CONTEXT: Tragopogon graminifolius DC. (Compositae) (TG) has been proposed as an efficacious remedy for gastrointestinal ulcers in Iranian traditional medicine. OBJECTIVE: The present study evaluates the efficacy of TG on experimental colitis and the responsible mechanisms. MATERIALS AND METHODS: After induction of IBD by 2,4,6-trinitrobenzenesulfonic acid (TNBS), rats received standardized ethanol extract of TG aerial part at 20, 30, or 50 mg/kg/d orally. After 12 d, the rats were sacrificed and the colon was removed and assessed for macroscopic and microscopic changes. Also, tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), total antioxidant capacity, myeloperoxidase (MPO), and lipid peroxidation (LPO) were measured in the colon homogenate. RESULT: TG extract significantly reduced macroscopic and microscopic scores of colitis with ED50 values of 23 and 39 mg/kg, respectively. MPO was significantly reduced in all plant extract groups with an ED50 value of 41 mg/kg. The ED50 values of extract for inhibition of TNF-α and LPO were 44 and 93 mg/kg, respectively. IL-1β significantly decreased by 50 mg/kg of TG extract (ED50 = 57 mg/kg). Total antioxidant power markedly increased by 50 mg/kg group (ED50 = 43 mg/kg). DISCUSSION: TG exhibited efficacy on TNBS-induced colitis via anti-inflammatory, immunomodulatory, antioxidant, and mucosal healing properties. CONCLUSION: TG possesses promising healing function on colitis. Clinical trials are warranted to prove its efficacy and tolerability in IBD.
CONTEXT: Tragopogon graminifolius DC. (Compositae) (TG) has been proposed as an efficacious remedy for gastrointestinal ulcers in Iranian traditional medicine. OBJECTIVE: The present study evaluates the efficacy of TG on experimental colitis and the responsible mechanisms. MATERIALS AND METHODS: After induction of IBD by 2,4,6-trinitrobenzenesulfonic acid (TNBS), rats received standardized ethanol extract of TG aerial part at 20, 30, or 50 mg/kg/d orally. After 12 d, the rats were sacrificed and the colon was removed and assessed for macroscopic and microscopic changes. Also, tumornecrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), total antioxidant capacity, myeloperoxidase (MPO), and lipid peroxidation (LPO) were measured in the colon homogenate. RESULT: TG extract significantly reduced macroscopic and microscopic scores of colitis with ED50 values of 23 and 39 mg/kg, respectively. MPO was significantly reduced in all plant extract groups with an ED50 value of 41 mg/kg. The ED50 values of extract for inhibition of TNF-α and LPO were 44 and 93 mg/kg, respectively. IL-1β significantly decreased by 50 mg/kg of TG extract (ED50 = 57 mg/kg). Total antioxidant power markedly increased by 50 mg/kg group (ED50 = 43 mg/kg). DISCUSSION: TG exhibited efficacy on TNBS-induced colitis via anti-inflammatory, immunomodulatory, antioxidant, and mucosal healing properties. CONCLUSION:TG possesses promising healing function on colitis. Clinical trials are warranted to prove its efficacy and tolerability in IBD.
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Keywords:
Herbal medicine; inflammatory bowel disease; inflammatory cytokines; medicinal plant; neutrophil infiltration; oxidative stress; phenolic compounds; traditional medicine
Authors: Roodabeh Bahramsoltani; Mohammad Hosein Farzaei; Amir Hossein Abdolghaffari; Roja Rahimi; Nasrin Samadi; Mohammad Heidari; Mohammadamin Esfandyari; Maryam Baeeri; Gholamreza Hassanzadeh; Mohammad Abdollahi; Saba Soltani; Ali Pourvaziri; Gholamreza Amin Journal: Iran J Basic Med Sci Date: 2017-07 Impact factor: 2.699
Authors: Fatemeh Dolatabadi; Amir H Abdolghaffari; Mohammad H Farzaei; Maryam Baeeri; Fatemeh S Ziarani; Majid Eslami; Mohammad Abdollahi; Roja Rahimi Journal: J Neurogastroenterol Motil Date: 2018-07-30 Impact factor: 4.924