| Literature DB >> 25464850 |
Li-Ying Sung1, Wei-Fang Chang2, Qian Zhang3, Chia-Chia Liu4, Jun-Yang Liou5, Chia-Chun Chang2, Huan Ou-Yang2, Renpeng Guo3, Haifeng Fu3, Winston T K Cheng6, Shih-Torng Ding2, Chuan-Mu Chen7, Maja Okuka8, David L Keefe9, Y Eugene Chen10, Lin Liu11, Jie Xu12.
Abstract
Haplo-insufficiency of telomerase genes in humans leads to telomere syndromes such as dyskeratosis congenital and idiopathic pulmonary fibrosis. Generation of pluripotent stem cells from telomerase haplo-insufficient donor cells would provide unique opportunities toward the realization of patient-specific stem cell therapies. Recently, pluripotent human embryonic stem cells (ntESCs) have been efficiently achieved by somatic cell nuclear transfer (SCNT). We tested the hypothesis that SCNT could effectively elongate shortening telomeres of telomerase haplo-insufficient cells in the ntESCs with relevant mouse models. Indeed, telomeres of telomerase haplo-insufficient (Terc(+/-)) mouse cells are elongated in ntESCs. Moreover, ntESCs derived from Terc(+/-) cells exhibit naive pluripotency as evidenced by generation of Terc(+/-) ntESC clone pups by tetraploid embryo complementation, the most stringent test of naive pluripotency. These data suggest that SCNT could offer a powerful tool to reprogram telomeres and to discover the factors for robust restoration of telomeres and pluripotency of telomerase haplo-insufficient somatic cells.Entities:
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Year: 2014 PMID： 25464850 PMCID： PMC4268138 DOI： 10.1016/j.celrep.2014.10.052
Source DB: PubMed Journal: Cell Rep Impact factor: 9.423