Pemetrexed for previously treated patients with metastatic gastric cancer: a prospective phase II study.

BACKGROUND: Gastric cancer is one of the leading causes of cancer-related death worldwide. There were debates on the value of the second-line and beyond chemotherapy by the time we designed this trial. So we designed this phase II trial to assess the efficacy and safety of pemetrexed in patients with pretreated metastatic gastric cancer.
METHODS: Thirty-four patients with pretreated metastatic gastric cancer were enroled in the study. Patients received pemetrexed 500 mg m(-2) every 21 days until the presence of progressive disease (PD) or unacceptable toxicity.
RESULTS: A total of 34 patients were enroled in the study; 34 were eligible for toxicity and 30 for response. The response rate was 13.3%, 13.3% patients achieved a partial response, 50.0% achieved stable disease and 36.7% had a PD as the best response. The median overall survival time and median progression-free survival time was 6.4 months (95% confidence interval (CI) 5.8-9.5 months) and 2.2 months (95% CI 2.0-5.5 months), respectively. Most haematologic and non-haematologic toxicity were grade 1/2. Grade 3/4 toxicity included fatigue, neutropenia, thrombocytopenia, weight loss, anorexia and transaminase elevation.
CONCLUSIONS: The monochemotherapy of pemetrexed is active and well tolerated when used in previously treated patients with metastatic gastric cancer.

Gastric cancer is the second leading cause of cancer-related death worldwide, with hundreds of thousands of newly diagnosed cases each year (Jemal ). Although surgical resection is the only curative treatment, many patients with metastatic disease have lost the opportunity to receive surgery due to late diagnosis (Wang ). For such patients, palliative chemotherapy is the mainstay treatment to prolong their survival. Systemic chemotherapy based on 5-fluoropyrimide (5-FU)/platinum plus human epidermal growth factor receptor-2 antibody or not has proved to be effective first-line therapy (Demolin ; Bang ). But the response rate of first-line therapy is limited and the duration of responses was only a few months, which means that many patients who retain a good performance after previous chemotherapy remain good candidates for further treatment.

When designing this trial, there were debates on the value of the second-line and beyond chemotherapy. Some physicians considered that administration of salvage chemotherapy will only add to patients suffering without prolonging their life. Some argued that improvements in the overall survival (OS) as well as the quality of life could be found in the patients who received an active and tolerable chemotherapy after carefully selecting (Park ; Ji ; Wesolowski ). Although survival benefits were seen in some phase II trials with different chemotherapy regimens, there was no standard regimen for pretreated metastatic gastric cancer.

Pemetrexed is a pyrrolopyrimidine antifolate that has established its promising efficacy in the treatment of patients with advanced mesotholioma and non-small cell lung cancer. It is referred to as a multitargeted antifolate because it inhibits at least three folate-dependent enzymes, including thymidylate synthase, dihydrofolate reductase and glycinamide ribonucleotide formyl transferase (Shih ). The clinical activity has also been seen in some clinical trials for treatment of bladder, breast, colon, pancreas and gastric cancer (Kut ). Previous phase II trials have shown that, when used either as a single agent or in combination as first-line chemotherapy, pemetrexed is an acceptable safety profile in metastatic gastric cancer, with beneficial activity (RR 21–36% median OS 6.6–11.8 months; median progression-free survival (PFS) 2.8–6.2 months; Bajetta ; Kim ; Celio ; Chen ). As we know, there are few clinical trials that reported the activity and tolerability of pemetrexed as salvage monochemotherapy in metastatic gastric cancer.

The aim of this phase II study is to evaluate the efficacy and safety of single-agent pemetrexed in previously treated patients with metastatic gastric cancer.

Patients and methods

Patients

Eligible criteria included histologically confirmed inoperable or metastatic adenocarcinoma of the stomach or gastro–oesophageal junction, at least one prior chemotherapy regimen, measurable disease by Response Evaluation Criteria in Solid Tumors (RECIST), age between 18 and 80 years, ECOG performance status of 0–2 and life expectancy ⩾12 weeks. Exclusion criteria included other primary malignancy, symptomatic central nervous system metastasis, pregnancy or lactation, cardiovascular events in the previous 6 months or congestive heart failure, ongoing infection, inability or unwillingness to take nonsteroidal anti-inflammatory drugs or folic acid or vitamin B12 supplementation.

