Macrophage-produced oxygen radical generating activities for polymorphonuclear leukocytes.

Guinea pig peritoneal macrophages were stimulated in vitro by bacterial lipopolysaccharide. After incubation, the supernatants of macrophage cultures were collected and tested for O2- production on guinea pig peritoneal polymorphonuclear leukocytes. The supernatants of macrophage cultures stimulated by lipopolysaccharide had significantly higher levels of O2- -generating activities in polymorphonuclear leukocytes, and these activities appeared in the macrophage cultures within 2 h after stimulation by lipopolysaccharide. However, the supernatants obtained from the nonstimulated cultures could not produce these activities. These activities disappeared with heating or trypsin and were not produced in macrophage cultures by incubation with cycloheximide.