Deshant Agarwal1, Nilesh Pardhe2, Manas Bajpai1, Shailendra Gupta3, Nikunj Mathur4, S S Vanaki5, R S Puranik6, Manoj Mittal7. 1. Assistant Professor, Department of Oral and Maxillofacial Pathology, NIMS Dental College and Hospital , Jaipur, India . 2. Professor & Head, Department of Oral & Maxillofacial Pathology, NIMS Dental College & Hospital , Jaipur, Rajasthan, India . 3. Professor & Head, Department of Conservative Dentistry and Endodontics, RKDF Dental College , Bhopal, India . 4. Assistant Professor, Department of Oral Medicine & Radiology, Government Dental College , Jaipur, India . 5. Professor and Head, Department of Oral and Maxillofacial Pathology, PMNM Dental College and Hospital , Bagalkot, Karnataka, India . 6. Professor, Department of Oral and Maxillofacial Pathology, PMNM Dental College and Hospital , Bagalkot, Karnataka, India . 7. Professor & Head, Department of Periodontics, RKDF Dental College , Bhopal, India .
Abstract
OBJECTIVES: Lymphatic metastasis has always been regarded as a major prognostic indicator for disease progression and as a guide for therapeutic strategies to oral squamous cell carcinoma (OSCC). Differentiating lymphatic vessels from blood vessels is difficult, partly due to lack of specific method for identifying lymphatics. A new lymphatic vessel reactive antibody D2-40 has been introduced recently. Here we examined immunohistochemical localization of lymphatic vessels and blood vessels using D2-40 and CD-34 respectively in different histological grades of OSCC. Their expression in intra-tumoural and peri-tumoural region was also compared. MATERIALS AND METHODS: Forty two formalin-fixed paraffin-embedded tissue blocks of excised specimens of OSCC were immunohistochemically evaluated using D2-40 and CD-34 antibodies. Lymphatic vessel density (LVD) (D2-40 positivity) and micro vessel density (MVD) (CD34 positivity) in both intratumoural and peritumoural areas were assessed by hot spot method. RESULTS: Regardless of histopathological differentiation, LVD-- and MVD in peritumoural areas were found greater than intratumoural areas (p>0.05). Interestingly, other than lymphatic vessels, D2-40 positivity was also detected in tumour cells as well as in basal layer of epithelium adjacent to OSCC. Two patterns of distribution of CD34 positive vessel - circumscribing type and penetrating type were also observed in the cancer nest area. CONCLUSION: D2-40 can be used as a marker to differentiate lymphatic vessels from blood vessels. Lymphatic and blood vessel proliferation might be much more extensive in the peritumoural area. D2-40 expression in epithelium adjacent to tumour indicates its role in the process of differentiation. Further, its expression in potential malignant disorder may provide better insight in predicting prognosis and pathogenesis of these lesions.
OBJECTIVES: Lymphatic metastasis has always been regarded as a major prognostic indicator for disease progression and as a guide for therapeutic strategies to oral squamous cell carcinoma (OSCC). Differentiating lymphatic vessels from blood vessels is difficult, partly due to lack of specific method for identifying lymphatics. A new lymphatic vessel reactive antibody D2-40 has been introduced recently. Here we examined immunohistochemical localization of lymphatic vessels and blood vessels using D2-40 and CD-34 respectively in different histological grades of OSCC. Their expression in intra-tumoural and peri-tumoural region was also compared. MATERIALS AND METHODS: Forty two formalin-fixed paraffin-embedded tissue blocks of excised specimens of OSCC were immunohistochemically evaluated using D2-40 and CD-34 antibodies. Lymphatic vessel density (LVD) (D2-40 positivity) and micro vessel density (MVD) (CD34 positivity) in both intratumoural and peritumoural areas were assessed by hot spot method. RESULTS: Regardless of histopathological differentiation, LVD-- and MVD in peritumoural areas were found greater than intratumoural areas (p>0.05). Interestingly, other than lymphatic vessels, D2-40 positivity was also detected in tumour cells as well as in basal layer of epithelium adjacent to OSCC. Two patterns of distribution of CD34 positive vessel - circumscribing type and penetrating type were also observed in the cancer nest area. CONCLUSION:D2-40 can be used as a marker to differentiate lymphatic vessels from blood vessels. Lymphatic and blood vessel proliferation might be much more extensive in the peritumoural area. D2-40 expression in epithelium adjacent to tumour indicates its role in the process of differentiation. Further, its expression in potential malignant disorder may provide better insight in predicting prognosis and pathogenesis of these lesions.
Authors: Adhemar Longatto Filho; Tiago Gil Oliveira; Céline Pinheiro; Marcos Brasilino de Carvalho; Otávio Alberto Curioni; Ana Maria da Cunha Mercante; Fernando C Schmitt; Gilka Jf Gattás Journal: World J Surg Oncol Date: 2007-12-11 Impact factor: 2.754