| Literature DB >> 2547119 |
S Murai1, H Miyate, H Saito, H Nagahama, Y Masuda, T Itoh.
Abstract
A rapid and simple method using a combination of high-performance liquid chromatography (HPLC) with electrochemical detection (ECD) and an immobilized enzyme column herein is described for simultaneous assay of acetylcholine (ACh) and choline (Ch) in discrete brain areas of the mouse. Perchloric acid extracts of small brain tissues were injected directly onto the HPLC system with no pre-cleanup procedure. The ACh and Ch levels were determined within 4 min for each chromatographic run. Recoveries were 100.0 +/- 4.8% for ACh and 96.1 +/- 1.4% (mean +/- S.D., n = 10) for Ch. Detection limits for both compounds were 0.25 pmol (signal-to-noise ratio of three). The present method has been applied to the measurement of Ch and ACh in several discrete brain areas of the mouse.Entities:
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Year: 1989 PMID： 2547119 DOI： 10.1016/0160-5402(89)90063-6
Source DB: PubMed Journal: J Pharmacol Methods ISSN： 0160-5402