Patients and disease evaluations

Baseline examination, performed within 3 weeks of commencing treatment, included a complete history and physical examination, complete blood count, blood chemistries, urinalysis, electrocardiogram, tumour markers and a computed tomography (CT) scan of chest, abdomen and pelvis. One study's evaluations (every 21 days) included a limited history and physical examination, complete blood count and blood chemistries, performance status evaluation and toxicity rating. Toxicity evaluations were based on the Common Terminology Criteria for Adverse Event (CTCAE) Version 3.0. Disease assessment by CT scan was performed every two cycles of therapy to ensure that patients with other treatment options were not rapidly progressing. Response to treatment was assessed by means of CT scan using RECIST guidelines (version 1.1) every two cycles of treatment.

Study medications

Patients received pemetrexed 500 mg m−2, once every 21 days, until the presence of progressive disease (PD) or unacceptable toxicity. All patients took dexamethasone 3.75 mg twice daily, starting from the day before and continuing to the day after the drug administration. Patients were instructed to take oral folic acid 400 mg daily beginning 5 days before the first therapy and until the final therapy. Treatments would continue until unacceptable toxicity or disease progression occurred. In addition, dose adjustment was based on the toxicity of the preceding administration of pemetrexed. If a patient developed grade 4 neutropenia, grade 3 febrile neutropenia or grade 3 thrombopenia, drug dosage was reduced by 25% in the subsequent treatment cycles. If a patient developed grade 4 febrile neutropenia or grade 4 thrombopenia, drug dosage was reduced by 50% in the subsequent treatment cycles. If a patient develops a grade 3–4 mucositis, pharyngitis, oesophagitis or diarrhoea during treatment, drug dosage was reduced by 25% in the subsequent treatment cycles only after the toxicity had been resolved to less than grade 2. If drug administration was delayed for more than 4 weeks, the patient was excluded from the study. Treatment would not be interrupted when a patient had a toxicity that had no clinical influence.

Study design and statistical analysis

This is a phase II, nonrandomized, open-label trial. The primary end point of this study was ORR. A Simon's two-stage design was utilised to calculate sample size with p0=0.05 (null hypothesis) and p1=0.25 (alterative hypothesis), with a significance level of 0.05 and a power of 90% (Simon, 1989). A sample size of 9 is needed for the first stage, and if at least 1 of these patients achieved objective response, further 21 patients were to be recruited in the second stage. The rate of loss to follow-up was expected to be <15%. Therefore, the sample size was increased to 10 in the first stage and 24 in the second stage for a total sample size of 34 patients. ORR was evaluated in patients who received at least one dose of pemetrexed and underwent at least one post-baseline assessment. Patients who received at least one cycle of treatment were included in the safety analysis. The regimen was considered to be active if four or more patients had an objective response. The secondary end points of this study included OS, PFS, DCR and the toxicity profile. OS was measured from the date of consent to the time of death, and PFS from the date of consent to the first observation of PD. All patients were to be followed until death or 30 April 2014. OS and PFS were analysed using the Kaplan–Meier method. Exact 95% confidence interval (CI) was estimated for the variables. The analyses were performed using SPSS (Statistical Package for the Social Sciences) 16.0 software for the Windows operating system (SPSS Inc, Chicago, IL, USA).

The study was approved by the Ethical Review Board of Sun Yat-sen University Cancer Center, and followed the ethical principles of Good Clinical Practice in accordance with the Declaration of Helsinki. All patients signed a written informed consent document before enrolment. The clinical trial was registered with clinicaltrials.gov (identifier NCT01953419).

Results

Patient characteristics

From October 2010 to December 2013, a total of 34 patients were enroled in the study. Baseline patient characteristics are summarised in Table 1. Majority of the patients are male (58.8%), with a median age of 49 years (range 27–80). In total, 7 (20.6%) of the patients had an ECOG performance status of 0, 20 (58.8%) had an ECOG performance status of 1 and the rest 7 (20.6%) had an ECOG performance status of 2. Of the 34 patients, 25 (73.5%) received pemetrexed as second-line treatment and 9 (26.5%) as third- or fourth-line treatment. Six patients had prior curative gastric resection and 11 received prior palliative surgery. Most frequent metastatic sites are lymph node (55.9%), peritoneum (47.1%) and liver (38.2%).

Table 1

Patient characteristics (n=34)

Patient characteristics	n (%)
Age, years
Median	49
Range	27–80
Gender
Male	20 (58.8)
Female	14 (41.2)
ECOG
0	7 (20.6)
1	20 (58.8)
2	7 (20.6)
Number of prior regimens
1	25 (73.5)
⩾2	9 (26.5)
Prior surgery
None	17 (50.0)
Curative	6 (17.6)
Palliative	11 (32.4)
Number of organ involved
1	8 (23.5)
2	16 (47.1)
⩾3	10 (29.4)
Disease sites
Lymph node	19 (55.9)
Peritoneum	16 (47.1)
Liver	13 (38.2)
Lung	6 (17.6)
Bone	4 (11.7)
Others	3 (8.8)

Abbreviation: ECOG=Eastern Cooperative Oncology Group.

