Prasanna Neelakantan1, Cheng Qing Cheng2, Vinoddhine Ravichandran2, Teresa Mao2, Priyanka Sriraman2, Swetha Sridharan2, Chandana Subbarao2, Subash Sharma2, Anil Kishen3. 1. Biofilm Research Cluster and Department of Conservative Dentistry and Endodontics, Saveetha Dental College and Hospitals, Saveetha University, Chennai, India. Electronic address: prasanna_neelakantan@yahoo.com. 2. Biofilm Research Cluster and Department of Conservative Dentistry and Endodontics, Saveetha Dental College and Hospitals, Saveetha University, Chennai, India. 3. Discipline of Endodontics, Faculty of Dentistry, University of Toronto, Toronto, Canada.
Abstract
BACKGROUND: To test the effect of ultrasonic or light activated curcumin and sodium hypochlorite against Enterococcus faecalis biofilms in vitro. METHODS: E. faecalis biofilms were grown within root canals (n=175) and divided into 7 groups (n=25). Group 1, sterile saline; group 2, 3% sodium hypochlorite; group 3, 3% sodium hypochlorite activated with ultrasonic files (30s cycles for 4min); group 4, 3% sodium hypochlorite irradiated with blue light (1200mw/cm(2) for 4min); group 5, curcumin (2.5mg/mL); group 6, curcumin (2.5mg/mL) activated with ultrasonic files (30s cycles for 4min); group 7, curcumin (2.5mg/mL) irradiated with blue light. The biofilms' ultrastructure was examined using scanning electron microscopy. Bacterial viability was assessed by confocal microscopy. Data were analyzed by one-way ANOVA and Student-Newman-Keuls test (P=0.05). The quantitative analysis of the colony-forming units was carried out from dentinal shaving and analyzed by One-way ANOVA and Tukey multiple comparison test (P=0.05). RESULTS: All treatment groups showed a significantly higher percentage of dead bacteria than the saline control (P<0.05). The percentage of dead bacteria was significantly higher when light activated curcumin was used (P<0.05). At both depths (200 and 400 microns), light activated curcumin showed no growth of bacteria. CONCLUSIONS: Light activation produced significantly higher antibacterial efficacy than ultrasonic agitation, with light activated curcumin producing the maximum elimination of biofilm bacteria within the root canal lumen and dentinal tubules.
BACKGROUND: To test the effect of ultrasonic or light activated curcumin and sodium hypochlorite against Enterococcus faecalis biofilms in vitro. METHODS:E. faecalis biofilms were grown within root canals (n=175) and divided into 7 groups (n=25). Group 1, sterile saline; group 2, 3% sodium hypochlorite; group 3, 3% sodium hypochlorite activated with ultrasonic files (30s cycles for 4min); group 4, 3% sodium hypochlorite irradiated with blue light (1200mw/cm(2) for 4min); group 5, curcumin (2.5mg/mL); group 6, curcumin (2.5mg/mL) activated with ultrasonic files (30s cycles for 4min); group 7, curcumin (2.5mg/mL) irradiated with blue light. The biofilms' ultrastructure was examined using scanning electron microscopy. Bacterial viability was assessed by confocal microscopy. Data were analyzed by one-way ANOVA and Student-Newman-Keuls test (P=0.05). The quantitative analysis of the colony-forming units was carried out from dentinal shaving and analyzed by One-way ANOVA and Tukey multiple comparison test (P=0.05). RESULTS: All treatment groups showed a significantly higher percentage of dead bacteria than the saline control (P<0.05). The percentage of dead bacteria was significantly higher when light activated curcumin was used (P<0.05). At both depths (200 and 400 microns), light activated curcumin showed no growth of bacteria. CONCLUSIONS: Light activation produced significantly higher antibacterial efficacy than ultrasonic agitation, with light activated curcumin producing the maximum elimination of biofilm bacteria within the root canal lumen and dentinal tubules.
Authors: Yucheng Wang; Ying Wang; Yuguang Wang; Clinton K Murray; Michael R Hamblin; David C Hooper; Tianhong Dai Journal: Drug Resist Updat Date: 2017-10-13 Impact factor: 18.500
Authors: Julian M Sotomil; Eliseu A Münchow; Divya Pankajakshan; Kenneth J Spolnik; Jessica A Ferreira; Richard L Gregory; Marco C Bottino Journal: J Endod Date: 2019-09-18 Impact factor: 4.171
Authors: Imran Ahmed; Yanyan Fang; Min Lu; Quan Yan; Ahmed El-Hussein; Michael R Hamblin; Tianhong Dai Journal: Recent Pat Antiinfect Drug Discov Date: 2018