Literature DB >> 2547324

An assay for superoxide dismutase activity in mammalian tissue homogenates.

D R Spitz1, L W Oberley.   

Abstract

During the course of measuring superoxide dismutase (SOD) activity in rat breast tissue, interferences in the nitroblue tetrazolium (NBT) and cytochrome c assay systems were noted. These interferences inhibit accurate measurement of SOD activity in breast tissues, necessitating the development of a new NBT-based assay that includes compounds capable of inhibiting tissue specific interferences. The most effective compounds were metal chelators that were also electron transport chain inhibitors. Bathocuproine sulfonate (BCS) was the most effective of these compounds. The inclusion of BCS in the NBT assay system was shown to make the accurate measurement of SOD activity in tissues with interferences possible.

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Year:  1989        PMID： 2547324     DOI： 10.1016/0003-2697(89)90192-9

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  164 in total

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Authors:  Robin J Mockett; Barbara H Sohal; Rajindar S Sohal
Journal:  Free Radic Biol Med       Date:  2010-10-19       Impact factor: 7.376

2.  Adaptive responses and apoptosis in endothelial cells exposed to carbon monoxide.

Authors:  S R Thom; D Fisher; Y A Xu; K Notarfrancesco; H Ischiropoulos
Journal:  Proc Natl Acad Sci U S A       Date:  2000-02-01       Impact factor: 11.205

3.  MK-801 effect on regional cerebral oxidative stress rate induced by different duration of global ischemia in gerbils.

Authors:  Vesna Selakovic; Branka Janac; Lidija Radenovic
Journal:  Mol Cell Biochem       Date:  2010-04-27       Impact factor: 3.396

4.  Sirt3-mediated deacetylation of evolutionarily conserved lysine 122 regulates MnSOD activity in response to stress.

Authors:  Randa Tao; Mitchell C Coleman; J Daniel Pennington; Ozkan Ozden; Seong-Hoon Park; Haiyan Jiang; Hyun-Seok Kim; Charles Robb Flynn; Salisha Hill; W Hayes McDonald; Alicia K Olivier; Douglas R Spitz; David Gius
Journal:  Mol Cell       Date:  2010-12-22       Impact factor: 17.970

5.  Manganese superoxide dismutase is a p53-regulated gene that switches cancers between early and advanced stages.

Authors:  Sanjit K Dhar; Jitbanjong Tangpong; Luksana Chaiswing; Terry D Oberley; Daret K St Clair
Journal:  Cancer Res       Date:  2011-10-18       Impact factor: 12.701

6.  Beta-amyloid mediated nitration of manganese superoxide dismutase: implication for oxidative stress in a APPNLH/NLH X PS-1P264L/P264L double knock-in mouse model of Alzheimer's disease.

Authors:  Muthuswamy Anantharaman; Jitbanjong Tangpong; Jeffery N Keller; Michael P Murphy; William R Markesbery; Kelley K Kiningham; Daret K St Clair
Journal:  Am J Pathol       Date:  2006-05       Impact factor: 4.307

7.  A role for mitochondrial dysfunction in perpetuating radiation-induced genomic instability.

Authors:  Grace J Kim; Gary M Fiskum; William F Morgan
Journal:  Cancer Res       Date:  2006-11-01       Impact factor: 12.701

8.  Immunogold analysis of antioxidant enzymes in human renal cell carcinoma.

Authors:  T D Oberley; J M Sempf; M J Oberley; M L McCormick; K E Muse; L W Oberley
Journal:  Virchows Arch       Date:  1994       Impact factor: 4.064

9.  Measurement of superoxide dismutase, catalase and glutathione peroxidase in cultured cells and tissue.

Authors:  Christine J Weydert; Joseph J Cullen
Journal:  Nat Protoc       Date:  2009-12-17       Impact factor: 13.491

10.  Oxidative stress is involved in heat-induced cell death in Saccharomyces cerevisiae.

Authors:  J F Davidson; B Whyte; P H Bissinger; R H Schiestl
Journal:  Proc Natl Acad Sci U S A       Date:  1996-05-14       Impact factor: 11.205
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