Treatment summary

Among the 34 patients enroled, 30 patients were assessable. Two patients who had an ECOG performance status of 2 had deterioration in their physical condition not considered to be related to the chemotherapy and stopped chemotherapy without a CT reassessment. One patient refused to take a CT assessment after treatment. One patient with multiple peritoneal metastases turned to receive best supportive care due to severe hypoproteinaemia. This left 30 patients in the primary analysis and 34 patients in the toxicity analysis. The patients enroled in this study received a median of three cycles of pemetrexed, ranged from 1 to 6. Of the 30 patients qualified for efficacy analysis, only 1 patient required a dose reduction because of intolerable toxicity.

Efficacy

The details of treatment efficacy are shown in Table 2. Four of 30 assessable patients (13.3%) achieved a partial response (PR), 15 patients (50%) achieved stable disease (SD) and 11 patients (36.7%) had a PD as the best response (ORR 13.3% DCR 63.3%). Among 22 patients who received pemetrexed as a second-line chemotherapy, 4 patients (18.2%) achieved PR, 10 patients (45.4%) achieved SD and the rest had a PD (ORR 18.2% DCR 63.6%). Among eight patients who previously received two or more lines of chemotherapy, five patients (62.5%) achieved SD and the rest had a PD (ORR 0.0% DCR 62.5%). Of the 30 assessable patients who received pemetrexed, 2 were alive at the last follow-up; the median OS was 6.4 months (95% CI 5.8–9.5 months) and 6.4 months (95% CI 5.2–10.0 months) for the second-line group and 6.0 months (95% CI 4.5–11.2 months) for the third-line and beyond group; the median PFS was 2.2 months (95% CI 2.0–5.5 months) and 2.3 months (95% CI 1.9–6.5 months) for the second-line group and 2.2 months (95% CI 1.3–4.1 months) for the third-line and beyond group.

Table 2

Treatment response of all assessable patients and by previous treatment group

	All patients (n=30)	Second-line group (n=22)	Third-line and beyond group (n=8)
Response
PR	4 (13.3%)	4 (18.2%)	0 (0.0%)
SD	15 (50.0%)	10 (45.4%)	5 (62.5%)
PD	11 (36.7%)	8 (36.4%)	3 (37.5%)
ORR (95% CI)	13.3%	18.2%	0.0%
DCR (95% CI)	63.3%	63.6%	62.5%

Abbreviations: CI=confidence interval; PD=progressive disease; PR=partial response; SD=stable disease.

Safety

All 34 patients who were enroled in the study were evaluated for toxicity. The toxicities were summarised in Table 3. There was no treatment-related mortality. Totally, 27 (79.4%) patients had at least one adverse event, most commonly anaemia, anorexia and nausea. Most haematologic and non-haematologic toxicities were grade 1 or 2. Grade 3 or 4 toxicity included fatigue, neutropenia, thrombocytopenia, weight loss, anorexia and transaminase elevation. Three patients discontinued the treatment because of worse performance status or severe hypoproteinaemia after the first cycle. No one delayed the treatment, but one patient required a dose reduction due to grade 4 thrombopenia.

Table 3

Toxicity of the patients enroled in the study (worst grade per patients; n=34)

Adverse Event	All grades(no.)	%	Grade 3 or 4(no.)	%
Any AEs	27	79.4
Haematologic AEs
Neutropenia	8	23.5	1	2.9
Leukopenia	9	26.5	0	0
Anaemia	19	55.9	1	2.9
Thrombocytopenia	4	11.8	1	2.9
Non-haematologic AEs
Nausea	10	29.4	0	0
Anorexia	13	38.2	1	2.9
Vomiting	4	11.8	0	0
Diarrhoea	1	2.9	0	0
Constipation	2	5.8	0	0
Fatigue	9	26.5	2	5.8
Weight loss	7	20.6	1	2.9
Bilirubin	5	14.7	0	0
Transaminases	11	32.4	1	2.9
Neutropathy	1	2.9	0	0
Alopecia	1	2.9	0	0
Rash	2	5.8	0	0

Discussion

The result of this study demonstrated that pemetrexed is a rational option for salvage treatment, especially for second-line chemotherapy, when administered at a dose of 500 mg m−2 every 21 days to patients with metastatic gastric cancer. In this study, pemetrexed produced an ORR of 13.3% and DCR of 63.3% and the chemotherapy was well tolerated that there were no deaths resulted from toxicity.

