| Literature DB >> 2547875 |
T T Hansel1, J D Pound, D Pilling, G D Kitas, M Salmon, T A Gentle, S S Lee, R A Thompson.
Abstract
A simple method for isolating highly purified eosinophils from human blood is described. Buffy coats from normal individuals (eosinophil counts less than 0.4 x 10(9)/litre) were centrifuged through a two layer Percoll density gradient, to produce a granulocyte fraction containing neutrophils and eosinophils. Neutrophils were extracted from this fraction using a monoclonal antibody (CLB FcR gran 1) against CD 16 (Fc gamma R III) in a direct or indirect selection procedure using immunomagnetic beads (Dynabeads). This negative immunoselection produced eosinophils of greater purity and with a superior capacity to mount a respiratory burst than eosinophils isolated by a method employing metrizamide.Entities:
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Year: 1989 PMID： 2547875 DOI： 10.1016/0022-1759(89)90339-6
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303