| Literature DB >> 25462642 |
Henry Jay Forman1, Ohara Augusto2, Regina Brigelius-Flohe3, Phyllis A Dennery4, Balaraman Kalyanaraman5, Harry Ischiropoulos6, Giovanni E Mann7, Rafael Radi8, L Jackson Roberts9, Jose Vina10, Kelvin J A Davies11.
Abstract
Free radicals and oxidants are now implicated in physiological responses and in several diseases. Given the wide range of expertise of free radical researchers, application of the greater understanding of chemistry has not been uniformly applied to biological studies. We suggest that some widely used methodologies and terminologies hamper progress and need to be addressed. We make the case for abandonment and judicious use of several methods and terms and suggest practical and viable alternatives. These changes are suggested in four areas: use of fluorescent dyes to identify and quantify reactive species, methods for measurement of lipid peroxidation in complex biological systems, claims of antioxidants as radical scavengers, and use of the terms for reactive species.Entities:
Keywords: Antioxidant; F2-isoprostane; Fluorescent dye; Free radicals; Radical scavenging; Reactive nitrogen species; Reactive oxygen species; Thiobarbituric acid; α-Tocopherol
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Year: 2014 PMID： 25462642 DOI： 10.1016/j.freeradbiomed.2014.10.504
Source DB: PubMed Journal: Free Radic Biol Med ISSN： 0891-5849 Impact factor: 7.376