Literature DB >> 25466441

Capture and Release (CaR): a simplified procedure for one-tube isolation and concentration of single-stranded DNA during SELEX.

Nasim Shahidi Hamedani1, Fabian Blümke, Fabian Tolle, Falk Rohrbach, Heiko Rühl, Johannes Oldenburg, Günter Mayer, Bernd Pötzsch, Jens Müller.   

Abstract

Short biotinylated oligodeoxynucleotides immobilized on streptavidin-coated magnetic beads allow for convenient and rapid purification of single-stranded oligodeoxynucleotides from crude asymmetric PCR mixtures, facilitating the selection of DNA aptamers.

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Year:  2015        PMID： 25466441     DOI： 10.1039/c4cc08233h

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  4 in total

1.  Identification and characterization of nucleobase-modified aptamers by click-SELEX.

Authors:  Franziska Pfeiffer; Fabian Tolle; Malte Rosenthal; Gerhard Markus Brändle; Jörg Ewers; Günter Mayer
Journal:  Nat Protoc       Date:  2018-04-26       Impact factor: 13.491

Review 2.  Inside the Black Box: What Makes SELEX Better?

Authors:  Natalia Komarova; Alexander Kuznetsov
Journal:  Molecules       Date:  2019-10-07       Impact factor: 4.411

3.  Functional and Structural Characterization of Nucleic Acid Ligands That Bind to Activated Coagulation Factor XIII.

Authors:  Nasim Shahidi Hamedani; Arijit Biswas; Oliver Rudan; Rosa Tönges; Carlotta Meyring; Fabian Tolle; Günter Mayer; Johannes Oldenburg; Jens Müller; Bernd Pötzsch
Journal:  J Clin Med       Date:  2021-02-10       Impact factor: 4.241

4.  Aptamer loaded superparamagnetic beads for selective capturing and gentle release of activated protein C.

Authors:  Nasim Shahidi Hamedani; Felix Lucian Happich; Eva-Maria Klein; Heiko Rühl; Günter Mayer; Johannes Oldenburg; Jens Müller; Bernd Pötzsch
Journal:  Sci Rep       Date:  2022-04-30       Impact factor: 4.996
  4 in total

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