During the progress of our study, results from several phase 3 randomized clinical trials were reported, supported that savage chemotherapy could add to patients' survival benefits (Thuss-Patience ; Kang ). A survival advantage was seen from the German Arbeitsgemeinschaft Internistische Onkologie (AIO) trial for irinotecan vs best supportive care, with an increased OS of 1.6 months. However, the trial was closed prematurely after accrual of only 40 patients over the first 4 years. The median survival time of the treatment group was only 4.0 months, and irinotecan produced no objective responses and SD in 53% (Thuss-Patience ). Another phase 3 trial of 193 patients compared BSC combined with either docetaxel or irinotecan with BSC alone, reporting an OS improvement of 1.3 months (Kang ). A recently published phase 3 randomized controlled trial, COUGAR-02 trial, suggested that docetaxel can be recommended as an appropriate second-line treatment for patients with oesophagogastric adenocarcinoma (Ford ). Furthermore, the median OS in the docetaxel group was 5.2 months vs 3.6 months in the active symptom control group. Biological agents have also had an important role in second-line treatment of gastric cancer. The result of REGARD study suggested that ramucirumab, a monoclonal antibody VEGFR-2 antagonist, could significantly increase OS from 3.8 months to 5.2 months for patients with previously treated advanced gastric cancer, compared with the placebo (Fuchs ). The RAINBOW study showed that the combination of ramucirumab with paclitaxel significantly increases OS compared with placebo plus paclitaxel (Wilke ). Overall survival was significantly longer in the ramucirumab plus paclitaxel group than in the placebo plus paclitaxel group (median 9.6 months vs 7.4 months).

Although second-line chemotherapy is active for advanced gastric cancer, the standard treatment strategy is still debating. WJOG4007 trial, a randomized phase 3 head-to-head trial, designed to compare two second-line regimens CPT-11 and wPTX for AGC, did not demonstrate the superiority one, indicating that the different choices of chemotherapy seem not to impact on the outcome. Moreover, the patients involved in the clinical trials were in good physical condition, whereas in clinical practice there were patients with poor status due to disease progression and previous treatment. For instance, for patients with ascites, oedema, intestinal obstruction or poor bone marrow function, taxanes or iritecan, which was associated with higher incidence of grade 3–4 neutropenia or diarrhoea, might not be a good choice because of the toxicity. Recently, multiple new drugs including S-1, oxaliplatin and pemetrexed have proved to be effective and tolerated in gastric cancer (Suh ; Barone ; Ishigami ; Chon ). Therefore, more treatment choices of effective salvage regimens with acceptable toxicity for previously treated metastatic gastric cancer are deserved exploring.

For pretreated patients with metastatic gastric cancer, monochemotherapy may be a reasonable choice as second-line treatment, as patients may have worse condition and lower tolerability of further chemotherapy. For such patients, response rate is not most important as in first-line treatment. Chemotherapy with less toxicity, better disease control and life prolongation should be considered preferentially. Some phase II trials showed that combination chemotherapy achieved no marked survival benefits in the second-line setting (Takiuchi, 2011; Kang ). Therefore, we chose not combination but single agent for the second-line treatment. For similar reasons, we set the dosage of 500 mg m−2 for pemetrexed, referenced from previous studies in which pemetrexed achieved a modest activity with acceptable toxicity (Hanauske ; Bajetta ).

As the patients enroled in our study were treated with one or more prior chemotherapy, the activity of pemetrexed may be modest compared with that in previous pemetrexed trials in first-line setting. But when compared with other second-line chemotherapy regimens, pemetrexed achieved a comparable activity. The common second-line regimens, commonly based on taxanes, iritecan, platinum and 5-FU, administered in combination or as single agent, produced a median OS of about 5–13 months, median PFS of about 2–4 months and ORR of about 5–30% (Wesolowski ; Baek ). Despite the difference in regimens, the activity might also be influenced by some potential prognostic factors, including time to progression on first-line treatment, serum albumin, performance status and chemotherapy-free interval. Considering that one-third patients enroled in the study were pretreated with two or more chemotherapy, our study showed that pemtrexed produced a considerable efficacy, with a median OS of 6.4 months, median PFS of 2.2 months and ORR of 13.3% in all patients and a median OS of 6.4 months, median PFS of 2.3 months and ORR of 18.2% in second-line group.

In summary, the preliminary activity and manageable toxicity observed in this study suggest that pemetrexed (500 mg m−2) has a therapeutic role in salvage treatment of metastatic gastric cancer. Further investigations are needed to compare pemetrexed with other agents in salvage treatment of gastric cancer.