Swati Kejriwal1, Rahul Bhandary2, Biju Thomas3, Suchetha Kumari4. 1. Postgraduate Student, Department of Periodontics, A.B.Shetty Memorial Institute of Dental Sciences , Mangalore, India . 2. Professor, Department of Periodontics, A. B. Shetty Memorial Institute of Dental Sciences , Mangalore, India . 3. Professor and Head of the Department, Department of Periodontics, A.B.Shetty Memorial Institute of Dental Sciences , Mangalore, India . 4. Professor, Department of Biochemistry, K.S.Hegde Medical Academy , Mangalore, India .
Abstract
BACKGROUND: Periodontal diseases are a group of inflammatory conditions resulting from interaction between a pathogenic bacterial biofilm and susceptible host's inflammatory response eventually leading to the destruction of periodontal structures and subsequent tooth loss. Hence, investigation of salivary proteins in individuals with periodontal diseases may be useful to enhance the knowledge of their roles in these diseases. MATERIALS AND METHODS: This case-control study was conducted at A.B. Shetty Memorial Institute of Dental Sciences, Mangalore. The study comprised of 90 patients of age between 25-60 years who were clinically examined and divided into three groups of 30 each: namely clinically healthy, gingivitis and chronic periodontitis. These were classified according to the values of gingival index score, clinical attachment loss and probing pocket depth. Unstimulated saliva was collected and salivary mucin, amylase and total protein levels were determined. STATISTICAL ANALYSIS: RESULTS obtained were tabulated and statistically analyzed using ANOVA test and Karl pearson's correlation test. RESULTS: The results of the study showed an increased concentration of salivary mucin, amylase and total protein in gingivitis patients and increased levels of amylase and total protein in saliva of chronic periodontitis patients compared to healthy individuals which were statistically significant. A decrease in mucin concentration was observed in the periodontitis group compared to gingivitis group. A positive correlation was present between salivary mucin, amylase and total protein levels in the three groups. CONCLUSION: Salivary mucin, amylase and total protein may serve as an important biochemical parameter of inflammation of the periodontium. Also, it can be hypothesized that various enzyme inhibitors might be useful as a part of host modulation therapy in the treatment of periodontal diseases.
BACKGROUND:Periodontal diseases are a group of inflammatory conditions resulting from interaction between a pathogenic bacterial biofilm and susceptible host's inflammatory response eventually leading to the destruction of periodontal structures and subsequent tooth loss. Hence, investigation of salivary proteins in individuals with periodontal diseases may be useful to enhance the knowledge of their roles in these diseases. MATERIALS AND METHODS: This case-control study was conducted at A.B. Shetty Memorial Institute of Dental Sciences, Mangalore. The study comprised of 90 patients of age between 25-60 years who were clinically examined and divided into three groups of 30 each: namely clinically healthy, gingivitis and chronic periodontitis. These were classified according to the values of gingival index score, clinical attachment loss and probing pocket depth. Unstimulated saliva was collected and salivary mucin, amylase and total protein levels were determined. STATISTICAL ANALYSIS: RESULTS obtained were tabulated and statistically analyzed using ANOVA test and Karl pearson's correlation test. RESULTS: The results of the study showed an increased concentration of salivary mucin, amylase and total protein in gingivitispatients and increased levels of amylase and total protein in saliva of chronic periodontitispatients compared to healthy individuals which were statistically significant. A decrease in mucin concentration was observed in the periodontitis group compared to gingivitis group. A positive correlation was present between salivary mucin, amylase and total protein levels in the three groups. CONCLUSION: Salivary mucin, amylase and total protein may serve as an important biochemical parameter of inflammation of the periodontium. Also, it can be hypothesized that various enzyme inhibitors might be useful as a part of host modulation therapy in the treatment of periodontal diseases.
Authors: J Groenink; E Walgreen-Weterings; K Nazmi; J G Bolscher; E C Veerman; A J van Winkelhoff; A V Nieuw Amerongen Journal: J Clin Periodontol Date: 1999-05 Impact factor: 8.728
Authors: Vlad Dinu; Chujiao Liu; Joseph Ali; Charfedinne Ayed; Pavel Gershkovich; Gary G Adams; Stephen E Harding; Ian D Fisk Journal: Sci Rep Date: 2018-09-06 Impact factor: 4.379