Literature DB >> 25481282

Enzymatic and acidic degradation effect on intracellular polysaccharide of Flammulina velutipes SF-08.

Zhao Ma1, Chen Zhang1, Xia Gao2, Fangyuan Cui1, Jianjun Zhang1, Mengshi Jia3, Shouhua Jia4, Le Jia5.   

Abstract

The intracellular polysaccharide (IPS) from Flammulina velutipes SF-08 mycelia was isolated and degraded by enzyme and acid. IPS and its derivative were purified by DEAE-52 cellulose chromatography, and five fractions were obtained. The structural features and antioxidant activities in vitro of the isolated fractions were evaluated. On the basis of chemical composition and antioxidant ability analyses, rhamnose as the main monosaccharide might contribute to the strongest antioxidant capacity. The in vivo results showed that IPS significantly enhanced the activities of anti-aging enzymes, such as superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity, and reduced the content of lipid peroxidantion (LPO). These results suggested that IPS should be a potent natural polymer and can be developed to be novel functional food.
Copyright © 2014 Elsevier B.V. All rights reserved.

Entities:  

Keywords:  Enzymolysis and acidolysis; Flammulina velutipes SF-08; Intracellular polysaccharide

Mesh:

Substances:

Year:  2014        PMID: 25481282     DOI: 10.1016/j.ijbiomac.2014.11.028

Source DB:  PubMed          Journal:  Int J Biol Macromol        ISSN: 0141-8130            Impact factor:   6.953


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