. RESULTS: APE1/Ref-1 immunostaining was correlated with positive lymph node status, and higher APE1/Ref-1 expression was significantly associated with poor prognosis and reduced treatment response. Consistent with the clinical studies, APE1/Ref-1 expression in OSCC cell lines was implicated in the regulation of migration and cisplatin-induced apoptosis. CONCLUSION: Elevated APE1/Ref-1 expression may be used to predict poor survival and may confer chemoresistance in OSCC.
BACKGROUND: Apurinic/apyrimidinic endonuclease 1/redox factor-1 (APE1/Ref-1) is a multifunctional protein involved in DNA repair and redox signaling. The purpose of this study was to investigate the relationship between APE1/Ref-1 expression and clinicopathological features, survival, and treatment response in patients with oral squamous cell carcinoma (OSCC) and cell lines. METHODS:APE1/Ref-1 expression in OSCC was evaluated by immunohistochemistry, and its relationship to patient outcomes and treatment response was assessed statistically. The effects of stable short hairpin (sh)RNA-mediated knockdown of APE1/Ref-1 on cell survival, migration, and chemoradiation sensitivity were determined in OSCC cell lines. RESULTS:APE1/Ref-1 immunostaining was correlated with positive lymph node status, and higher APE1/Ref-1 expression was significantly associated with poor prognosis and reduced treatment response. Consistent with the clinical studies, APE1/Ref-1 expression in OSCC cell lines was implicated in the regulation of migration and cisplatin-induced apoptosis. CONCLUSION: Elevated APE1/Ref-1 expression may be used to predict poor survival and may confer chemoresistance in OSCC.
Authors: Leorik Pereira Silva; Thalita Santana; Bruno Tavares Sedassari; Suzana Machado de Sousa; Ana Paula Veras Sobral; Roseana de Almeida Freitas; Carlos Augusto Galvão Barboza; Lélia Batista de Souza Journal: Eur Arch Otorhinolaryngol Date: 2017-05-18 Impact factor: 2.503
Authors: Christina A Wicker; Vinita Takiar; Rangaswamy Suganya; Susanne M Arnold; Yolanda M Brill; Li Chen; Craig M Horbinski; Dana Napier; Joseph Valentino; Mahesh R Kudrimoti; Guoqiang Yu; Tadahide Izumi Journal: Oral Oncol Date: 2020-08-12 Impact factor: 5.337